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HSF1 Antibody (10H8)

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Immunohistochemistry-Paraffin: HSF1 Antibody (10H8) [NBP1-97504] - Analysis of human skeletal muscle tissue stained with HSF1, mAb (10H8) at 10ug/ml.
Immunohistochemistry-Paraffin: HSF1 Antibody (10H8) [NBP1-97504] - Analysis of human skeletal muscle tissue stained with HSF1, mAb (10H8) at 10ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, RbSpecies Glossary
Applications WB, IHC
Clone
10H8
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

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HSF1 Antibody (10H8) Summary

Immunogen
Recombinant mouse HSF1.
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
HSF1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:1000
Application Notes
This antibody is useful in Immunohistochemistry (paraffin sections). Predicted MW of ~60kDa. Detects a band of ~85kDa by Western blot.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 50% Glycerol
Preservative
0.09% Sodium Azide
Purity
Protein G purified

Alternate Names for HSF1 Antibody (10H8)

  • heat shock factor protein 1
  • heat shock transcription factor 1HSTF1HSF 1
  • HSF1
  • HSTF 1
  • HSTF1

Background

HSFs (Heat Shock family of transcription factors), which consists of HSF 1-4, bind to highly conserved Heat shock elements (HSEs) in the promoter regions of heat shock genes, ultimately regulating the expression of Heat shock proteins (Hsps). On exposure to heat shock and other stresses, HSF1 localizes within seconds to discrete nuclear granules and on recovery from stress, HSF1 rapidly dissipates from the stress granules to a diffuse nucleoplasmic distribution.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HSF1