HSF1 Antibodies
We offer HSF1 Antibodies for use in common research applications: ELISA, Enzyme Immunoassay, Flow Cytometry, Gel Super Shift Assays, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each HSF1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our HSF1 Antibodies can be used in a variety of model species: Gerbil, Human, Mouse, Primate, Rabbit, Rat. Use the list below to choose the HSF1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our HSF1 polyclonal antibodies and browse our HSF1 monoclonal antibody catalog.
Alternate Names for HSF1 Antibodies
anti-HSF1 antibody, anti-heat shock factor protein 1 antibody, anti-heat shock transcription factor 1HSTF1HSF 1 antibody, anti-HSTF 1 antibody
19 Results for "hsf1" in Primary Antibodies
![Western Blot: HSF1 Antibody [NBP1-97475] - Analysis of HSF1: Lane 1: Molecular Weight Marker, Lane 2: HSF1 (human), (recombinant), Lane 3: HeLa Cell Lysate, Lane 4: HeLa Cell Lysate (Heat Shocked), Lane 5: 3T3 Cell Lysate, Lane 6: PC-12 Cell Lysate, Lane 7: RK-13 Cell Lysate, Lane 8: Vero Cell Lysate.](http://images.novusbio.com/fullsize/HSF1-Antibody-Western-Blot-NBP1-97475-img0002.jpg "Western Blot: HSF1 Antibody [NBP1-97475] - Analysis of HSF1: Lane 1: Molecular Weight Marker, Lane 2: HSF1 (human), (recombinant), Lane 3: HeLa Cell Lysate, Lane 4: HeLa Cell Lysate (Heat Shocked), Lane 5: 3T3 Cell Lysate, Lane 6: PC-12 Cell Lysate, Lane 7: RK-13 Cell Lysate, Lane 8: Vero Cell Lysate.")
![Western Blot: HSF1 Antibody (4B4) [NBP2-42206] - Western Blot analysis of Human Breast adenocarcinoma cell line (MCF7) showing detection of ~65 kDa HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 4B4 (NBP2-42206). Lane 1: MW ladder. Lane 2: HSF1 null lysate prepared from mouse embryonic fibroblasts. Lane 3: MCF7 lysate (5 ug). Lane 4: MCF7 lysate (10 ug). Lane 5: MCF7 lysate (20 ug). Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (NBP2-42206) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rat IgG: HRP for 1 hour at RT. Predicted/Observed Size: ~65 kDa. Courtesy of: Dr. Sandro Santagata, Harvard Medical School.](http://images.novusbio.com/fullsize/HSF1-Antibody-4B4-Western-Blot-NBP2-42206-img0004.jpg "Western Blot: HSF1 Antibody (4B4) [NBP2-42206] - Western Blot analysis of Human Breast adenocarcinoma cell line (MCF7) showing detection of ~65 kDa HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 4B4 (NBP2-42206). Lane 1: MW ladder. Lane 2: HSF1 null lysate prepared from mouse embryonic fibroblasts. Lane 3: MCF7 lysate (5 ug). Lane 4: MCF7 lysate (10 ug). Lane 5: MCF7 lysate (20 ug). Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (NBP2-42206) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rat IgG: HRP for 1 hour at RT. Predicted/Observed Size: ~65 kDa. Courtesy of: Dr. Sandro Santagata, Harvard Medical School.")
