What is the difference in the following two HIF-1 alpha antibodies? Catalog # NB100-105 and NB100-134

NB100-105 is a mouse monoclonal antibody, whereas NB100-134 is a rabbit polyclonal. They both use the same immunogen.

I ordered this antibody but do not have a freezer to store it -20 degrees Celsius. Is it possible to store it for a short time (few days or weeks) at 4 degrees Celsius?

NB100-134 does contain Sodium Azide and should be fine at 4 degrees Celsius for about a week. We do recommend -20 degrees Celsius for storage and cannot guarantee this product will not degrade if kept at 4 degrees Celsius for more than a week.

I am going to do a Western blot to detect HIF-1a protein on HTR8/SVneo trophoblast cells. I have used NB100-134 HIF-1 a antibody for immunostaining and will use the same Ab for Western. Now my questions are 1. What should I use for blocking? 2. Can I use HIF-1 alpha recombinant protein (H00003091-P01) as positive control? 3. What can I use as loading control? 4. Can I use DAB as substrate or anything else?

1. You can use standard blocking solution which is 5% non-fat milk in TBST. 2. We recommend using Cos-7 Hypoxia Induced Nuclear Extract Kit (catalog #NB800-PC26) as the positive control for this antibody. 3. Since HIF-1 is a nuclear protein, you can use either alpha-Tubulin or beta-Tubulin as your loading control. 4. You can use DAB as your detection system.

Is the species reactivity of this antibody to Guinea pig based on computational homology?

The species reactivity reported for Guinea pig is based on published data. Here is the link for your reference . If you go through this paper carefully, on Figure 6, HIF1 expression has been tested across species including Cavia porcellus (Guinea pig).

Our customer would like to know the shelf life of your HIF-1 alpha Antibody (Cat. # NB100-134). We would appreciate if you can confirm this information for me as soon as possible. Moreover, I was wondering whether your antibody products have a general shelf life?

Unless otherwise stated on our website, our antibodies are guaranteed for 1 year from the date of receipt, whist peptides and proteins are guaranteed for three months from the date of receipt. Novus guarantees that our products will work in the applications and species listed on our website and on our product datasheets. If a Novus product fails to meet this criteria, our technical service team will work with you to troubleshoot the issue. If this process is not successful, Novus will provide you with a free replacement or a 100% refund for the product.

How much should I use for ChIP, 10E7 cells?

For ChIP, we recommend a dilution of 25 ul for 15 million cells. For 1 x 10^7 cells, you can use approximately 16-18 ul of this antibody.

Are there temperature requirements for storage?

We recommend short-term storage at 4C, and any long term storage at -20C. We recommend you aliquot any long-term storage and only remove what is needed for testing, as we recommend avoiding freeze-thaw cycles.

Does HIF1 alpha antibody (NB100-134) detect in the cytoplasm or the nucleus?

HIF1 alpha protein can be detected in both the cytoplasm and nucleus depending on the oxygen exposure of your tissues or cells. Cell that are more normoxic will have more signal in the cytoplasm while cells that are in hypoxic conditions will have a higher signal in the nucleus. Please see our publications for item NB100-134 for additional information regarding staining in IHC and ICC with this antibody.

Why is there a difference between the theoretical MW for HIF1A and the observed MW for HIF-1 alpha?

HIF1A, like many other proteins, has post-translational modifications. Depending on the size, amount and nature of the post-translational modifications, it can cause subtle to very large changes in molecular weight.

Which antibody(ies) do you recommend for the detection of HIF-1a by immunohistochemistry in the sections of paraffin-embedded mouse liver samples? I would appreciate if you can give me several choices and rank them in the order of performance. My goal is to distinguish HIF upregulation by prolyl hydroxylase inhibitor in different liver cells.

All of our antibodies are of high quality and are well tested/validated in species/applications we list on the datasheet. However, we suggest the following four HIF-1 alpha antibodies based upon customer reviews, as well as the number of peer reviewed publications in which these products have been cited by researchers from reputed institutes. (1) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-105) (cited in at least 218 peer reviewed publications) (2) HIF-1 alpha Antibody (cat# NB100-479) (cited in at least 51 peer reviewed publications) (3) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-123 ) (cited in at least 38 peer reviewed publications) (4) HIF-1 alpha Antibody (cat# NB100-449) (cited in at least 31 peer reviewed publications).

I would like to know, does a path exist for detection of HIF 1 in venous blood before and after revascularization of the leg?

We are not entirely sure if HIF-1 alpha will be present in the leg after revascularization. It may be present, but you may want to search the literature to see if this has been looked at before. If not, then this would certainly be an experiment worth doing.

What is the molecular weight (kDa) of protein HIF 1 alpha in western blot?

The theoretical molecular weight of HIF 1-alpha is ~93kDa. However, you will likely see a band between 100-120kDa due to phosphorylation.

We got the Hif1a (NB100-105) antibody from you guys. I used the concentration that is mentioned on your website, but I am getting a band of a completely different size (~70kDa) and not the 120 kDa mentioned.

HIF-1 alpha is a notoriously difficult protein to work with due to its rapid degradation. Therefore, the ~70kDa bands are most likely degradation products. It is very important to lyse the cells in hypoxic conditions. We strongly recommend lysing the cells directly into the Laemmli buffer and doing that quickly, so that the exposure to oxygen is minimized.Please go through our hypoxia related FAQs, you should find them very informative.Also, running a positive control may help confirm the band specificity in your samples. You may prepare them yourself or choose some from our catalog, for example: 1) HeLa Hypoxic / Normoxic Cell Lysate (NBP2-36452)2) HeLa Hypoxic (CoCl2) / Normoxic Cell Lysate (NBP2-36450)

I performed several Western Blots of HIF-1 alpha with different lysis buffers, whole lysates, and cytoplasm/nuclei extractions. I can’t seem to get a good western blot (poor signal, band much lower than expected, etc.). Can someone suggest some technical considerations/tricks I should consider using?

A major issue that researchers working with HIF-1 alpha is degradation due to exposure to oxygen. In western blot, this results in a weaker band and/or the appearance of multiple low molecular weight bands (40-80 kDa). We recommend preparing the lysates after collection of cells/tissues as quickly as possible (on ice), preferably in a hypoxic chamber. We also recommend including a true hypoxia mimetic control (eg: cells treated with CoCl2, DMOG… etc.). The controls help distinguish your band of interest from potential degradation/dimer bands.For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs.

I am doing HIF1 westerns in HIF-overexpressing mouse liver and adipose tissue using Novus antirabbit HIF1a antibody with overnight incubation. I am getting strong bands around 90kDa. I am aware that HIF theoretical molecular weight is 93kDa, but in westerns, the HIF band is usually around 120kDa according to my internet research. Can someone let me know if I’m getting the right HIF band or just some non-specific bands? Thanks.

(1) HIF-1 alpha’s theoretical molecular weight is 93kDa. The post translationally modified/ubiquitinated form of HIF-1 alpha protein (fails to undergo proteasomal degradation) shows up as a band in the 110-130 kDa range on a Western blot.(2) The dimeric protein may appear at a position above 200 kDa on non-reducing gels.(3) Importantly, HIFs are among the most rapidly degradable proteins; therefore, sample preparation is highly important when analyzing HIF1 alpha or HIF2 alpha. When degraded, HIF-1 alpha may show up between 40-80 kDa position on Western blot. Degradation may be avoided by preparing the samples as soon as possible after collection of cells/tissues in hypoxic chamber. Notably, the tissues/cells should be kept on ice during lysate preparation and the lysates should be analyzed as soon as possible.(4) For troubleshooting suggestions/feedback on more than 25 similar frequently asked questions, I would recommend visiting Novus page: FAQs - Hypoxia and HIFs (5) Last but not the least, Novus technical support team may be contacted via email

I have Hif1a nuclear protein extract at -80C. I am wondering if anyone knows how long it would be good for at that temperature since HIf1a is known to be degraded easily.Thank you!

