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ELOVL1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: ELOVL1 Antibody [NBP2-37956] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Orthogonal Strategies: Immunohistochemistry-Paraffin: ELOVL1 Antibody [NBP2-37956] - Staining in human skin and skeletal muscle tissues . Corresponding ELOVL1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ELOVL1 Antibody [NBP2-37956] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ELOVL1 Antibody [NBP2-37956] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: ELOVL1 Antibody [NBP2-37956] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
Immunohistochemistry-Paraffin: ELOVL1 Antibody [NBP2-37956] - Staining of human skin shows strong granular cytoplasmic positivity in sebaceous gland and keratinocytes.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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ELOVL1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: FWYHSYTKGKRLPRALQQNGAPGIAKVK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ELOVL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ELOVL1 Protein (NBP2-37956PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (82%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ELOVL1 Antibody

  • EC 2.3.1.n8
  • elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 1
  • elongation of very long chain fatty acids protein 1
  • SSC1
  • Ssc1,3-keto acyl-CoA synthase ELOVL1

Background

Elongation of very long chain fatty acid-like (ELOVL) proteins 1-6 are members of the ELO family of proteins, which play an important role in tissue-specific biosynthesis of very long chain fatty acids and sphingolipids. The ELOVL proteins act as catalysts in fatty acid elongation reduction and localize to the endoplasmic reticulum (ER). Elongation of very long chain fatty acids protein 1 (ELOVL1), also referred to as Ssc1, is the human homolog of the yeast ELO3 protein. It is expressed in a variety of tissues and at especially high levels in stomach, skin, intestine, kidney and lung. ELOVL1 participates in the elongation of very long chain saturated and monounsaturated fatty acids of up to 26 carbons and may be required for the development of a barrier in epithelial cells and skin. ELOVL1 is also important for the formation of Myelin in the central nervous system. Impaired ELOVL1 activity may be associated with disorders of sphingolipid metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ELOVL1
Uniprot