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HSCB Antibody (OTI3E1)

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Western Blot: HSCB Antibody (3E1) [NBP2-46506] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSCB.
Immunohistochemistry-Paraffin: HSCB Antibody (3E1) [NBP2-46506] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Western Blot: HSCB Antibody (3E1) [NBP2-46506] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: ...read more
Immunohistochemistry-Paraffin: HSCB Antibody (3E1) [NBP2-46506] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry-Paraffin: HSCB Antibody (3E1) [NBP2-46506] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry-Paraffin: HSCB Antibody (3E1) [NBP2-46506] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry-Paraffin: HSCB Antibody (3E1) [NBP2-46506] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry-Paraffin: HSCB Antibody (3E1) [NBP2-46506] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, IHC
Clone
OTI3E1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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HSCB Antibody (OTI3E1) Summary

Immunogen
Full length human recombinant protein of human HSCB(NP_741999) produced in HEK293T cell
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HSCB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:400-4000
Theoretical MW
27.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HSCB Antibody (OTI3E1)

  • DnaJ homolog subfamily C member 20
  • DNAJC20DnaJ (Hsp40) homolog, subfamily C, member 20
  • Hsc20
  • HSC20dJ366L4.2
  • HscB iron-sulfur cluster co-chaperone homolog (E. coli)
  • iron-sulfur cluster co-chaperone protein HscB, mitochondrial
  • JAC1
  • J-type co-chaperone HSC20
  • MGC2637
  • MGC74462

Background

HSCB may act as a co-chaperone in iron-sulfur cluster assembly in mitochondria

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HSCB