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HMGCL Antibody

Images

 
Western Blot: HMGCL Antibody [NBP1-83251] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4
Orthogonal Strategies: Immunohistochemistry-Paraffin: HMGCL Antibody [NBP1-83251] - Staining in human liver and skin tissues using anti-HMGCL antibody. Corresponding HMGCL RNA-seq data are presented for the same ...read more
Immunohistochemistry-Paraffin: HMGCL Antibody [NBP1-83251] - Staining of human liver shows high expression.
Immunohistochemistry-Paraffin: HMGCL Antibody [NBP1-83251] - Staining of human skin shows low expression as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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HMGCL Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GLQNEKNIVSTPVKIKLIDMLSEAGLSVIETTSFVSPKWVPQMGDHTEVLKGIQKFPGINYPVLTPNLKGFEAAVAAGAKEVVIFGAASELFTKKNINCSIEESFQRFDAILKAAQSANISVRGYVSCALGCPYEGKISPAK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HMGCL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
HMGCL Protein (NBP1-83251PEP)
Publications
Read Publication using NBP1-83251.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (86%). Reactivity reported in scientific literature (PMID: 23082721)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HMGCL Antibody

  • 3-hydroxy-3-methylglutaryl-CoA lyase
  • 3-hydroxymethyl-3-methylglutaryl-CoA lyase
  • EC 4.1.3.4
  • HLmitochondrial

Background

The protein encoded by the HMGCL gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes thefinal step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associatedwith HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been foundfor this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HMGCL Antibody (NBP1-83251)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for HMGCL Antibody (NBP1-83251) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on HMGCL

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Bioinformatics

Gene Symbol HMGCL