HSP90 alpha Antibodies
We offer HSP90 alpha Antibodies for use in common research applications: Chromatin Immunoprecipitation, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot. Each HSP90 alpha Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our HSP90 alpha Antibodies can be used in a variety of model species: Chicken, Human, Mouse, Primate, Rabbit, Rat, Sheep. Use the list below to choose the HSP90 alpha Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our HSP90 alpha polyclonal antibodies and browse our HSP90 alpha monoclonal antibody catalog.
Alternate Names for HSP90 alpha Antibodies
anti-HSP90 alpha antibody, anti-HSP90AA1 antibody, anti-FLJ31884 antibody, anti-Heat shock 86 kDa antibody, anti-heat shock 90kD protein 1, alpha antibody, anti-heat shock 90kD protein 1, alpha-like 4 antibody, anti-heat shock 90kD protein, alpha-like 4 antibody, anti-heat shock 90kDa protein 1, alpha antibody, anti-heat shock protein 90kDa alpha (cytosolic), class A member 1 antibody, anti-heat shock protein HSP 90-alpha antibody, anti-Hsp89 antibody, anti-Hsp90 antibody, anti-HSP90AHSP86 antibody, anti-HSP90N antibody, anti-HSPC1HSP 86 antibody, anti-HSPCAHSP89A antibody, anti-HSPCAL1 antibody, anti-HSPCAL4 antibody, anti-HSPN antibody, anti-LAP2 antibody, anti-Renal carcinoma antigen NY-REN-38 antibody
15 Results for "hsp90-alpha" in Primary Antibodies
![Western Blot: HSP90 alpha Antibody [NB120-2928] - Analysis of 2-fold serial dilutions of HeLa cell lysate, starting at 10 ug, per well.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Western-Blot-NB120-2928-img0023.jpg "Western Blot: HSP90 alpha Antibody [NB120-2928] - Analysis of 2-fold serial dilutions of HeLa cell lysate, starting at 10 ug, per well.")
![Immunocytochemistry/Immunofluorescence: HSP90 alpha Antibody [NB120-2928] - Analysis of Heat Shock Protein 86 (HSP86, green) in HeLa cells and NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a HSP86 polyclonal antibody, at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB120-2928-img0012.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Signaling consequences of STA-8666 treatment. HSP90 alpha. Magnification: 20x. Scale bars: 75um, except for vimentin, where scale bar is 40um. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0176747) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NB120-2928-img0026.jpg)
![Western Blot: HSP90 alpha Antibody [NB120-2928] - Figure 1 shows a Western blot of mouse HSP84 and HSP86 using NB120-2928 and NB120-2927 respectively.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Western-Blot-NB120-2928-img0015.jpg)
![Western Blot: HSP90 alpha Antibody [NB120-2928] - Analysis of epidermoid carcinoma (A431) cells using Heat Shock Protein 86 Polyclonal Antibody. Decreasing amounts of A431 whole cell lysates were probed with PA3-013 at a dilution of 1:2000 followed by an anti-rabbit IgG secondary antibody and SuperSignal West Pico Chemiluminescent Substrate. Lanes 1 and 2 are control samples of purified, recombinant Hsp86 (rHsp86) and Hsp84 (rHsp84), respectively. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Western-Blot-NB120-2928-img0016.jpg)
![Western Blot: HSP90 alpha Antibody [NB120-2928] - Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP86 polyclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-rabbit IgG HRP secondary antibody at a dilution of 1:20,000 for at least 1 hour.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Western-Blot-NB120-2928-img0017.jpg)
![Western Blot: HSP90 alpha Antibody [NB120-2928] - Analysis of 2-fold serial dilutions of HeLa cell lysate, starting at 10 ug, per well.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Western-Blot-NB120-2928-img0022.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Both normal and cancer biopsies of deparaffinized Human kidney tissues.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NB120-2928-img0010.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NB120-2928-img0018.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Heat-induced epitope retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 20 mins at 95C. Following antigen retrieval, tissues blocked in 3% BSA in PBST for 30 mins at RT & then probed with Heat Shock Protein 86 (Hsp86) polyclonal antibody at 1:100 for 1 hr in a humidified chamber (right panel). As a -ve control, primary antibody eliminated from the staining procedure (left panel). Tissues washed with PBS/0.025% Tween-20 & endogenous peroxidase activity quenched with Peroxidase Suppressor for 30 mins at RT. Detection performed using an HRP-conjugated goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:250 followed by colorimetric detection using Metal Enhanced DAB Substrate Kit. Tissues were counterstained with hematoxylin & prepped for mouting. Images were taken on a Zeiss Axiovision microscope at 40X magnification (x1.6 Optovar).](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NB120-2928-img0019.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Both normal and cancer biopsies of deparaffinized Human breast carcinoma tissues.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NB120-2928-img0020.jpg)