You could try a few things to further inhibit the degradation.1) Use the protease inhibitors (if you are not already using them).2) Lyse cells into a buffer that contains SDS or LDS (eg: Laemmli's buffer), since SDS and LDS denature and inhibit proteases. Lysis may even be performed with reducing agents in the buffer (eg. DTT), but this will make your lysates unsuitable for BCA assay.3) Lysing samples rapidly ensures that the samples are instantly homogenized (it also shears DNA released by the SDS).5) Flash-freezing samples in liquid nitrogen rather than freezing at -80*C reduces the window of time for protease activity.6) Freeze samples in individual aliquots, instead of thawing the same vial multiple times.

I am curious to know the biochemical reactions of CoCl2 that mimic hypoxia. Is it that CoCl2 can bind any ubiquitin enzyme which regulates their degradation?

CoCl2 inhibits PHD enzymes (the body’s “oxygen sensors”) by replacing the Fe ion with Co, preventing these enzymes from marking HIF-1 alpha for degradation. CoCl2-based hypoxia mimetic samples are often used as positive control in HIF analysis. For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs.

I am curious to know the biochemical reactions of CoCl2 that mimic hypoxia. Is it that CoCl2 can bind any ubiquitin enzyme which regulates their degradation?

CoCl2 inhibits PHD enzymes (the body’s “oxygen sensors”) by replacing the Fe ion with Co, preventing these enzymes from marking HIF-1 alpha for degradation. CoCl2-based hypoxia mimetic samples are often used as positive control in HIF analysis. For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs.

Is cross-reactivity with HIF-2 alpha tested/predicted?

Although we don’t have cross-reactivity data with regards to HIF-2 alpha, we predict minimal cross-reactivity based on low sequence similarity observed from BLAST analysis between HIF-1 alpha and HIF-2 alpha.

We ordered and received the HIF-1 alpha antibody NB100-449 and on the packing slip it says that is prepared in TBS+0.1% BSA. I will be using it for western blots. DO you think my choice of blocking buffer (milk or Blotto) could interfere with the activity of this antibody or should it be necessarily BSA based?

Choice of blocking buffer is entirely within your discretion; it will not affect the antibody binding activity. Please note, some blocking buffers may work better than others and sometimes optimization is needed.When working with hypoxia there are other important factors to consider, as HIF-alpha is very easily degraded. The lysates should be freshly prepared. Also lysate preparation should be as quick as possible to avoid any exposure to oxygen - we recommend lysing cells directly into the SDS sample loading buffer (Laemmli buffer). We also highly recommend using positive control (you can prepare them yourself or choose some of those we have for sale).I have attached some additional information that you may find quite useful. Also here are some hypoxia related FAQ addressing common concerns.

Our lab recently ordered NB100-449, HIF-1 alpha antibody. Unfortunately an inexperienced technician stored it at -20C rather than 4C for approximately 2 days. Have you done any tests to determine antibody functionality if frozen?

The recommended storage condition of HIF-1 alpha antibody NB100-449 is 4C and we highly recommended not storing the product lower than the freezing point, as it may potentially disrupt the protein folding and destroy the antigen binding site of the antibody. Since we likely have not tested a storage condition of -20C for this antibody, we cannot really say if this antibody has been impaired by the storage condition. Our recommendation would be to test the antibody in a small portion of your treated cell line and see if the antibody is still reactive to the HIF-1 alpha protein.

Could you clarify if Yeast is a reactive species for HIF1a product #NB100-105?

This HIF-1 alpha antibody has not been tested in yeast. The homology is not significantly homologous so we do not believe there will be cross reactivity to the yeast protein.

Can HIF-1 alpha Antibody (Dylight 488), product NB100-479G, react with goat species? Does this product have preservatives in it?

NB100-479G has not been tested in goat species. Only the listed species on the product page and datasheet will be guaranteed. Reactivity: Hu, Mu, Rt, Ca, Fi, Ha, Pm, RbThe immunogen for this antibody corresponds to amino acids 530-825 of mouse Hifa. Running a sequence alignment of this sequence with the goat sequence found on UniProt yields around 81% homology.Mouse Hif1a: https://www.uniprot.org/uniprot/Q61221#sequencesGoat Hif1a: https://www.uniprot.org/uniprot/A0A023R978#sequencesLastly, there is 0.05% Sodium Azide present in the formulation of the product. This is also listed on the product page and datasheet. Buffer: 50mM Sodium BoratePreservative: 0.05% Sodium Azide

What's the difference between NBP2-75977 vs NBP2-75978?

While the same immunogen was used to make both HIF-1 alpha antibodies, they are different clones, meaning they recognize a different epitope on the immunogen .

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HIF-1 alpha Antibody - BSA Free

Name: HIF-1 alpha Antibody - BSA Free
Brand: Novus Biologicals
SKU: NB100-134
Rating: 4.8 (14 reviews)

(227 Publications)

(14 Reviews)

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Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - Analysis of HIF-1 alpha in human retinal and choroidal primary endothelia cells exposed to hypoxic conditions in the times noted using NB100-134. Image from ...read more

Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134] - Hif1alpha expression in neonatal and adult ovary. Section of P5 ovary showing MVH expression in all oocytes and Hif1alpha expression ...read more

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody - BSA Free [NB100-134] - Analysis of HIF-1 alpha in zebrafish retina tissue using NB100-134 HIF-1 alpha antibody. Mouse tissue was used as a control. Image from ...read more

Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - Analysis of HIF-1 alpha in overexpression and endogenous HIF-1 alpha & HIF-2 alpha using anti-HIF-1 alpha antibody NB100-134. The data showed that HIF-1 alpha ...read more

Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - Analysis of HIF-1 alpha using NB100-134, on normoxic and hypoxic nuclear rat cell lysates.

Biological Strategies: Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134] - Representative images of macrophages and activated macrophages, in the absence or presence of 10 uM ...read more

Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134] - Analysis of HIF-1 alpha in Normoxic (N) and Hypoxic (H) ARPE-19 cells using anti-HIF-1 alpha antibody NB100-134. Image from verified ...read more

Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody - BSA Free [NB100-134] - Hif1alpha expression in 9.5dpc primordial germ cells. Whole-mount staining of Hif1alpha and GFP in Oct4-GFP embryo (lateral view). ...read more

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody - BSA Free [NB100-134] - Staining in the canine CL on days 10 to 70 after ovulation. PC = positive control (human placenta). NC = negative control. Image from verified ...read more

Immunohistochemistry: HIF-1 alpha Antibody - BSA Free [NB100-134] - Staining of human kidney, renal tubular epithelium in cortex using NB100-134.

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody - BSA Free [NB100-134] - Analysis of FFPE tissue section of human endometrium carcinoma AN3CA cell line based xenograft using rabbit polyclonal HIF-1 alpha antibody ...read more

Simple Western: HIF-1 alpha Antibody - BSA Free [NB100-134] - Image shows a specific band for HIF-1 alpha in 0.2 mg/mL of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 ...read more

Immunoprecipitation: HIF-1 alpha Antibody - BSA Free [NB100-134] - PC-3 cell lysates run with cobalt chloride treatments. Immunoprecipitation was performed with protein A/G agarose beads. This data was provided courtesy ...read more

Dual RNAscope ISH-IHC: HIF-1 alpha Antibody - BSA Free [NB100-134] - Formalin-fixed paraffin-embedded tissue sections of human kidney were probed for HIF-1 alpha mRNA (ACD RNAScope Probe, catalog #605228; Fast Red ...read more

Simple Western: HIF-1 alpha Antibody - BSA Free [NB100-134] - Simple Western lane view shows lysates of MCF +/- DMOG, BioSpherix MSCs in hypoxic conditions, and BioSpherix MSCs in normoxic conditions loaded at 0.5 ...read more

