Histone H3.3B Antibody Summary
Immunogen |
This antibody has been engineered to specifically recognize the recombinant protein Histone H3.3B using the following amino acid sequence: KPHRYRPGTVALREIRRYQKSTELLIR |
Predicted Species |
Mouse (100%), Rat (100%). Backed by our 100% Guarantee. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
H3F3B |
Purity |
Affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry/ Immunofluorescence 0.25-2 µg/ml
|
Application Notes |
For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS, pH 7.2, 40% glycerol |
Preservative |
0.02% Sodium Azide |
Purity |
Affinity purified |
Alternate Names for Histone H3.3B Antibody
Background
Histone H3.3B is 136 amino acids long, weighs approximately 15 kDa, and is part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Current studies are being done on several diseases and disorders including systemic lupus erythematosus, lupus erythematosus, malaria, leukemia, retinoblastoma, and immunodeficiency. Histone H3.3B has also been shown to have interactions with PELP1, HIST1H4A, HIST1H4B, HIST1H4C, and HIST1H4D in pathways such as the histone modification, development notch signaling, signal transduction for Activin A, amyloids, hemostasis, meiosis, meiotic recombination, and systemic lupus erythematosus pathways.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
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Secondary Antibodies
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