![Immunohistochemistry: HSP90 alpha Antibody [NB120-2928] - HSP90 alpha is upregulated in epithelial cells lining liver cysts.(A, B) Representative hematoxylin stained liver sections with immunohistochemical detection of HSP90 alpha (brown) from three (A) wild type (wt) and (B) Pkd1-/- mice. bd = bile duct, pv = portal vein, c = cyst. Scale bars = 100 um; magnification, 20x. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0114403) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-NB120-2928-img0024.jpg)
![Immunohistochemistry: HSP90 alpha Antibody [NB120-2928] - HSP90 alpha is upregulated in epithelial cells lining liver cysts.(A, B) Representative hematoxylin stained liver sections with immunohistochemical detection of HSP90 alpha (brown) from three (A) wild type (wt) and (B) Pkd1-/- mice. bd = bile duct, pv = portal vein, c = cyst. Scale bars = 100 um; magnification, 20x. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0114403) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-NB120-2928-img0025.jpg)
![Immunoprecipitation: HSP90 alpha Antibody [NB120-2928] - Analysis of Heat Shock Protein (HSP86) was performed on HeLa cells. Antigen-antibody complexes formed by incubating 500ug whole cell lysate with 2ug of HSP86 polyclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with Lane Marker Reducing Sample Buffer. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP86 polyclonal antibody at a dilution of 1:1000 overnight rotating at 4C. The membrane was washed in TBST, and probed with Clean-Blot IP detection Reagent at a dilution of 1:1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunoprecipitation-NB120-2928-img0021.jpg)
![Western Blot: HSP90 alpha Antibody [NBP2-16895] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 alpha antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Western-Blot-NBP2-16895-img0017.jpg "Western Blot: HSP90 alpha Antibody [NBP2-16895] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 alpha antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: HSP90 alpha Antibody [NBP2-16895] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hsp90 alpha stained by Hsp90 alpha antibody diluted at 1:500. lue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16895-img0018.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0022.jpg)
![Western Blot: HSP90 alpha Antibody [NBP2-16895] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 alpha antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Western-Blot-NBP2-16895-img0015.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - Paraffin-embedded human colon carcinoma. Hsp90 alpha antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0012.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0019.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0020.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP2-16895] - HSP90 alpha antibody detects HSP90 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. HSP90 alpha stained by HSP90 alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16895-img0021.jpg)
![Immunoprecipitation: HSP90 alpha Antibody [NBP2-16895] - Immunoprecipitation of Hsp90 alpha protein from 293T whole cell extracts using 5 ug of Hsp90 alpha antibody. Western blot analysis was performed using Hsp90 alpha antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunoprecipitation-NBP2-16895-img0011.jpg)
![Western Blot: HSP90 alpha Antibody [NBP2-16895] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 alpha antibody (NBP2-16895) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16895_rabbit-polyclonal-hsp90-alpha-antibody-91020241957223.jpg)
![Immunocytochemistry/Immunofluorescence: HSP90 alpha Antibody [NBP1-77682] - HSP90-1A antibody was tested in MCF-7 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77682-img0005.jpg "Immunocytochemistry/Immunofluorescence: HSP90 alpha Antibody [NBP1-77682] - HSP90-1A antibody was tested in MCF-7 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).")
![Immunohistochemistry: HSP90 alpha Antibody [NBP1-77682] - Analysis of Hsp901a in human kidney carcinoma using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-NBP1-77682-img0006.jpg)
![Western Blot: HSP90 alpha Antibody (5G5) [NBP2-22208] - Western blot analysis using Hsp90A mouse mAb against NIH3T3 (1), HeLa (2), HCT116(3), HL-60 (4) and C0S7 (5) cell lysate.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-5G5-Western-Blot-NBP2-22208-img0006.jpg "Western Blot: HSP90 alpha Antibody (5G5) [NBP2-22208] - Western blot analysis using Hsp90A mouse mAb against NIH3T3 (1), HeLa (2), HCT116(3), HL-60 (4) and C0S7 (5) cell lysate.")
