![Western Blot: HLCS Antibody (1E4) [NBP2-01859] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HLCS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HLCS.](http://images.novusbio.com/fullsize/HLCS-Antibody-1E4-Western-Blot-NBP2-01859-img0002.jpg "Western Blot: HLCS Antibody (1E4) [NBP2-01859] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HLCS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HLCS.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, IHC |
| Clone | OTI1E4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length human recombinant protein of human HLCS(NP_000402) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | HLCS |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 80.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
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![Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/16332.jpg)
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![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: HLCS Antibody (1E4) [NBP2-01859] Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HLCS mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HLCS-Antibody-1E4-Immunohistochemistry-Paraffin-NBP2-01859-img0004.jpg "Immunohistochemistry-Paraffin: HLCS Antibody (1E4) [NBP2-01859] Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HLCS mouse monoclonal antibody.")
![Flow Cytometry: HLCS Antibody (1E4) [NBP2-01859] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HLCS antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/HLCS-Antibody-1E4-Flow-Cytometry-NBP2-01859-img0001.jpg "Flow Cytometry: HLCS Antibody (1E4) [NBP2-01859] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HLCS antibody, and then analyzed by flow cytometry.")
![Immunohistochemistry-Paraffin: HLCS Antibody (1E4) [NBP2-01859] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HLCS mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HLCS-Antibody-1E4-Immunohistochemistry-Paraffin-NBP2-01859-img0003.jpg "Immunohistochemistry-Paraffin: HLCS Antibody (1E4) [NBP2-01859] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HLCS mouse monoclonal antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")