![Western Blot: HSP90 alpha Antibody [NBP2-16895] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 alpha antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Western-Blot-NBP2-16895-img0017.jpg "Western Blot: HSP90 alpha Antibody [NBP2-16895] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 alpha antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Dr, In, Pm, Po, Bv, Ch, Pm, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HSP90 alpha. The exact sequence is proprietary. |
| Localization | Cytoplasm; Melanosome |
| Predicted Species | Porcine (100%), Chimpanzee (99%), Bovine (99%), Rhesus Macaque (100%), Chicken (90%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HSP90AA1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 85 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for HSP90 alpha Antibody (NBP2-16895)Find related products by research area.
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Hsp90A - helping to fold a wide variety of signal transduction proteins Heat-shock protein 90 (Hsp90) is a conserved ATP-dependent chaperone with diverse cellular functions. Hsp90 is ubiquitously expressed but is further induced upon cellular stress such as heat-shock or nutrient deprivation. Hsp90 is essential for the... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: HSP90 alpha Antibody [NBP2-16895] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hsp90 alpha stained by Hsp90 alpha antibody diluted at 1:500. lue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16895-img0018.jpg "Immunocytochemistry/Immunofluorescence: HSP90 alpha Antibody [NBP2-16895] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hsp90 alpha stained by Hsp90 alpha antibody diluted at 1:500. lue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0022.jpg "Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: HSP90 alpha Antibody [NBP2-16895] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 alpha antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Western-Blot-NBP2-16895-img0015.jpg "Western Blot: HSP90 alpha Antibody [NBP2-16895] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 alpha antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - Paraffin-embedded human colon carcinoma. Hsp90 alpha antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0012.jpg "Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - Paraffin-embedded human colon carcinoma. Hsp90 alpha antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0019.jpg "Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0020.jpg "Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0021.jpg "Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunoprecipitation: HSP90 alpha Antibody [NBP2-16895] - Immunoprecipitation of Hsp90 alpha protein from 293T whole cell extracts using 5 ug of Hsp90 alpha antibody. Western blot analysis was performed using Hsp90 alpha antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunoprecipitation-NBP2-16895-img0011.jpg "Immunoprecipitation: HSP90 alpha Antibody [NBP2-16895] - Immunoprecipitation of Hsp90 alpha protein from 293T whole cell extracts using 5 ug of Hsp90 alpha antibody. Western blot analysis was performed using Hsp90 alpha antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.")
![Western Blot: HSP90 alpha Antibody [NBP2-16895] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 alpha antibody (NBP2-16895) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16895_rabbit-polyclonal-hsp90-alpha-antibody-91020241957223.jpg "Western Blot: HSP90 alpha Antibody [NBP2-16895] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 alpha antibody (NBP2-16895) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")