HSD3B1 Antibody (FDO66Q) - Azide and BSA Free Summary
Immunogen |
JEG Choriocarcinoma cells |
Localization |
Membrane |
Isotype |
IgG1 |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
HSD3B1 |
Purity |
Protein G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry/ Immunofluorescence 10-20 ug/ml
- Immunohistochemistry 10-20 ug/ml
- Immunohistochemistry-Frozen 10-20 ug/ml
- Immunohistochemistry-Paraffin 10-20 ug/ml
- Western Blot
|
Application Notes |
This HSD3B1 antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded sections and frozen sections. There has been negative feedback with the use of this antibody in Western blot. |
Reactivity Notes
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
Tris-Glycine, 0.15 M NaCl |
Preservative |
No Preservative |
Concentration |
1 mg/ml |
Purity |
Protein G purified |
Alternate Names for HSD3B1 Antibody (FDO66Q) - Azide and BSA Free
Background
3A-hydroxysteroid dehydrogenase (HSD3B) is a bifunctional enzyme from 3-beta-HSD family that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid as well as ketosteroids, and is essential for the biosynthesis of all classes of hormonal steroids. HSD3B catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosteron. HSD3B is highly expressed in placenta, skin, mammary tissues etc and localize to ER/mitochondrion membranes as single-pass membrane protein wherein it plays critical role in lipid metabolism as well as steroid biosynthesis pathways. Among its different isoforms, HSD3B1 is abundantly expressed in human placenta and is implicated progesterone biosynthesis for the maintenance of pregnancy. HSD3B1 polymorphism has been linked to prostate cancer susceptibility, an increased risk of benign prostate hyperplasia, and essential hypertension.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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FAQs for HSD3B1 Antibody (NBP2-80781) (0)
Secondary Antibodies
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