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HSD3B1 Antibody (FDO66Q) - Azide and BSA Free

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Immunohistochemistry: HSD3B1 Antibody (FDO66Q) - Azide and BSA Free [NBP2-80781] - Staining of placental villi. Image from the standard format of this antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Gt, Pm, Pm, ShSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
FDO66Q
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

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HSD3B1 Antibody (FDO66Q) - Azide and BSA Free Summary

Immunogen
JEG Choriocarcinoma cells
Localization
Membrane
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HSD3B1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 10-20 ug/ml
  • Immunohistochemistry 10-20 ug/ml
  • Immunohistochemistry-Frozen 10-20 ug/ml
  • Immunohistochemistry-Paraffin 10-20 ug/ml
  • Western Blot
Application Notes
This HSD3B1 antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded sections and frozen sections. There has been negative feedback with the use of this antibody in Western blot.

Reactivity Notes

Marmoset.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine, 0.15 M NaCl
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for HSD3B1 Antibody (FDO66Q) - Azide and BSA Free

  • 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
  • 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I
  • 3-beta-HSD I
  • 3BETAHSD
  • 3-beta-hydroxy-Delta(5)-steroid dehydrogenase
  • 3BH
  • delta-5-3-ketosteroid isomerase
  • EC 1.1.1
  • HSD3B
  • HSDB3
  • HSDB3A
  • hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
  • hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1
  • I
  • progesterone reductase
  • SDR11E1
  • short chain dehydrogenase/reductase family 11E, member 1,3-beta-hydroxy-5-ene steroid dehydrogenase
  • steroid Delta-isomerase
  • Trophoblast antigen FDO161G

Background

3A-hydroxysteroid dehydrogenase (HSD3B) is a bifunctional enzyme from 3-beta-HSD family that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid as well as ketosteroids, and is essential for the biosynthesis of all classes of hormonal steroids. HSD3B catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosteron. HSD3B is highly expressed in placenta, skin, mammary tissues etc and localize to ER/mitochondrion membranes as single-pass membrane protein wherein it plays critical role in lipid metabolism as well as steroid biosynthesis pathways. Among its different isoforms, HSD3B1 is abundantly expressed in human placenta and is implicated progesterone biosynthesis for the maintenance of pregnancy. HSD3B1 polymorphism has been linked to prostate cancer susceptibility, an increased risk of benign prostate hyperplasia, and essential hypertension.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HSD3B1