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody (4B4) [NBP2-42206] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 100x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/HSF1-Antibody-4B4-Immunocytochemistry-Immunofluorescence-NBP2-42206-img0002.jpg)
![Western Blot: HSF1 Antibody (4B4) [NBP2-42206] - analysis of Human A431 and HEK293 cell lysates showing detection of HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 4B4 . Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/HSF1-Antibody-4B4-Western-Blot-NBP2-42206-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody (4B4) [NBP2-42206] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 20x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/HSF1-Antibody-4B4-Immunocytochemistry-Immunofluorescence-NBP2-42206-img0003.jpg)
![Western Blot: HSF1 Antibody (10H4) [NBP2-42205] - Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H4 (NBP2-42205). Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (NBP2-42205) at 1:1000.](http://images.novusbio.com/fullsize/HSF1-Antibody-10H4-Western-Blot-NBP2-42205-img0004.jpg "Western Blot: HSF1 Antibody (10H4) [NBP2-42205] - Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H4 (NBP2-42205). Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (NBP2-42205) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody (10H4) [NBP2-42205] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 100x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/HSF1-Antibody-10H4-Immunocytochemistry-Immunofluorescence-NBP2-42205-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody (10H4) [NBP2-42205] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 20x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/HSF1-Antibody-10H4-Immunocytochemistry-Immunofluorescence-NBP2-42205-img0003.jpg)
![Western Blot: HSF1 Antibody (JE44-79) [NBP3-32444] - Western blot analysis of HSF1 on different lysates with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: U-2 OS cell lysate Lane 3: MCF7 cell lysate Lane 4: HEK-293 cell lysate Lane 5: K-562 cell lysate Lane 6: Jurkat cell lysate Lane 7: A549 cell lysate Lane 8: HepG2 cell lysate Lane 9: LoVo cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 57 kDa Observed band size: 75 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32444) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32444_rabbit-hsf1-mab-je44-79-278202415242677.jpg "Western Blot: HSF1 Antibody (JE44-79) [NBP3-32444] - Western blot analysis of HSF1 on different lysates with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: U-2 OS cell lysate Lane 3: MCF7 cell lysate Lane 4: HEK-293 cell lysate Lane 5: K-562 cell lysate Lane 6: Jurkat cell lysate Lane 7: A549 cell lysate Lane 8: HepG2 cell lysate Lane 9: LoVo cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 57 kDa Observed band size: 75 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32444) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HSF1 Antibody (JE44-79) [NBP3-32444] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32444) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32444_rabbit-hsf1-mab-je44-79-29820241420917.jpg)
![Immunocytochemistry/ Immunofluorescence: HSF1 Antibody (JE44-79) [NBP3-32444] - Immunocytochemistry analysis of HeLa cells labeling HSF1 with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32444_rabbit-hsf1-mab-je44-79-2920241033401.jpg)
![Flow Cytometry: HSF1 Antibody (JE44-79) [NBP3-32444] - Flow cytometric analysis of HeLa cells labeling HSF1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32444, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32444_rabbit-hsf1-mab-je44-79-392024884655.jpg)
![Immunohistochemistry-Paraffin: HSF1 Antibody [NBP1-71931] - Detection of human HSF1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: an affinity purified rabbit anti-HSF1 used at a dilution of 1:5,000 (0.2ug/ml) (left), a different anti-HSF1 antibody used at a dilution of 1:5,000 (0.2ug/ml) (center) and Cat. NBP1-71931 used at a dilution of 1:1,000 (0.2ug/ml) (right). Detection: DAB](http://images.novusbio.com/fullsize/HSF1-Antibody-Immunohistochemistry-Paraffin-NBP1-71931-img0004.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: HSF1 Antibody [NBP1-71931] - Detection of human HSF1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: an affinity purified rabbit anti-HSF1 used at a dilution of 1:5,000 (0.2ug/ml) (left), a different anti-HSF1 antibody used at a dilution of 1:5,000 (0.2ug/ml) (center) and Cat. NBP1-71931 used at a dilution of 1:1,000 (0.2ug/ml) (right). Detection: DAB")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: HSF1 Antibody [NBP1-71931] - Samples: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- HSF1 Cat. No. Lot1 used at a dilution of 1:1,000 (1ug/ml) (left), Cat. No. Lot1 used at a dilution of 1:1,000 (1ug/ml) (center) and Cat. No. Lot1 used at a dilution](http://images.novusbio.com/fullsize/HSF1-Antibody-Immunohistochemistry-NBP1-71931-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: HSF1 Antibody [NBP1-71931] - Detection of human HSF1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-HSF1 antibody NBP1-71931 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. HSF1 was also immunoprecipitated by two other rabbit anti-HSF1 antibodies, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/HSF1-Antibody-Immunoprecipitation-NBP1-71931-img0003.jpg)
![Western Blot: HSF1 Antibody [NBP1-81665] - Analysis in human cell line PC-3.](http://images.novusbio.com/fullsize/HSF1-Antibody-Western-Blot-NBP1-81665-img0007.jpg "Western Blot: HSF1 Antibody [NBP1-81665] - Analysis in human cell line PC-3.")
