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HSF1 Antibody (4B4) [DyLight 680]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Gp, Ha, Pm, RbSpecies Glossary
Applications ELISA, GS, IHC, IP
Clone
4B4
Clonality
Monoclonal
Host
Rat
Conjugate
DyLight 680

HSF1 Antibody (4B4) [DyLight 680] Summary

Immunogen
Purified recombinant mouse HSF1 protein, epitope mapping to amino acids 425-439
Localization
Cytoplasm , Nucleus
Specificity
Detects approx 85kDa (unstressed cell lysates) and approx 95kDa (heat shocked cell lysates).
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
HSF1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Gel Super Shift Assays
  • Immunohistochemistry
  • Immunoprecipitation
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Immunogen displays sequence identity for non-tested species: Human, Rat, Bovine, Guinea Pig, Hamster, Monkey and Rabbit.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for HSF1 Antibody (4B4) [DyLight 680]

  • heat shock factor protein 1
  • heat shock transcription factor 1HSTF1HSF 1
  • HSF1
  • HSTF 1
  • HSTF1

Background

Human cells respond to heat stress by inducing the binding of a pre-existing transcriptional activator (heat shock factor, HSF) to DNA (1). Induction of heat shock protein (HSP) gene expression by stress is initiated by binding of HSF1 to HSP gene promoters to increase their transcription. The cytoprotective functions of these HSPs are essential for cell survival, and thus it is critical that inducible HSP gene expression be executed rapidly and efficiently. There is an interaction between heat shock factor 1 (HSF1) and symplekin, a protein known to form a complex with the polyadenylation factors CstF and CPSF. HSF1-symplekin complexes are detected only after stress treatment, and these two proteins co-localize in punctate nuclear structures in stressed cells (2). A chaperone/Hsp functioning as repressor of heat shock transcription factor (HSF) could make activation of hsp genes dependent on protein unfolding. It has been concluded that Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of HSF1 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HSF1