![Knockdown Validated: APCDD1 Antibody - BSA Free [NB110-92756] - APCDD1 knockdown potently (C) inhibited cell proliferation. Error bars represent mean +/- SD of three replicates (*P < 0.05, **P < 0.01, ***P < 0.001). (E) A673 and SKNMC cells stably expressing shMEIS1 were transiently transfected with plasmid encoding APCDD1, and subjected to immunoblotting and MTT assays. Bars represent mean +/- SD of three replicates (**P < 0.01). Image collected and cropped by CiteAb from the following publication (//academic.oup.com/nar/article/47/3/1255/5212009) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/APCDD1-Antibody---BSA-Free-Knockdown-Validated-NB110-92756-img0004.jpg "Knockdown Validated: APCDD1 Antibody - BSA Free [NB110-92756] - APCDD1 knockdown potently (C) inhibited cell proliferation. Error bars represent mean +/- SD of three replicates (*P < 0.05, **P < 0.01, ***P < 0.001). (E) A673 and SKNMC cells stably expressing shMEIS1 were transiently transfected with plasmid encoding APCDD1, and subjected to immunoblotting and MTT assays. Bars represent mean +/- SD of three replicates (**P < 0.01). Image collected and cropped by CiteAb from the following publication (//academic.oup.com/nar/article/47/3/1255/5212009) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, Ch, ReSpecies Glossary |
| Applications | WB, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.3 mg/ml |
| Immunogen | Synthetic peptide made to an internal portion of human APCDD1 (within residues 400-500). [Swiss-Prot# Q8J025] |
| Localization | Membrane. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | APCDD1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This APCDD1 antibody is useful for Western blot, where a band is seen ~58 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW | 58 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS and 30% Glycerol |
| Preservative | 0.1% Sodium Azide |
| Concentration | 1.3 mg/ml |
| Purity | Immunogen affinity purified |
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| Publications using NB110-92756 | Applications | Species |
|---|---|---|
| Ding W, Li X, Zhang J et al. Adaptive Functions of Structural Variants in Human Brain Development bioRxiv 2023-09-26 (Western Blot, Mouse) | Western Blot | Mouse |
| Lin L, Huang M, Shi X et al. Super-enhancer-associated MEIS1 promotes transcriptional dysregulation in Ewing sarcoma in co-operation with EWS-FLI1. Nucleic Acids Res. 2018-11-28 [PMID: 30496486] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for APCDD1 Antibody (NB110-92756)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | APCDD1 |
![Western Blot: APCDD1 Antibody [NB110-92756] - Detection of APCDD1 in human heart lysate](http://images.novusbio.com/fullsize/APCDD1-Antibody-Western-Blot-NB110-92756-img0003.jpg "Western Blot: APCDD1 Antibody [NB110-92756] - Detection of APCDD1 in human heart lysate")
![Western Blot: APCDD1 Antibody - BSA Free [NB110-92756] - APCDD1 is co-regulated by MEIS1 & EWS-FLI1 & mediates the oncogenic role of MEIS1. (A, B) Silencing of (A) MEIS1 or (B) EWS-FLI1 downregulated expression of both APCDD1 mRNA & protein. Error bars represent mean ± SD of three replicates (**P < 0.01, ***P < 0.001). (C, D) APCDD1 knockdown potently (C) inhibited cell proliferation & (D) decreased colony formation. Error bars represent mean ± SD of three replicates (*P < 0.05, **P < 0.01, ***P < 0.001). (E) A673 & SKNMC cells stably expressing shMEIS1 were transiently transfected with plasmid encoding APCDD1, & subjected to immunoblotting & MTT assays. Bars represent mean ± SD of three replicates (**P < 0.01). (F) Proposed model showing that MEIS1 & EWS-FLI1 co-operatively activate APCDD1 transcription, thereby promoting the malignant phenotype of Ewing sarcoma cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30496486), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-92756_rabbit-polyclonal-apcdd1-antibody-310202416205184.jpg "Western Blot: APCDD1 Antibody - BSA Free [NB110-92756] - APCDD1 is co-regulated by MEIS1 & EWS-FLI1 & mediates the oncogenic role of MEIS1. (A, B) Silencing of (A) MEIS1 or (B) EWS-FLI1 downregulated expression of both APCDD1 mRNA & protein. Error bars represent mean ± SD of three replicates (**P < 0.01, ***P < 0.001). (C, D) APCDD1 knockdown potently (C) inhibited cell proliferation & (D) decreased colony formation. Error bars represent mean ± SD of three replicates (*P < 0.05, **P < 0.01, ***P < 0.001). (E) A673 & SKNMC cells stably expressing shMEIS1 were transiently transfected with plasmid encoding APCDD1, & subjected to immunoblotting & MTT assays. Bars represent mean ± SD of three replicates (**P < 0.01). (F) Proposed model showing that MEIS1 & EWS-FLI1 co-operatively activate APCDD1 transcription, thereby promoting the malignant phenotype of Ewing sarcoma cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30496486), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: APCDD1 Antibody - BSA Free [NB110-92756] - APCDD1 is co-regulated by MEIS1 & EWS-FLI1 & mediates the oncogenic role of MEIS1. (A, B) Silencing of (A) MEIS1 or (B) EWS-FLI1 downregulated expression of both APCDD1 mRNA & protein. Error bars represent mean ± SD of three replicates (**P < 0.01, ***P < 0.001). (C, D) APCDD1 knockdown potently (C) inhibited cell proliferation & (D) decreased colony formation. Error bars represent mean ± SD of three replicates (*P < 0.05, **P < 0.01, ***P < 0.001). (E) A673 & SKNMC cells stably expressing shMEIS1 were transiently transfected with plasmid encoding APCDD1, & subjected to immunoblotting & MTT assays. Bars represent mean ± SD of three replicates (**P < 0.01). (F) Proposed model showing that MEIS1 & EWS-FLI1 co-operatively activate APCDD1 transcription, thereby promoting the malignant phenotype of Ewing sarcoma cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30496486), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-92756_rabbit-polyclonal-apcdd1-antibody-310202416212389.jpg "Western Blot: APCDD1 Antibody - BSA Free [NB110-92756] - APCDD1 is co-regulated by MEIS1 & EWS-FLI1 & mediates the oncogenic role of MEIS1. (A, B) Silencing of (A) MEIS1 or (B) EWS-FLI1 downregulated expression of both APCDD1 mRNA & protein. Error bars represent mean ± SD of three replicates (**P < 0.01, ***P < 0.001). (C, D) APCDD1 knockdown potently (C) inhibited cell proliferation & (D) decreased colony formation. Error bars represent mean ± SD of three replicates (*P < 0.05, **P < 0.01, ***P < 0.001). (E) A673 & SKNMC cells stably expressing shMEIS1 were transiently transfected with plasmid encoding APCDD1, & subjected to immunoblotting & MTT assays. Bars represent mean ± SD of three replicates (**P < 0.01). (F) Proposed model showing that MEIS1 & EWS-FLI1 co-operatively activate APCDD1 transcription, thereby promoting the malignant phenotype of Ewing sarcoma cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30496486), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")