P53 was increased response to IR in the fetal brain.Even though no significant change in protein level, total p53 and phosphorylation of P53 at ser15 were increased compared with HIF-1 alpha in fetal brains response to ...read more

WNT11 is induced by hypoxia or hypoxic mimetics in different cell types.(A) Increased Wnt11 mRNA in EMSC adipocytes (Day 12) after hypoxia-mimetic treatments. EMSC adipocytes were treated with CoCl2 (0.1 mM), DFO ...read more

Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - IGFBP5 inhibits HIF1 alpha expression through the MAPK-ERK signaling pathwayA. & B. Western blots using specific antibodies for the phosphorylation state of ...read more

Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - WNT11 regulates MMPs activities.(A–C) (Top panels): Serum-free medium was conditioned for 24 hrs by the indicated cells, concentrated 20-fold & assayed by ...read more

Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - ER-INP binds to PHD2, inhibits hydroxylation of HIF-1 alpha & increases HIF-1 alpha accumulation. (A) Co-immunoprecipitation assay. HEK293 cells were ...read more

Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - HIF-1 alpha binds to TIM-3 promoter & regulates its expression in primary glia.(a) Cell surface expression of TIM-3 analysed in BV2 cells under 20% O2 or 1% ...read more

Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - Regulation of catabolic genes by HIF-1 alpha . (A) Protein levels of Hif1a, & mRNA levels of Mmp13, & Hif2a in WT mouse primary chondrocytes transduced with ...read more

Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - NSCs Reduce Succinate Levels & Reprogram the Metabolism of Type 1 Pro-inflammatory Mφ toward Oxidative Phosphorylation In Vitro(A) Experimental setup for ...read more

Western Blot: HIF-1 alpha Antibody - BSA Free [NB100-134] - Enhanced expression of hypoxia-induced survival factors & angiogenic growth factors in hASC L-spheroids.(A) The light source used was LED (660 nm) designed to ...read more

Product Details

Summary

Reactivity	Hu, Mu, Rt, Bv, Ca, Gp, Pm, Xp, ZeSpecies Glossary
Applications	WB, Simple Western, ELISA, GS, ICC/IF, IHC, IP, WB, ChIP, Dual ISH-IHC, KO
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Format	BSA Free
Concentration	1.0 mg/ml

Validated by:

Biological Strategies

Order Details

View Available Conjugates

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB100-134AF350

Alexa Fluor 405 NB100-134AF405

Alexa Fluor 488 NB100-134AF488

Alexa Fluor 532 NB100-134AF532

Alexa Fluor 594 NB100-134AF594

Alexa Fluor 647 NB100-134AF647

Alexa Fluor 700 NB100-134AF700

Alexa Fluor 750 NB100-134AF750

Allophycocyanin NB100-134APC

Biotin NB100-134B

CoraFluor 1 NB100-134CL1

DyLight 350 NB100-134UV

DyLight 405 NB100-134V

DyLight 488 NB100-134G

DyLight 550 NB100-134R

DyLight 594 NB100-134DL594

DyLight 650 NB100-134C

DyLight 680 NB100-134FR

DyLight 755 NB100-134IR

FITC NB100-134F

HRP NB100-134H

Janelia Fluor 525 NB100-134JF525

Janelia Fluor 549 NB100-134JF549

Janelia Fluor 585 NB100-134JF585

Janelia Fluor 635 NB100-134JF635

Janelia Fluor 646 NB100-134JF646

Janelia Fluor 669 NB100-134JF669

PE NB100-134PE

PerCP NB100-134PCP

mFluor Violet 450 SE NB100-134MFV450

mFluor Violet 500 SE NB100-134MFV500

mFluor Violet 610 SE NB100-134MFV610

View Available Formulations

Catalog# & Formulation	Size	Price
BSA Free NB100-134SS

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Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-134AF350
Alexa Fluor 405	NB100-134AF405
Alexa Fluor 488	NB100-134AF488
Alexa Fluor 532	NB100-134AF532
Alexa Fluor 594	NB100-134AF594
Alexa Fluor 647	NB100-134AF647
Alexa Fluor 700	NB100-134AF700
Alexa Fluor 750	NB100-134AF750
Allophycocyanin	NB100-134APC
Biotin	NB100-134B
CoraFluor 1	NB100-134CL1
DyLight 350	NB100-134UV
DyLight 405	NB100-134V
DyLight 488	NB100-134G
DyLight 550	NB100-134R
DyLight 594	NB100-134DL594
DyLight 650	NB100-134C
DyLight 680	NB100-134FR
DyLight 755	NB100-134IR
FITC	NB100-134F
HRP	NB100-134H
Janelia Fluor 525	NB100-134JF525
Janelia Fluor 549	NB100-134JF549
Janelia Fluor 585	NB100-134JF585
Janelia Fluor 635	NB100-134JF635
Janelia Fluor 646	NB100-134JF646
Janelia Fluor 669	NB100-134JF669
PE	NB100-134PE
PerCP	NB100-134PCP
mFluor Violet 450 SE	NB100-134MFV450
mFluor Violet 500 SE	NB100-134MFV500
mFluor Violet 610 SE	NB100-134MFV610

HIF-1 alpha Antibody - BSA Free Summary

Immunogen	This HIF-1 alpha Antibody was developed against a fusion protein made to an internal sequence of human HIF-1 alpha (containing amino acids 432 - 528) [Uniprot# Q16665].
Localization	Nucleus
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	HIF1A
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions

Chromatin Immunoprecipitation (ChIP)
Chromatin Immunoprecipitation Sequencing reported in scientific literature (PMID 34277635)
Dual RNAscope ISH-IHC
ELISA
Gel Super Shift Assays 1:1 - 1:100
Immunoblotting
Immunocytochemistry/ Immunofluorescence
Immunohistochemistry 1:100 - 1:500
Immunohistochemistry-Frozen 1:100 - 1:500
Immunohistochemistry-Paraffin 1:100 - 1:500
Immunoprecipitation 1:1000
Knockout Validated
Simple Western 1:100
Western Blot 1:500 - 1:1000

Theoretical MW

93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Control

COS-7 Nuclear Hypoxic Induced Cell Lysate
Human Placenta Whole Tissue Lysate (Adult Whole Normal)
HeLa CoCl2 treated/Untreated Cell Lysate
HepG2 CoCl2 treated/Untreated Cell Lysate
HeLa Hypoxic / Normoxic Cell Lysate
HepG2 Hypoxic / Normoxic Cell Lysate
HIF-1 alpha Knockout CoCl2-treated/untreated HeLa Cell Lysate
HIF-1 alpha Knockout Hypoxic-treated/untreated HeLa Cell Lysate
NIH 3T3 CoCl2 treated/Untreated Cell Lysate
L929 CoCl2 treated/Untreated Cell Lysate
Raw 264.7 CoCl2 treated/Untreated Cell Lysate
PC12 CoCl2 treated/Untreated Cell Lysate
PC12 Hypoxic / Normoxic Cell Lysate
Raw 264.7 Hypoxic / Normoxic Cell Lysate
L929 Hypoxic / Normoxic Cell Lysate
NIH 3T3 Hypoxic / Normoxic Cell Lysate

Reviewed Applications

Read 14 Reviews rated 4.8

using
NB100-134 in the following applications:

Western Blot
Immunohistochemistry-Paraffin
Immunofluorescence
Immunoprecipitation

Publications

Read Publications using
NB100-134 in the following applications:

Block/Neutralize
2 publications
ChIP
3 publications
Chemotaxis
44 publications
Chip Cytometry
1 publication
ICC/IF
14 publications
IF/IHC
23 publications
IHC-Fr
5 publications
IHC-P
13 publications
IP
7 publications
Immunocytochemistry/ Immunofluorescence
1 publication
Immunohistochemistry
1 publication
Immunohistochemistry-Frozen
1 publication
Immunohistochemistry-Paraffin
1 publication
Immunoprecipitation
1 publication
In vivo assay
1 publication
Simple Western
1 publication
WB
97 publications
Western Blot
7 publications

Reactivity Notes

Use in Human reported in scientific literature (PMID:33654095).

