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HMGCL Antibody

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Western Blot: HMGCL Antibody [NBP2-16860] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: HMGCL Antibody [NBP2-16860] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: HMGCL Antibody [NBP2-16860] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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HMGCL Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human HMGCL. The exact sequence is proprietary.
Localization
Mitochondrion matrix
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HMGCL
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Chicken (80%), Rat (86%), Bovine (89%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for HMGCL Antibody

  • 3-hydroxy-3-methylglutaryl-CoA lyase
  • 3-hydroxymethyl-3-methylglutaryl-CoA lyase
  • EC 4.1.3.4
  • HLmitochondrial

Background

The protein encoded by this gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol HMGCL
Uniprot