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![Western Blot: HMGCL Antibody [NBP1-32767] - rat liver lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:10000.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Western-Blot-NBP1-32767-img0007.jpg "Western Blot: HMGCL Antibody [NBP1-32767] - rat liver lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:10000.")
![Immunocytochemistry/Immunofluorescence: HMGCL Antibody [NBP1-32767] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: HMGCL protein stained by HMGCL antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32767-img0008.jpg)
![Immunohistochemistry-Paraffin: HMGCL Antibody [NBP1-32767] - SW480 xenograft, using HMGCL antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Immunohistochemistry-Paraffin-NBP1-32767-img0005.jpg)
![Western Blot: HMGCL Antibody [NBP1-32767] - (30 ug of whole cell lysate) A: H1299 10% SDS PAGE; antibody diluted at 1:3000](http://images.novusbio.com/fullsize/HMGCL-Antibody-Western-Blot-NBP1-32767-img0001.jpg)
![Western Blot: HMGCL Antibody [NBP1-32767] - mouse liver lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:10000.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Western-Blot-NBP1-32767-img0006.jpg)
![Western Blot: HMGCL Antibody [NBP1-58026] - analysis of HMGCL in LPS+ATP treated bone marrrow derived macrophages using anti-HMGCL antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Western-Blot-NBP1-58026-img0005.jpg "Western Blot: HMGCL Antibody [NBP1-58026] - analysis of HMGCL in LPS+ATP treated bone marrrow derived macrophages using anti-HMGCL antibody. Image from verified customer review.")
![Western Blot: HMGCL Antibody [NBP1-58026] - Titration: 1.25ug/ml Positive Control: Human Liver.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Western-Blot-NBP1-58026-img0003.jpg)
![Western Blot: HMGCL Antibody [NBP1-83251] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/HMGCL-Antibody-Western-Blot-NBP1-83251-img0005.jpg "Western Blot: HMGCL Antibody [NBP1-83251] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HMGCL Antibody [NBP1-83251] - Staining in human liver and skin tissues using anti-HMGCL antibody. Corresponding HMGCL RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Immunohistochemistry-Paraffin-NBP1-83251-img0008.jpg)
![Immunohistochemistry-Paraffin: HMGCL Antibody [NBP1-83251] - Staining of human liver shows high expression.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Immunohistochemistry-Paraffin-NBP1-83251-img0006.jpg)
![Immunohistochemistry-Paraffin: HMGCL Antibody [NBP1-83251] - Staining of human skin shows low expression as expected.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Immunohistochemistry-Paraffin-NBP1-83251-img0007.jpg)
![Western Blot: HMGCL Antibody [NBP3-38500] - Western blot analysis of various lysates using HMGCL Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 15s.](http://images.novusbio.com/fullsize/nbp3-38500_rabbit-hmgcl-pab-912202414213749.jpg "Western Blot: HMGCL Antibody [NBP3-38500] - Western blot analysis of various lysates using HMGCL Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 15s.")
