HMGCL Antibodies
We offer HMGCL Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each HMGCL Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our HMGCL Antibodies can be used in a variety of model species: Bovine, Guinea Pig, Human, Mouse, Porcine, Rat, Zebrafish. Use the list below to choose the HMGCL Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our HMGCL polyclonal antibodies and browse our HMGCL monoclonal antibody catalog.
Alternate Names for HMGCL Antibodies
anti-HMGCL antibody, anti-3-hydroxy-3-methylglutaryl-CoA lyase antibody, anti-3-hydroxymethyl-3-methylglutaryl-CoA lyase antibody, anti-EC 4.1.3.4 antibody, anti-HLmitochondrial antibody
10 Results for "hmgcl" in Primary Antibodies
![Western Blot: HMGCL Antibody [NBP1-32767] - rat liver lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:10000.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Western-Blot-NBP1-32767-img0007.jpg "Western Blot: HMGCL Antibody [NBP1-32767] - rat liver lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:10000.")
![Immunocytochemistry/Immunofluorescence: HMGCL Antibody [NBP1-32767] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: HMGCL protein stained by HMGCL antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32767-img0008.jpg)
![Immunohistochemistry-Paraffin: HMGCL Antibody [NBP1-32767] - SW480 xenograft, using HMGCL antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Immunohistochemistry-Paraffin-NBP1-32767-img0005.jpg)
![Western Blot: HMGCL Antibody [NBP1-32767] - (30 ug of whole cell lysate) A: H1299 10% SDS PAGE; antibody diluted at 1:3000](http://images.novusbio.com/fullsize/HMGCL-Antibody-Western-Blot-NBP1-32767-img0001.jpg)
![Western Blot: HMGCL Antibody [NBP1-32767] - mouse liver lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:10000.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Western-Blot-NBP1-32767-img0006.jpg)
![Western Blot: HMGCL Antibody [NBP1-58026] - analysis of HMGCL in LPS+ATP treated bone marrrow derived macrophages using anti-HMGCL antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Western-Blot-NBP1-58026-img0005.jpg "Western Blot: HMGCL Antibody [NBP1-58026] - analysis of HMGCL in LPS+ATP treated bone marrrow derived macrophages using anti-HMGCL antibody. Image from verified customer review.")
![Western Blot: HMGCL Antibody [NBP1-58026] - Titration: 1.25ug/ml Positive Control: Human Liver.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Western-Blot-NBP1-58026-img0003.jpg)
![Western Blot: HMGCL Antibody [NBP1-83251] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/HMGCL-Antibody-Western-Blot-NBP1-83251-img0005.jpg "Western Blot: HMGCL Antibody [NBP1-83251] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HMGCL Antibody [NBP1-83251] - Staining in human liver and skin tissues using anti-HMGCL antibody. Corresponding HMGCL RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Immunohistochemistry-Paraffin-NBP1-83251-img0008.jpg)
![Immunohistochemistry-Paraffin: HMGCL Antibody [NBP1-83251] - Staining of human liver shows high expression.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Immunohistochemistry-Paraffin-NBP1-83251-img0006.jpg)
![Immunohistochemistry-Paraffin: HMGCL Antibody [NBP1-83251] - Staining of human skin shows low expression as expected.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Immunohistochemistry-Paraffin-NBP1-83251-img0007.jpg)
![Western Blot: HMGCL Antibody [NBP3-38500] - Western blot analysis of various lysates using HMGCL Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 15s.](http://images.novusbio.com/fullsize/nbp3-38500_rabbit-hmgcl-pab-912202414213749.jpg "Western Blot: HMGCL Antibody [NBP3-38500] - Western blot analysis of various lysates using HMGCL Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 15s.")