Packaging, Storage & Formulations

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	0.02% Sodium Azide
Concentration	1.0 mg/ml
Purity	Immunogen affinity purified

Alternate Names for HIF-1 alpha Antibody - BSA Free

AINT
anti-HIF-1 alpha
anti-HIF1A
ARNT interacting protein
ARNT-interacting protein
Basic-helix-loop-helix-PAS protein MOP1
BHLHE78
Class E basic helix-loop-helix protein 78
HIF 1A
HIF1 alpha
HIF-1 alpha
HIF1
HIF1A
HIF-1a
HIF-1alpha
HIF-1-alpha
HIF1-alpha
hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
hypoxia-inducible factor 1-alpha
Member of PAS protein 1
member of PAS superfamily 1
MOP1
PAS domain-containing protein 8
PASD8

Background

Hypoxia contributes to the pathophysiology of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease (1). In cancer and particularly solid tumors, hypoxia plays a critical role in the regulation of genes involved in stem cell renewal, epithelial to mesenchymal transition (EMT), metastasis and angiogenesis. In the tumor microenvironment (TME), hypoxia influences the properties and function of stromal cells (e.g., fibroblasts, endothelial and immune cells) and is a strong determinant of tumor progression (2,3).

HIF-1 or hypoxia inducible factor 1 (predicted molecular weight 93kDa), is a transcription factor commonly referred to as a "master regulator of the hypoxic response" for its central role in the regulation of cellular adaptations to hypoxia. In its active form under hypoxic conditions, HIF-1 is stabilized by the formation of a heterodimer of HIF-1 alpha and ARNT/HIF-1 beta subunits. Nuclear HIF-1 engages p300/CBP for binding to hypoxic response elements (HREs). This process induces transcription and regulation of genes including EPO, VEGF, iNOS2, ANGPT1 and OCT4 (4,5).

Under normoxic conditions, the HIF-1 alpha subunit is rapidly targeted and degraded by the ubiquitin proteasome system. This process is mediated by prolyl hydroxylase domain enzymes (PHDs), which catalyze the hydroxylation of key proline residues (Pro-402 and Pro-564) within the oxygen-dependent degradation domain of HIF-1 alpha. Once hydroxylated, HIF-1 alpha binds the von Hippel-Lindau tumor suppressor protein (pVHL) for subsequent ubiquitination and proteasomal degradation (4). pVHL dependent regulation of HIF-1 alpha plays a role in normal physiology and disease states. Regulation of HIF-1 alpha by pVHL is critical for the suppressive function of FoxP3+ regulatory Tcells (6). Repression of pVHL expression in chronic lymphocytic leukemia (CLL) B cells leads to HIF-1 alpha stabilization and increased VEGF secretion (7).

References

1. Semenza, G. L., Agani, F., Feldser, D., Iyer, N., Kotch, L., Laughner, E., & Yu, A. (2000). Hypoxia, HIF-1, and the pathophysiology of common human diseases. Advances in Experimental Medicine and Biology.

2. Muz, B., de la Puente, P., Azab, F., & Azab, A. K. (2015). The role of hypoxia in cancer progression, angiogenesis, metastasis, and resistance to therapy. Hypoxia. https://doi.org/10.2147/hp.s93413

3. Huang, Y., Lin, D., & Taniguchi, C. M. (2017). Hypoxia inducible factor (HIF) in the tumor microenvironment: friend or foe? Science China Life Sciences. https://doi.org/10.1007/s11427-017-9178-y

4. Koyasu, S., Kobayashi, M., Goto, Y., Hiraoka, M., & Harada, H. (2018). Regulatory mechanisms of hypoxia-inducible factor 1 activity: Two decades of knowledge. Cancer Science. https://doi.org/10.1111/cas.13483

5. Dengler, V. L., Galbraith, M. D., & Espinosa, J. M. (2014). Transcriptional regulation by hypoxia inducible factors. Critical Reviews in Biochemistry and Molecular Biology. https://doi.org/10.3109/10409238.2013.838205

6. Lee, J. H., Elly, C., Park, Y., & Liu, Y. C. (2015). E3Ubiquitin Ligase VHL Regulates Hypoxia-Inducible Factor-1 alpha to Maintain Regulatory T Cell Stability and Suppressive Capacity. Immunity. https://doi.org/10.1016/j.immuni.2015.05.016

7. Ghosh, A. K., Shanafelt, T. D., Cimmino, A., Taccioli, C., Volinia, S., Liu, C. G., ... Kay, N. E. (2009). Aberrant regulation of pVHL levels by microRNA promotes the HIF/VEGF axis in CLL B cells. Blood. https://doi.org/10.1182/blood-2008-10-185686

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ARNT/HIF-1 beta
Antibody (H1beta234) ...

NB100-124

Immunocytochemistry/ Immunofluorescence ARNT/HIF-1 beta Antibody (H1beta234) - BSA Free

(4 Reviews)

(106 Publications)

Species: Bv, Ma, Hu, Mu, Pm, Rt, Sh

Applications: ChIP, CHIP-SEQ, GS, IB, ICC/IF, IHC, IHC-P, IP, WB

Erythropoietin/EPO
[HRP]

MEP00B

(105 Publications)

Species: Mu

Applications: ELISA

Von Hippel Lindau
Antibody (OTI1E1)

NBP2-45411

Species: Hu, Mu, Rt

Applications: IHC, IHC-P, WB

HIF-2 alpha/EPAS1
Antibody - BSA Free

NB100-122

Western Blot HIF-2 alpha/EPAS1 Antibody - BSA Free

(37 Reviews)

(823 Publications)

Species: Fi, Ha, Hu, Mu, Pm, Rb, Rt, Re, Sh

Applications: ChIP, Dual ISH-IHC, ELISA, Flow, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, KD, KO, PAGE, Simple Western, WB

AKT [p Ser473]
Antibody [Unconjugate ...

AF887

Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific

Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific

(49 Publications)

Species: Hu, Mu, Rt

Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB

Glut1 Antibody -
BSA Free

NB110-39113

Flow Cytometry Glut1 Antibody - BSA Free

(7 Reviews)

(62 Publications)

Species: Hu, Mu, Rb, Rt

Applications: ChIP, ChIP, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, Simple Western, WB

Carbonic Anhydrase
IX/CA9 Antibody - BSA ...

NB100-417

Immunoblotting Carbonic Anhydrase IX/CA9 Antibody - BSA Free

(7 Reviews)

(279 Publications)

Species: Hu, Mu, Pl, Rt

Applications: ChIP, Dual ISH-IHC, ELISA, Flow, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, MiAr, PLA, Simple Western, WB

p53 Antibody (PAb
240) - BSA Free

NB200-103

Flow (Intracellular) p53 Antibody (PAb 240) - BSA Free

(3 Reviews)

(42 Publications)

Species: Hu, Mu, Rt, Xp(-), Ye

Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB

ERK1 Antibody
(1E5) - BSA Free

NBP2-22203

Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free

Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free

(1 Publication)

Species: Hu, Pm, Mu, Rt

Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB

ERK2 Antibody
[Unconjugated]

AF1230

Immunohistochemistry ERK2 Antibody [Unconjugated]

Knockout Validated ERK2 Antibody [Unconjugated]

(3 Publications)

Species: Hu, Mu, Rt

Applications: IHC, KO, WB

TOR/mTOR [p
Ser2448] Antibody

NB600-607

Western Blot TOR/mTOR [p Ser2448] Antibody

(2 Reviews)

(9 Publications)

Species: Hu, Rt

Applications: ELISA, IHC, IHC-P, WB

HIF-1 alpha
Antibody (H1alpha67)

NB100-105

Flow Cytometry HIF-1 alpha Antibody (H1alpha67)