![Immunohistochemistry: HMGCL Antibody [NBP3-38500] - Immunohistochemistry analysis of paraffin-embedded Human kidney using HMGCL Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38500_rabbit-hmgcl-pab-91220241581219.jpg)
![Immunohistochemistry: HMGCL Antibody [NBP3-38500] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using HMGCL Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38500_rabbit-hmgcl-pab-912202415175215.jpg)
![Immunohistochemistry: HMGCL Antibody [NBP3-38500] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using HMGCL Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38500_rabbit-hmgcl-pab-912202415143641.jpg)
![Immunohistochemistry: HMGCL Antibody (22H11) [NBP3-26224] - Image of HMGCL Antibody (22H11) diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26224_rabbit-hmgcl-mab-22h11-2722024857337.jpg "Immunohistochemistry: HMGCL Antibody (22H11) [NBP3-26224] - Image of HMGCL Antibody (22H11) diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: HMGCL Antibody (22H11) [NBP3-26224] - Positive Western Blot detected in: Hela whole cell lysate, PC-3 whole cell lysate, HEK293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate. All lanes: HMGCL Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 35, 27, 21 kDa Observed band size: 35 kDa](http://images.novusbio.com/fullsize/nbp3-26224_rabbit-hmgcl-mab-22h11-2822024114099.jpg)
![Flow Cytometry: HMGCL Antibody (22H11) [NBP3-26224] - Overlay Peak curve showing Hela cells stained with HMGCL Antibody (22H11) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26224_rabbit-hmgcl-mab-22h11-282202414183658.jpg)
![Western Blot: HMGCL Antibody (4F4-D1) [H00003155-M01] - Analysis of HMGCL expression in transfected 293T cell line by HMGCL monoclonal antibody (M01), clone 4F4-D1.Lane 1: HMGCL transfected lysate(34.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HMGCL-Antibody-4F4-D1-Western-Blot-H00003155-M01-img0002.jpg "Western Blot: HMGCL Antibody (4F4-D1) [H00003155-M01] - Analysis of HMGCL expression in transfected 293T cell line by HMGCL monoclonal antibody (M01), clone 4F4-D1.Lane 1: HMGCL transfected lysate(34.4 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: HMGCL Antibody (4F4-D1) [H00003155-M01] - GCL monoclonal antibody (M01), clone 4F4-D1 Western Blot analysis of HMGCL expression in A-431 ( Cat # L015V1 ).](http://images.novusbio.com/fullsize/HMGCL-Antibody-4F4-D1-Western-Blot-H00003155-M01-img0001.jpg)
![Western Blot: HMGCL Antibody [NBP2-16860] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Western-Blot-NBP2-16860-img0001.jpg "Western Blot: HMGCL Antibody [NBP2-16860] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: HMGCL Antibody [NBP2-16860] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Immunohistochemistry-Paraffin-NBP2-16860-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HMGCL Antibody [NBP2-97971] - Immunofluorescence staining of HMGCL in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HMGCL polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97971-img0001.jpg "Immunocytochemistry/Immunofluorescence: HMGCL Antibody [NBP2-97971] - Immunofluorescence staining of HMGCL in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HMGCL polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.")
![Immunohistochemistry-Paraffin: HMGCL Antibody [NBP2-97971] - Immunochemical staining of human HMGCL in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Immunohistochemistry-Paraffin-NBP2-97971-img0003.jpg)
![Immunohistochemistry-Paraffin: HMGCL Antibody [NBP2-97971] - Immunochemical staining of human HMGCL in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/HMGCL-Antibody-Immunohistochemistry-Paraffin-NBP2-97971-img0002.jpg)
![Western Blot: HMGCL Overexpression Lysate (Adult Normal) [NBL1-11617] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HMGCL.](http://images.novusbio.com/fullsize/HMGCL-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11617-img0002.jpg "Western Blot: HMGCL Overexpression Lysate (Adult Normal) [NBL1-11617] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HMGCL.")
![Western Blot: HMGCL Antibody (S08-7H5) [NBP3-19654] - Western blot detection of HMGCL in Hela cell lysates using NBP3-19654 (1:1000 diluted). Predicted band size: 34kDa. Observed band size: 34kDa.](http://images.novusbio.com/fullsize/HMGCL-Antibody-S08-7H5-Western-Blot-NBP3-19654-img0001.jpg "Western Blot: HMGCL Antibody (S08-7H5) [NBP3-19654] - Western blot detection of HMGCL in Hela cell lysates using NBP3-19654 (1:1000 diluted). Predicted band size: 34kDa. Observed band size: 34kDa.")
![SDS-Page: Recombinant Human HMGCL Protein [NBP1-78844] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-HMGCL-Protein-SDS-Page-NBP1-78844-img0001.jpg "SDS-Page: Recombinant Human HMGCL Protein [NBP1-78844] - 15% SDS-PAGE (3ug)")
![SDS-Page: Recombinant Human HMGCL Protein [NBP2-60212] - 15 % SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-HMGCL-Protein-SDS-Page-NBP2-60212-img0001.jpg "SDS-Page: Recombinant Human HMGCL Protein [NBP2-60212] - 15 % SDS-PAGE (3ug)")