HSF1 Antibody (4B4) [DyLight 680] Summary
Immunogen |
Purified recombinant mouse HSF1 protein, epitope mapping to amino acids 425-439 |
Localization |
Cytoplasm , Nucleus |
Specificity |
Detects approx 85kDa (unstressed cell lysates) and approx 95kDa (heat shocked cell lysates). |
Isotype |
IgG1 |
Clonality |
Monoclonal |
Host |
Rat |
Gene |
HSF1 |
Purity |
Protein G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- ELISA
- Gel Super Shift Assays
- Immunohistochemistry
- Immunoprecipitation
|
Application Notes |
Optimal dilution of this antibody should be experimentally determined. |
Reactivity Notes
Immunogen displays sequence identity for non-tested species: Human, Rat, Bovine, Guinea Pig, Hamster, Monkey and Rabbit.
Packaging, Storage & Formulations
Storage |
Store at 4C in the dark. |
Buffer |
50mM Sodium Borate |
Preservative |
0.05% Sodium Azide |
Purity |
Protein G purified |
Alternate Names for HSF1 Antibody (4B4) [DyLight 680]
Background
Human cells respond to heat stress by inducing the binding of a pre-existing transcriptional activator (heat shock factor, HSF) to DNA (1). Induction of heat shock protein (HSP) gene expression by stress is initiated by binding of HSF1 to HSP gene promoters to increase their transcription. The cytoprotective functions of these HSPs are essential for cell survival, and thus it is critical that inducible HSP gene expression be executed rapidly and efficiently. There is an interaction between heat shock factor 1 (HSF1) and symplekin, a protein known to form a complex with the polyadenylation factors CstF and CPSF. HSF1-symplekin complexes are detected only after stress treatment, and these two proteins co-localize in punctate nuclear structures in stressed cells (2). A chaperone/Hsp functioning as repressor of heat shock transcription factor (HSF) could make activation of hsp genes dependent on protein unfolding. It has been concluded that Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of HSF1 (3).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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