![Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (FOXA1/1514) [NBP2-54575] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with FOXA1 Monoclonal Antibody (FOXA1/1514).](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-FOXA1-1514-Immunohistochemistry-Paraffin-NBP2-54575-img0001.jpg "Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (FOXA1/1514) [NBP2-54575] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with FOXA1 Monoclonal Antibody (FOXA1/1514).")
| Reactivity | Hu, Rt(-)Species Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | FOXA1/1514 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53338). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant human HNF-3 alpha/FoxA1 protein fragment (aa372-472) (exact sequence is proprietary) (Uniprot: P55317 ) |
| Localization | Nuclear |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | FOXA1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistology (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 49 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for HNF-3 alpha/FoxA1 Antibody (NBP2-54575)Find related products by research area.
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| Gene Symbol | FOXA1 |
![Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (FOXA1/1514) [NBP2-54575] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with FOXA1 Monoclonal Antibody (FOXA1/1514).](http://images.novusbio.com/fullsize/HNF-3-alpha-FoxA1-Antibody-FOXA1-1514-Immunohistochemistry-Paraffin-NBP2-54575-img0002.jpg "Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (FOXA1/1514) [NBP2-54575] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with FOXA1 Monoclonal Antibody (FOXA1/1514).")
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