(45 Reviews)

(1040 Publications)

Species: Bv, Ca, Fe, Ma, Hu, Pm, Mu, Po, Pm, Rb, Rt, Sh, Xp

Applications: ChIP, ChIP, ELISA, Flow, GS, IA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, In vitro, KD, KO, LA, PLA, Simple Western, TCS, WB

iNOS Antibody -
BSA Free

NB300-605

(3 Reviews)

(130 Publications)

Species: Hu, Mu, Po, Rt

Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, WB

TIMP-1 [HRP]

DTM100

(97 Publications)

Species: Hu

Applications: ELISA

TNF-alpha
[Unconjugated]

210-TA

(3 Reviews)

(811 Publications)

Species: Hu

Applications: BA

EGLN1/PHD2 Antibody

NB100-137

Immunohistochemistry EGLN1/PHD2 Antibody

(12 Reviews)

(92 Publications)

Species: Hu, Mu, Pm, Rt

Applications: Flow, ICC/IF, IHC, IHC-P, IP, KD, KO, Simple Western, WB

Publications for HIF-1 alpha Antibody (NB100-134)(227)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Bovine, Canine, Chinese Hamster, Xenopus, Zebrafish.

We have publications tested in 18 applications: Block/Neutralize, ChIP, Chemotaxis, Chip Cytometry, ICC/IF, IF/IHC, IHC-Fr, IHC-P, IP, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, In vivo assay, Simple Western, WB, Western Blot.

Showing Publications 1 - 10 of 227. Show All 227 Publications.

