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HSF1 [p Ser326] Antibody

Images

 
Western Blot: HSF1 [p Ser326] Antibody [NBP1-97452] - Analysis of Recombinant HSF1 and HSF1 (phospho): Lane 1: MWM, Lane 2: Recombinant HSF1, Lane 3: Recombinant HSF1 (phospho), probed with HSF1 (pSer326) pAb.
Western Blot: HSF1 [p Ser326] Antibody [NBP1-97452] - Analysis of HSF1 (pSer326): Lane 1: MW marker, Lane 2: HeLa, Lane 3: HeLa, Heat-Shocked (No recovery time), probed with HSF1 (pSer326) pAb.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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HSF1 [p Ser326] Antibody Summary

Immunogen
Synthetic peptide corresponding to the N-terminus of human HSF1 phosphorylated at Ser 326.
Modification
p Ser326
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSF1
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Application Notes
Predicted MW of ~60kDa. Detects a band of 85-95kDa by Western blot depending on hyperphosphorylation of the native protein.
Publications
Read Publication using NBP1-97452.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 50% Glycerol
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Alternate Names for HSF1 [p Ser326] Antibody

  • heat shock factor protein 1
  • heat shock transcription factor 1HSTF1HSF 1
  • HSF1
  • HSTF 1
  • HSTF1

Background

HSFs (Heat Shock family of transcription factors), which consists of HSF 1-4, bind to highly conserved Heat shock elements (HSEs) in the promoter regions of heat shock genes, ultimately regulating the expression of Heat shock proteins (Hsps). On exposure to heat shock and other stresses, HSF1 localizes within seconds to discrete nuclear granules and on recovery from stress, HSF1 rapidly dissipates from the stress granules to a diffuse nucleoplasmic distribution.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HSF1 Antibody (NBP1-97452)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HSF1