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody [NBP1-81665] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/HSF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81665-img0008.jpg)
![Immunohistochemistry-Paraffin: HSF1 Antibody [NBP1-81665] - Staining of human kidney shows strong nuclear and moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/HSF1-Antibody-Immunohistochemistry-Paraffin-NBP1-81665-img0005.jpg)
![Immunohistochemistry-Paraffin: HSF1 Antibody (10H8) [NBP1-97504] - Analysis of human skeletal muscle tissue stained with HSF1, mAb (10H8) at 10ug/ml.](http://images.novusbio.com/fullsize/HSF1-Antibody-10H8-Immunohistochemistry-Paraffin-NBP1-97504-img0002.jpg "Immunohistochemistry-Paraffin: HSF1 Antibody (10H8) [NBP1-97504] - Analysis of human skeletal muscle tissue stained with HSF1, mAb (10H8) at 10ug/ml.")
![Western Blot: HSF1 [p Ser 326] Antibody (SR2246) [NBP3-21520] - Western blot analysis using NBP3-21520 in HeLa cell lysates treated with heat.](http://images.novusbio.com/fullsize/nbp3-21520_rabbit-hsf1-p-ser-326-mab-sr2246-57202316112194.jpg "Western Blot: HSF1 [p Ser 326] Antibody (SR2246) [NBP3-21520] - Western blot analysis using NBP3-21520 in HeLa cell lysates treated with heat.")
![Western Blot: HSF1 [p Ser326] Antibody [NBP1-97452] - Analysis of Recombinant HSF1 and HSF1 (phospho): Lane 1: MWM, Lane 2: Recombinant HSF1, Lane 3: Recombinant HSF1 (phospho), probed with HSF1 (pSer326) pAb.](http://images.novusbio.com/fullsize/HSF1-[p-Ser326]-Antibody-Western-Blot-NBP1-97452-img0002.jpg "Western Blot: HSF1 [p Ser326] Antibody [NBP1-97452] - Analysis of Recombinant HSF1 and HSF1 (phospho): Lane 1: MWM, Lane 2: Recombinant HSF1, Lane 3: Recombinant HSF1 (phospho), probed with HSF1 (pSer326) pAb.")
![Western Blot: HSF1 [p Ser326] Antibody [NBP1-97452] - Analysis of HSF1 (pSer326): Lane 1: MW marker, Lane 2: HeLa, Lane 3: HeLa, Heat-Shocked (No recovery time), probed with HSF1 (pSer326) pAb.](http://images.novusbio.com/fullsize/HSF1-[p-Ser326]-Antibody-Western-Blot-NBP1-97452-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: HSF1 Antibody (009) [NBP2-90081] - Staining of Human HSF1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HSF1 monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor® 549-conjugated Goat Anti-rabbit IgG secondary antibody (red).](http://images.novusbio.com/fullsize/HSF1-Antibody-009-Immunocytochemistry-Immunofluorescence-NBP2-90081-img0001.jpg "Immunocytochemistry/Immunofluorescence: HSF1 Antibody (009) [NBP2-90081] - Staining of Human HSF1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HSF1 monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor® 549-conjugated Goat Anti-rabbit IgG secondary antibody (red).")
![Immunohistochemistry-Paraffin: HSF1 Antibody (009) [NBP2-90081] - Staining of human HSF1 in human tonsil with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/HSF1-Antibody-009-Immunohistochemistry-Paraffin-NBP2-90081-img0003.jpg)
![Immunohistochemistry-Paraffin: HSF1 Antibody (009) [NBP2-90081] - Staining of human HSF1 in human placenta with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/HSF1-Antibody-009-Immunohistochemistry-Paraffin-NBP2-90081-img0002.jpg)