Publications using NB100-134	Applications	Species
Ashutosh Singh, Donghang Cheng, Jyothishmathi Swaminathan, Yanwen Yang, Yan Zheng, Nancy Gordon, Vidya Gopalakrishnan REST-dependent downregulation of von Hippel-Lindau tumor suppressor promotes autophagy in SHH-medulloblastoma Scientific Reports 2024-06-12 [PMID: 38866867]
Nicole Verhaar, Anna Marei Grages, Fay J Sauer, Tobias Geiger, Wencke Reineking, Marion Hewicker-Trautwein, Florian Geburek, Sabine B R Kästner Measuring tissue oxygen saturation in the orad intestinal segment during equine colic surgery may aid in predicting the occurrence of postoperative ileus. American journal of veterinary research 2024-04-19 [PMID: 38626792]
Liao Q, Deng H, Wang Z et al. Deletion of prolyl hydroxylase domain-containing enzyme 3 (phd3) in zebrafish facilitates hypoxia tolerance Journal of Biological Chemistry 2023-11-01 [PMID: 37923141]
Do TC, Lau JW, Sun C et al. Hypoxia deactivates epigenetic feedbacks via enzyme-derived clicking proteolysis-targeting chimeras Science advances 2022-12-14 [PMID: 36516252] (WB, Zebrafish) Details: Dilution used in WB 1:1000	WB	Zebrafish
Oyama Y, Bartman CM, Bonney S et al. Circadian light-mediated endothelial metabolic reprogramming bioRxiv (Chemotaxis, Mouse)	Chemotaxis	Mouse
David A. Murphy, Daniela Osteicochea, Aidan Atkins, Caitlin Sannes, Zachary McClarnon, Isaac M. Adjei Optimizing Oxygen-Production Kinetics of Manganese Dioxide Nanoparticles Improves Hypoxia Reversal and Survival in Mice with Bone Metastases Molecular Pharmaceutics 2024-02-16 [PMID: 38365202]
Mücke, MM;El Bali, N;Schwarzkopf, KM;Uschner, FE;Kraus, N;Eberle, L;Mücke, VT;Bein, J;Beyer, S;Wild, PJ;Schierwagen, R;Klein, S;Zeuzem, S;Welsch, C;Trebicka, J;Brieger, A; The Role of Hypoxia-Inducible Factor 1 Alpha in Acute-on-Chronic Liver Failure International journal of molecular sciences 2024-01-26 [PMID: 38338821]
Wang T, Dong Y, Huang Z et al. Antioxidants stimulate BACH1-dependent tumor angiogenesis The Journal of clinical investigation 2023-08-31 [PMID: 37651203] (ChIP, Mouse)	ChIP	Mouse
Chen R, Wang H, Zheng C et al. Polo-like kinase 1 promotes pulmonary hypertension Respiratory Research 2023-08-19 [PMID: 37598171] (Western Blot)	Western Blot
Liao Q, Zhu C, Sun X et al. Disruption of sirtuin 7 in zebrafish facilitates hypoxia tolerance Journal of Biological Chemistry 2023-08-01 [PMID: 37481210] (In vivo assay, Block/Neutralize)	In vivo assay, Block/Neutralize
Lin L, Yang J, Zhang D et al. Vascular Endothelial Growth Factor A Contributes to Increased Mammalian Respiratory Epithelial Permeability Induced by Pasteurella multocida Infection Microbiology Spectrum 2023-04-13 [PMID: 36916939] (Western Blot)	Western Blot
Verhaar N, de Buhr N, von K�ckritz-Blickwede M et al. Hypoxia signaling in the equine small intestine: Expression and distribution of hypoxia inducible factors during experimental ischemia Frontiers in Veterinary Science 2023-02-24 [PMID: 36908508] (Immunohistochemistry)	Immunohistochemistry
Kishimoto H, Nakano T, Torisu K et al. Indoxyl sulfate induces left ventricular hypertrophy via the AhR-FGF23-FGFR4 signaling pathway Frontiers in cardiovascular medicine 2023-02-21 [PMID: 36895836] (WB, Rat)	WB	Rat
Lee E, Lee EA, Kong E et al. An agonistic anti-Tie2 antibody suppresses the normal-to-tumor vascular transition in the glioblastoma invasion zone Experimental & molecular medicine 2023-02-01 [PMID: 36828931] (IHC-P, Mouse)	IHC-P	Mouse
Barone FG, Urb� S, Clague MJ Segregation of pathways leading to pexophagy Life science alliance 2023-05-01 [PMID: 36810161] (WB, Human)	WB	Human
de Oliveira Freitas Machado C, Schafranek M, Br�ggemann M et al. Poison cassette exon splicing of SRSF6 regulates nuclear speckle dispersal and the response to hypoxia Nucleic acids research 2023-01-25 [PMID: 36620874] (WB, Human) Details: Dilution used 1:100	WB	Human
E G, Sun B, Liu B et al. Enhanced BPGM/2,3-DPG pathway activity suppresses glycolysis in hypoxic astrocytes via FIH-1 and TET2 Brain research bulletin 2022-11-08 [PMID: 36334804] (WB, Human) Details: Dilution used in WB 1:1000	WB	Human
La Salvia A, Carletti R, Verrico M et al. Angioside: The role of Angiogenesis and Hypoxia in Lung Neuroendocrine Tumours According to Primary Tumour Location in Left or Right Parenchyma Journal of clinical medicine 2022-10-09 [PMID: 36233825] (IHC-P, Human)	IHC-P	Human
Yang W, Wu W, Zhao Y et al. Caveolin-1 suppresses hippocampal neuron apoptosis via the regulation of HIF1? in hypoxia in naked mole-rats Cell Biology International 2022-12-01 [PMID: 36054154] (Immunoprecipitation)	Immunoprecipitation
Tikhonova MA, Maslov NA, Bashirzade AA et al. A Novel Laser-Based Zebrafish Model for Studying Traumatic Brain Injury and Its Molecular Targets Pharmaceutics 2022-08-22 [PMID: 36015377] (IHC-Fr, Zebrafish) Details: Dilution used 1:100	IHC-Fr	Zebrafish
Li X, Wang L, Qin X et al. Estrogens revert neutrophil hyperplasia by inhibiting Hif1 alpha-cMyb pathway in zebrafish myelodysplastic syndromes models Cell death discovery 2022-07-16 [PMID: 35842445] (IF/IHC, Zebrafish)	IF/IHC	Zebrafish
Madathilparambil S, Yalamanchili G, Rao T et al. Hypoxia Inducible Factor (HIF) 1 alpha induced regulation of lung injury in pulmonary aspiration is mediated through NF kappa B FASEB Bioadv 2022-05-06 [PMID: 35520392]
Vianello C, Cocetta V, Catanzaro D et al. Cisplatin resistance can be curtailed by blunting Bnip3-mediated mitochondrial autophagy Cell death & disease 2022-04-22 [PMID: 35459212] (WB, Human)	WB	Human
Cristancho AG, Gadra EC, Samba IM et al. Deficits in seizure threshold and other behaviors in adult mice without gross neuroanatomic injury after late gestation transient prenatal hypoxia Developmental neuroscience 2022-03-11 [PMID: 35279653] (WB)	WB
Fuentes-Fayos AC, P�rez-G�mez JM, G-Garc�a ME et al. SF3B1 inhibition disrupts malignancy and prolongs survival in glioblastoma patients through BCL2L1�splicing and mTOR/�-catenin pathways imbalances Journal of Experimental & Clinical Cancer Research 2022-12-01 [PMID: 35086552] (Western Blot)	Western Blot
Clees AS, Stolp V, Haupl B et al. Identification of the Cysteine Protease Legumain as a Potential Chronic Hypoxia-Specific Multiple Myeloma Target Gene Cells 2022-01-15 [PMID: 35053409]
Moberg M, AprO W, Horwath O et al. Acute normobaric hypoxia blunts contraction-mediated mTORC1- and JNK-signaling in human skeletal muscle Acta physiologica (Oxford, England) 2022-02-01 [PMID: 34984845] (WB, Human)	WB	Human
Lefort S, El-Naggar A, Tan S et al. De novo and cell line models of human mammary cell transformation reveal an essential role for Yb-1 in multiple stages of human breast cancer Cell death and differentiation 2021-07-22 [PMID: 34294889] (IHC-P)	IHC-P
Li W, Duan A, Xing Y Et Al. Transcription-Based Multidimensional Regulation of Fatty Acid Metabolism by HIF1 alpha in Renal Tubules Frontiers in cell and developmental biology 2021-07-02 [PMID: 34277635] (Chip Cytometry)	Chip Cytometry
Siddiqui F, Vukic V, Salminen T, Abankwa D Elaiophylin Is a Potent Hsp90/ Cdc37 Protein Interface Inhibitor with K-Ras Nanocluster Selectivity Biomolecules 2021-06-04 [PMID: 34199986] (Human)		Human
Wang RX, Henen MA, Lee JS et al. Microbiota-derived butyrate is an endogenous HIF prolyl hydroxylase inhibitor Gut Microbes 2021-06-30 [PMID: 34190032] (Western Blot, Block/Neutralize)	Western Blot, Block/Neutralize
Shao R, Zhang Z, Xu Z et al. H3K36 methyltransferase NSD1 regulates chondrocyte differentiation for skeletal development and fracture repair Bone research 2021-06-07 [PMID: 34099628] (WB, Mouse)	WB	Mouse
Lopez-Ramirez MA, Lai CC, Soliman SI et al. Astrocytes propel neurovascular dysfunction during cerebral cavernous malformation lesion formation The Journal of clinical investigation 2021-05-27 [PMID: 34043589]
Siddiqui FA, Parkkola H, Vukic V et al. Novel Small Molecule Hsp90/Cdc37 Interface Inhibitors Indirectly Target K-Ras-Signaling Cancers 2021-02-23 [PMID: 33672199] (WB, Human)	WB	Human