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody (5G5) [NBP2-22208] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using Hsp90A mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-5G5-Immunohistochemistry-Paraffin-NBP2-22208-img0003.jpg)
![Flow Cytometry: HSP90 alpha Antibody (5G5) [NBP2-22208] - Flow cytometric analysis of HeLa cells using Hsp90A mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-5G5-Flow-Cytometry-NBP2-22208-img0001.jpg)
![Western Blot: HSP90 alpha Antibody (5G5) [NBP2-22208] - Western blot analysis using Hsp90A mAb against human HSP90AA1 (AA: 275-484) recombinant protein. (Expected MW is 50.5 kDa)](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-5G5-Western-Blot-NBP2-22208-img0005.jpg)
![Immunohistochemistry-Paraffin: HSP90 alpha Antibody (5G5) [NBP2-22208] - Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using Hsp90A mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-5G5-Immunohistochemistry-Paraffin-NBP2-22208-img0002.jpg)
![ELISA: HSP90 alpha Antibody (5G5) [NBP2-22208] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-5G5-ELISA-NBP2-22208-img0004.jpg)
![Western Blot: HSP90 alpha Antibody (2G5.G3) [NBP2-12877] - Western Blot analysis of Rat tissue lysate showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone 2G5.G3 (NBP2-12877). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody (NBP2-12877) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-2G5-G3-Western-Blot-NBP2-12877-img0009.jpg "Western Blot: HSP90 alpha Antibody (2G5.G3) [NBP2-12877] - Western Blot analysis of Rat tissue lysate showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone 2G5.G3 (NBP2-12877). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody (NBP2-12877) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: HSP90 alpha Antibody (2G5.G3) [NBP2-12877] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-2G5-G3-Immunocytochemistry-Immunofluorescence-NBP2-12877-img0007.jpg)
![Western Blot: HSP90 alpha [ac Lys294] Antibody [NBP1-77944] - Shows detection of a band at ~90 kDa corresponding to Hsp90 in an SkBr3 cell lysate (arrowhead) after treatment with Trichostatin A (an HDAC inhibitor). Western blotting results do not definitively demonstrate the acetyl K294 specificity of this reagent because similar staining is seen in the control lane (no treatment with TSA). Immunoprecipitation with anti-Hsp90 was performed prior to western blotting with anti-Hsp90 acetyl K294.](http://images.novusbio.com/fullsize/HSP90-alpha-[ac-Lys294]-Antibody-Western-Blot-NBP1-77944-img0005.jpg "Western Blot: HSP90 alpha [ac Lys294] Antibody [NBP1-77944] - Shows detection of a band at ~90 kDa corresponding to Hsp90 in an SkBr3 cell lysate (arrowhead) after treatment with Trichostatin A (an HDAC inhibitor). Western blotting results do not definitively demonstrate the acetyl K294 specificity of this reagent because similar staining is seen in the control lane (no treatment with TSA). Immunoprecipitation with anti-Hsp90 was performed prior to western blotting with anti-Hsp90 acetyl K294.")
![Immunohistochemistry: HSP90 alpha [ac Lys294] Antibody [NBP1-77944] - Used at 20 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate nuclear and granular cytoplasmic positive staining in human prostate carcinoma at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/HSP90-alpha-[ac-Lys294]-Antibody-Immunohistochemistry-NBP1-77944-img0006.jpg)
![Western Blot: HSP90 alpha Antibody (Hyb-K41009) [NB110-61641] - analysis of Rat Lysates showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41009 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Hyb-K41009-Western-Blot-NB110-61641-img0007.jpg "Western Blot: HSP90 alpha Antibody (Hyb-K41009) [NB110-61641] - analysis of Rat Lysates showing detection of Hsp90 alpha protein using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41009 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry: HSP90 alpha Antibody (Hyb-K41009) [NB110-61641] - Immunohistochemistry analysis using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41009 (NB110-61641). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody (NB110-61641) at 1:5000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Hyb-K41009-Immunohistochemistry-NB110-61641-img0009.jpg)
![Immunohistochemistry: HSP90 alpha Antibody (Hyb-K41009) [NB110-61641] - Immunohistochemistry analysis using Mouse Anti-Hsp90 alpha Monoclonal Antibody, Clone K41009 (NB110-61641). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 alpha Monoclonal Antibody (NB110-61641) at 1:5000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Hyb-K41009-Immunohistochemistry-NB110-61641-img0010.jpg)
![Western Blot: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Western blot analysis of extracts of various cell lines, using HSP90 alpha Rabbit mAb (NBP3-16528) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-0R3S6-Western-Blot-NBP3-16528-img0001.jpg "Western Blot: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Western blot analysis of extracts of various cell lines, using HSP90 alpha Rabbit mAb (NBP3-16528) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunoprecipitation: HSP90 alpha Antibody (0R3S6) [NBP3-16528] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug HSP90 alpha antibody (NBP3-16528). Western blot was performed from the immunoprecipitate using HSP90 alpha antibody (NBP3-16528) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-0R3S6-Immunoprecipitation-NBP3-16528-img0002.jpg)
![Western Blot: HSP90 alpha Antibody (S06-9C1) [NBP3-19514] - Western blot detection of HSP90 alpha in K562, C6, Hela cell lysates using NBP3-19514 (1:1000 diluted). Predicted band size: 85kDa. Observed band size: 90kDa.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-S06-9C1-Western-Blot-NBP3-19514-img0001.jpg "Western Blot: HSP90 alpha Antibody (S06-9C1) [NBP3-19514] - Western blot detection of HSP90 alpha in K562, C6, Hela cell lysates using NBP3-19514 (1:1000 diluted). Predicted band size: 85kDa. Observed band size: 90kDa.")