![Immunohistochemistry: HMGCL Antibody [NBP3-38500] - Immunohistochemistry analysis of paraffin-embedded Human kidney using HMGCL Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38500_rabbit-hmgcl-pab-91220241581219.jpg)
![Immunohistochemistry: HMGCL Antibody [NBP3-38500] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using HMGCL Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38500_rabbit-hmgcl-pab-912202415175215.jpg)
![Immunohistochemistry: HMGCL Antibody [NBP3-38500] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using HMGCL Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38500_rabbit-hmgcl-pab-912202415143641.jpg)
![Immunohistochemistry: HMGCL Antibody (22H11) [NBP3-26224] - Image of HMGCL Antibody (22H11) diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26224_rabbit-hmgcl-mab-22h11-2722024857337.jpg "Immunohistochemistry: HMGCL Antibody (22H11) [NBP3-26224] - Image of HMGCL Antibody (22H11) diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: HMGCL Antibody (22H11) [NBP3-26224] - Positive Western Blot detected in: Hela whole cell lysate, PC-3 whole cell lysate, HEK293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate. All lanes: HMGCL Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 35, 27, 21 kDa Observed band size: 35 kDa](http://images.novusbio.com/fullsize/nbp3-26224_rabbit-hmgcl-mab-22h11-2822024114099.jpg)
![Flow Cytometry: HMGCL Antibody (22H11) [NBP3-26224] - Overlay Peak curve showing Hela cells stained with HMGCL Antibody (22H11) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26224_rabbit-hmgcl-mab-22h11-282202414183658.jpg)
![Western Blot: HMGCL Antibody (4F4-D1) [H00003155-M01] - Analysis of HMGCL expression in transfected 293T cell line by HMGCL monoclonal antibody (M01), clone 4F4-D1.Lane 1: HMGCL transfected lysate(34.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HMGCL-Antibody-4F4-D1-Western-Blot-H00003155-M01-img0002.jpg "Western Blot: HMGCL Antibody (4F4-D1) [H00003155-M01] - Analysis of HMGCL expression in transfected 293T cell line by HMGCL monoclonal antibody (M01), clone 4F4-D1.Lane 1: HMGCL transfected lysate(34.4 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: HMGCL Antibody (4F4-D1) [H00003155-M01] - GCL monoclonal antibody (M01), clone 4F4-D1 Western Blot analysis of HMGCL expression in A-431 ( Cat # L015V1 ).](http://images.novusbio.com/fullsize/HMGCL-Antibody-4F4-D1-Western-Blot-H00003155-M01-img0001.jpg)
![Western Blot: HMGCL Antibody [NBP2-16860] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Western-Blot-NBP2-16860-img0001.jpg "Western Blot: HMGCL Antibody [NBP2-16860] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: HMGCL Antibody [NBP2-16860] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Immunohistochemistry-Paraffin-NBP2-16860-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HMGCL Antibody [NBP2-97971] - Immunofluorescence staining of HMGCL in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HMGCL polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97971-img0001.jpg "Immunocytochemistry/Immunofluorescence: HMGCL Antibody [NBP2-97971] - Immunofluorescence staining of HMGCL in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HMGCL polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.")
![Immunohistochemistry-Paraffin: HMGCL Antibody [NBP2-97971] - Immunochemical staining of human HMGCL in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Immunohistochemistry-Paraffin-NBP2-97971-img0003.jpg)
![Immunohistochemistry-Paraffin: HMGCL Antibody [NBP2-97971] - Immunochemical staining of human HMGCL in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Immunohistochemistry-Paraffin-NBP2-97971-img0002.jpg)
![Western Blot: HMGCL Antibody (S08-7H5) [NBP3-19654] - Western blot detection of HMGCL in Hela cell lysates using NBP3-19654 (1:1000 diluted). Predicted band size: 34kDa. Observed band size: 34kDa.](http://images.novusbio.com/fullsize/HMGCL-Antibody-S08-7H5-Western-Blot-NBP3-19654-img0001.jpg "Western Blot: HMGCL Antibody (S08-7H5) [NBP3-19654] - Western blot detection of HMGCL in Hela cell lysates using NBP3-19654 (1:1000 diluted). Predicted band size: 34kDa. Observed band size: 34kDa.")