Katelyn J. McCann, Manoj Yadav, Mohammadali E. Alishahedani, Alexandra F. Freeman, Ian A. Myles, Pankaj K Singh Differential responses to folic acid in an established keloid fibroblast cell line are mediated by JAK1/2 and STAT3 PLoS ONE 2021-03-04 [PMID: 33662027]
Andrysik Z, Bender H, Galbraith MD, Espinosa JM Multi-omics analysis reveals contextual tumor suppressive and oncogenic gene modules within the acute hypoxic response Nature communications 2021-03-02 [PMID: 33654095] (Chemotaxis, Human)	Chemotaxis	Human
Perrin-Cocon L, Vidalain PO, Jacquemin C et al. A hexokinase isoenzyme switch in human liver cancer cells promotes lipogenesis and enhances innate immunity Communications biology 2021-02-16 [PMID: 33594203] (WB)	WB
Schaff DL, Singh S, Kim KB et al. Fragmentation of Small-Cell Lung Cancer Regulatory States in Heterotypic Microenvironments Cancer Research 2021-04-01 [PMID: 33531375] (Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen)	Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
Honda T, Hirakawa Y, Mizukami K et al. A distinctive distribution of hypoxia-inducible factor-1? in cultured renal tubular cells with hypoperfusion simulated by coverslip placement Physiological Reports 2021-01-01 [PMID: 33369883] (Western Blot)	Western Blot
Cheng YC, Chiang HY, Cheng SJ et al. Loss of the tumor suppressor BTG3 drives a pro-angiogenic tumor microenvironment through HIF-1 activation Cell death & disease 2020-12-11 [PMID: 33311481] (Chemotaxis, Human)	Chemotaxis	Human
Carrasco-Pozo, C, Tan, K N Et al. Hemin Prevents Increased Glycolysis in Macrophages upon Activation: Protection by Microbiota-Derived Metabolites of Polyphenols. Antioxidants (Basel) 2020-11-11 [PMID: 33187129] (IF/IHC, Mouse)	IF/IHC	Mouse
Turhan A, Pereira MT, Schuler G et al. Hypoxia-inducible factor (HIF1alpha) inhibition modulates cumulus cell function and affects bovine oocyte maturation in vitro Biol Reprod 2020-10-23 [PMID: 33095229] (WB, Bovine)	WB	Bovine
Dey A, Prabhudesai S, Zhang Y et al. Cystathione ?-synthase regulates HIF-1? stability through persulfidation of PHD2 Science Advances 2020-07-03 [PMID: 32937467] (Western Blot)	Western Blot
Yang W, Yu T, Huang X et al. Intestinal microbiota-derived short-chain fatty acids regulation of immune cell IL-22 production and gut immunity Nature communications 2020-09-08 [PMID: 32901017] (IP)	IP
Dowdell AS, Cartwright IM, Goldberg MS et al. The HIF target ATG9A is Essential for Epithelial Barrier Function and Tight Junction Biogenesis Mol. Biol. Cell 2020-07-29 [PMID: 32726170] (IP)	IP
Devraj, G, GuErit, S Et al. HIF-1 alpha is involved in blood-brain barrier dysfunction and paracellular migration of bacteria in pneumococcal meningitis. Acta Neuropathol 2020-08-01 [PMID: 32529267] (WB, IF/IHC, Human, Chinese Hamster)	WB, IF/IHC	Human, Chinese Hamster
Cohrs G, Drucks B, Synowitz M et al. Expression patterns of hypoxia inducible factors, pro-inflammatory, and neuroprotective cytokines in neuroepithelial tissues of lumbar spinal lipomas - a pilot study World Neurosurg 2020-06-06 [PMID: 32522652] (IF/IHC, Human)	IF/IHC	Human
James NE, Emerson JB, Borgstadt AD et al. The biomarker HE4 (WFDC2) promotes a pro-angiogenic and immunosuppressive tumor microenvironment via regulation of STAT3 target genes Sci Rep 2020-05-22 [PMID: 32444701] (WB, Human)	WB	Human
MEnEgaut L, Thomas C, Jalil A et al. Interplay between Liver X Receptor and Hypoxia Inducible Factor 1 alpha Potentiates Interleukin-1 beta Production in Human Macrophages Cell Rep 2020-01-01 [PMID: 32433974] (ICC/IF, Mouse)	ICC/IF	Mouse
Peng W, de Bruijn HS, Ten Hagen TLM et al. Targeted Photodynamic Therapy of Human Head and Neck Squamous Cell Carcinoma with Anti-epidermal Growth Factor Receptor Antibody Cetuximab and Photosensitizer IR700DX in the Mouse Skin-fold Window Chamber Model Photochem. Photobiol. 2020-03-29 [PMID: 32222965] (IF/IHC, Mouse)	IF/IHC	Mouse
Liu L, Zhang L, Zhao J et Al. Tumor Necrosis Factor Receptor-Associated Protein 1 Protects against Mitochondrial Injury by Preventing High Glucose-Induced mPTP Opening in Diabetes Oxid Med Cell Longev 2020-03-06 [PMID: 32215175]
Okada K, Mori D, Makii Y et Al. Hypoxia-inducible factor-1 alpha maintains mouse articular cartilage through suppression of NF- kappa B signaling Sci Rep 2020-03-25 [PMID: 32214220]
Lucia k. Studies on the role of hypoxia in growth hormone secreting pituitary tumor pathogenesis. Thesis. 1905-07-09 [PMID: 32111982] (Chemotaxis, IP, WB, IF/IHC, Human)	Chemotaxis, IP, WB, IF/IHC	Human
Lucia K, Wu Y, Garcia JM et al. Hypoxia and the hypoxia inducible factor 1 alpha activate protein kinase A by repressing RII beta subunit transcription Oncogene 2020-04-01 [PMID: 32111982] (IP, Chemotaxis, IF/IHC, WB, Rat)	IP, Chemotaxis, IF/IHC, WB	Rat
Bourdon R Regulation of epinephrine biosynthesis by intermittent and continuous hypoxia Nat Commun 2020-02-07 [PMID: 32024825]
Wierenga ATJ, Cunningham A, Erdem A et al. HIF1/2-exerted control over glycolytic gene expression is not functionally relevant for glycolysis in human leukemic stem/progenitor cells Cancer & Metabolism 2019-12-27 [PMID: 31890203]
Andrade FO, Nguyen NM, Warri A, Hilakivi-Clarke L Reversal of increased mammary tumorigenesis by valproic acid and hydralazine in offspring of dams fed high fat diet during pregnancy Sci Rep 2019-12-30 [PMID: 31889127] (WB, Mouse)	WB	Mouse
Chaboissier MC, Richardson N, Gillot I, Gregoire EP Roles of Id1/HIF-1 and CDK5/HIF-1 in cell cycle reentry induced by amyloid-beta peptide in post-mitotic cortical neuron Biochim Biophys Acta Mol Cell Res 2019-12-26 [PMID: 31884068] (WB, Rat)	WB	Rat
Zhao L, Zhao J, Gao R, et al. Intrabody against prolyl hydroxylase 2 ameliorates acetaminophen-induced acute liver injury in mice via concomitant promotion of angiogenesis and redox homeostasis Biomed. Pharmacother. 2019-12-16 [PMID: 31855737] (IHC-P, Mouse)	IHC-P	Mouse
Carrasco-Pozo C, Tan KN, Rodriguez T, Avery VM The Molecular Effects of Sulforaphane and Capsaicin on Metabolism upon Androgen and Tip60 Activation of Androgen Receptor Int J Mol Sci 2019-10-29 [PMID: 31671779] (ICC/IF, Human)	ICC/IF	Human
Zhao L, Liu Z, Yang F et al. Intrabody against prolyl hydroxylase 2 promotes angiogenesis by stabilizing hypoxia-inducible factor-1 alpha Sci Rep 2019-08-14 [PMID: 31413262] (WB, Human)	WB	Human
Satoh J, Asahina N, Kitano S. Bioinformatics data mining approach indicates the expression of chromatin immunoprecipitation followed by deep sequencing (ChIP-Seq)-based hypoxia-inducible factor-1alpha target genes in periplaque lesions of multiple sclerosis Clinical and Experimental Neuroimmunology. 2015-02-09 (Chemotaxis, Human)	Chemotaxis	Human
Gordijo CR, Abbasi AZ, Amini MA. Design of Hybrid MnO2-Polymer-Lipid Nanoparticles with Tunable Oxygen Generation Rates and Tumor Accumulation for Cancer Treatment Advanced Functional Materials. 2015-02-18 (IHC-P, Mouse)	IHC-P	Mouse
Han H, Jain AD, Truica MI et al. Small-Molecule MYC Inhibitors Suppress Tumor Growth and Enhance Immunotherapy Cancer Cell 2019-11-01 [PMID: 31679823]
Osawa T, Shimamura T, Saito K et al. Phosphoethanolamine Accumulation Protects Cancer Cells under Glutamine Starvation through Downregulation of PCYT2 Cell Reports 2019-10-01 [PMID: 31577958] (WB, Human)	WB	Human
Oyama Y, Bartman CM, Bonney S et al. Intense Light-Mediated Circadian Cardioprotection via Transcriptional Reprogramming of the Endothelium Cell Rep 2019-08-08 [PMID: 31390562] (Chemotaxis, Mouse)	Chemotaxis	Mouse
Mallikarjuna P, Raviprakash TS, Aripaka K et al. Interactions between TGF-beta type I receptor and hypoxia-inducible factor-alpha mediates a synergistic crosstalk leading to poor prognosis for patients with clear cell renal cell carcinoma Cell Cycle 2019-09-01 [PMID: 31339433] (WB, Human)	WB	Human
Masterson JC, Biette KA, Hammer JA et al. Epithelial HIF-1 alpha/claudin-1 axis regulates barrier dysfunction in eosinophilic esophagitis J. Clin. Invest. 2019-07-02 [PMID: 31264974] (Chemotaxis, Human)	Chemotaxis	Human
Abe H, Takeda N et al. Macrophage hypoxia signaling regulates cardiac fibrosis via Oncostatin M. Nat Commun 2019-06-27 [PMID: 31249305] (Chemotaxis, Mouse)	Chemotaxis	Mouse
Locri F, Dal Monte M, Aronsson M et al. UPARANT is an effective antiangiogenic agent in a mouse model of rubeosis iridis Journal of Molecular Medicine 2019-09-01 [PMID: 31243519] (Western Blot, Immunocytochemistry/ Immunofluorescence)	Western Blot, Immunocytochemistry/ Immunofluorescence
Kaushik DK, Bhattacharya A, Mirzaei R et al. Enhanced glycolytic metabolism supports transmigration of brain-infiltrating macrophages in multiple sclerosis J. Clin. Invest. 2019-05-21 [PMID: 31112527] (WB, Mouse)	WB	Mouse
Yang YD, Li MM, Xu G et al. Nogo-B Receptor Directs Mitochondria-Associated Membranes to Regulate Vascular Smooth Muscle Cell Proliferation Int J Mol Sci 2019-05-10 [PMID: 31083380] (ICC/IF, Rat)	ICC/IF	Rat
Tumova, S;Kerimi, A;Williamson, G; Long term treatment with quercetin in contrast to the sulfate and glucuronide conjugates affects HIF1 alpha stability and Nrf2 signaling in endothelial cells and leads to changes in glucose metabolism Free Radic. Biol. Med. 2019-04-25 [PMID: 31029788] (Simple Western, Human)	Simple Western	Human
Harms MJ, Li Q, Lee S et al. Mature Human White Adipocytes Cultured under Membranes Maintain Identity, Function, and Can Transdifferentiate into Brown-like Adipocytes Cell Rep 2019-04-02 [PMID: 30943403] (WB, Mouse)	WB	Mouse
Fu H, Ding X, Zhang W, Kang YJ. Profiling of nuclear copper-binding proteins under hypoxic condition Biometals 2019-02-09 [PMID: 30739301] (WB, Human)	WB	Human
Wu Z, Zhang W, Kang YJ. Copper affects the binding of HIF-1a to the critical motifs of its target genes Metallomics 2018-12-19 [PMID: 30566157] (WB, Human)	WB	Human
Cheng Y, Sun M, Chen L et al. Ten-Eleven Translocation Proteins Modulate the Response to Environmental Stress in Mice Cell Rep 2018-12-11 [PMID: 30540950] (WB, Mouse)	WB	Mouse
Lambertini E, Penolazzi L, Angelozzi M et al. Hypoxia Preconditioning of Human MSCs: a Direct Evidence of HIF-1a and Collagen Type XV Correlation Cell. Physiol. Biochem. 2018-12-07 [PMID: 30537732] (Chemotaxis, Human)	Chemotaxis	Human
Fuhrmann DC, Wittig I, Brune B. TMEM126B deficiency reduces mitochondrial SDH oxidation by LPS, attenuating HIF-1a stabilization and IL-1B expression Redox Biol 2018-10-19 [PMID: 30368040] (WB, Human)	WB	Human
Yang LQ, Chen M, Zhang JL et al. Hypoxia Delays Oligodendrocyte Progenitor Cell Migration and Myelin Formation by Suppressing Bmp2b Signaling in Larval Zebrafish. Front Cell Neurosci. 2018-10-04 [PMID: 30337858] (WB, Zebrafish)	WB	Zebrafish
Cohrs G, Drucks B, Surie JP et al. Expression profiles of pro-inflammatory and pro-apoptotic mediators in secondary tethered cord syndrome after myelomeningocele repair surgery. Childs Nerv Syst. 2018-10-03 [PMID: 30280214] (Human)		Human
Stoyanoff T, Rodriguez J, Todaro J et al. Erythropoietin attenuates LPS-induced microvascular damage in a murine model of septic acute kidney injury Biomedicine & Pharmacotherapy 2018-11-01 [PMID: 30257316] (WB, Mouse)	WB	Mouse
Ronellenfitsch MW, Zeiner PS, Mittelbronn M et al. Akt and mTORC1 signaling as predictive biomarkers for the EGFR antibody nimotuzumab in glioblastoma Acta Neuropathol Commun 2018-08-21 [PMID: 30129426] (IF/IHC, Human)	IF/IHC	Human
Zhang X, Chen L. Effects of CoCl2-simulated hypoxia on the expression levels of matrix metalloproteinases in renal adenocarcinoma cells and renal tubular epithelial cells Exp Ther Med Jun 21 2018 [PMID: 30116394] (WB, Human)	WB	Human
Zhang X, Chen L. Effects of CoCl2-simulated hypoxia on the expression levels of matrix metalloproteinases in renal adenocarcinoma cells and renal tubular epithelial cells Exp Ther Med Jun 21 2018 [PMID: 30116394] (WB, Human)	WB	Human
Zheng J, Zhang M, Weng H. Induction of the mitochondrial NDUFA4L2 protein by HIF-1a regulates heart regeneration by promoting the survival of cardiac stem cell. Biochem. Biophys. Res. Commun. 2018-06-28 [PMID: 29953852] (Chemotaxis, Human)	Chemotaxis	Human
Lefort S, El-Naggar A, Tan S et al. Identification of a Key Co-Operative Role of YB-1 in the Genesis and Metastasis of Human Breast Cancer bioRxiv (IF/IHC, Mouse, Human)	IF/IHC	Mouse, Human
McClendon J, Jansing NL, Redente EF et al. Hypoxia-Inducible Factor 1a Signaling Promotes Repair of the Alveolar Epithelium after Acute Lung Injury. Am. J. Pathol. 2017-08-01 [PMID: 28618253] (WB, Mouse)	WB	Mouse
Mimura I, Hirakawa Y, Kanki Y et al. Novel lnc RNA regulated by HIF-1 inhibits apoptotic cell death in the renal tubular epithelial cells under hypoxia. Physiol Rep. 2017-04-01 [PMID: 28420760] (Chemotaxis, Human)	Chemotaxis	Human
Kim O, Lee G, Kim K et al. 3-Hydroxymorphinan protects against hypoxia-induced cell death in primary astrocyte by regulating Ca2+ influx and the glutamate homeostasis Molecular & Cellular Toxicology 2022-07-27
Rennie MY. Development of the Fetoplacental Vascular Tree in Mice During Normal and Growth Restricted Pregnancies. 2011 University of Toronto. 2011-01-01
Chhipa RR, Fan Q, Anderson J et al. AMP kinase promotes glioblastoma bioenergetics and tumour growth. Nat. Cell Biol. 2018-07-01 [PMID: 29915361] (Chemotaxis, Human)	Chemotaxis	Human
Peruzzotti-Jametti L, Bernstock JD, Vicario N et al. Macrophage-Derived Extracellular Succinate Licenses Neural Stem Cells to Suppress Chronic Neuroinflammation. Cell Stem Cell. 2018-03-01 [PMID: 29478844] (WB)	WB
Dopeso H, Jiao HK, Cuesta AM et al. PHD3 Controls Lung Cancer Metastasis and Resistance to EGFR Inhibitors through TGFalpha. Cancer Res 2018-04-01 [PMID: 29339541]
Eriksson JA, Wanka C, Burger MC et al. Suppression of oxidative phosphorylation confers resistance against bevacizumab in experimental glioma J. Neurochem. 2017-11-27 [PMID: 29178334] (Human)		Human
Li D, Du Y, Yuan X et al. Hepatic hypoxia-inducible factors inhibit PPARalpha expression to exacerbate acetaminophen induced oxidative stress and hepatotoxicity. Free Radic. Biol. Med. 2017-06-03 [PMID: 28583670]
Zhang X, Liu L, Liu J et al. Endothelial cells co-cultured with renal carcinoma cells significantly reduce RECK expression under chemical hypoxia. Cell Biol. Int. 2017-05-31 [PMID: 28561419] (Human)		Human
Murakami A, Wang L, Kalhorn S et al. Context-dependent role for chromatin remodeling component PBRM1/BAF180 in clear cell renal cell carcinoma Oncogenesis 2017-01-16 [PMID: 28092369] (WB, Human)	WB	Human
Hou F, Li W, Shi Q et al. Yi Ai Fang, a traditional Chinese herbal formula, impacts the vasculogenic mimicry formation of human colorectal cancer through HIF-1alpha and epithelial mesenchymal transition. BMC Complement Altern Med. 2016-11-02 [PMID: 27806701] (WB, Human)	WB	Human
Kushida N, Nomura S, Mimura I et al. Hypoxia-Inducible Factor-1a Activates the Transforming Growth Factor-beta/SMAD3 Pathway in Kidney Tubular Epithelial Cells. Am. J. Nephrol. 2016-09-09 [PMID: 27607351]
Campillo N, Jorba I, Schaedel L et al. A Novel Chip for Cyclic Stretch and Intermittent Hypoxia Cell Exposures Mimicking Obstructive Sleep Apnea. Front Physiol. 2016-08-15 [PMID: 27524971] (ICC/IF, Rat)	ICC/IF	Rat
Sullivan AE, Peet DJ, Whitelaw ML. MAGED1 is a novel regulator of a select subset of bHLH PAS transcription factors. FEBS J. 2016-07-29 [PMID: 27472814] (WB)	WB
Kowitzke B, Cohrs G, Leuschner I et al. Cellular Profiles and Molecular Mediators of Lesion Cascades in the Placode in Human Open Spinal Neural Tube Defects. J Neuropathol Exp Neurol 2016-09-01 [PMID: 27354486] (IF/IHC, Human)	IF/IHC	Human
Hoang NT, Kadonosono T, Kuchimaru T, Kizaka-Kondoh S Hypoxia-inducible factor-targeting prodrug TOP3 combined with gemcitabine or TS-1 improves pancreatic cancer survival in an orthotopic model. Cancer Sci 2016-08-01 [PMID: 27270607] (WB)	WB
Salvi V, Vermi W, Gianello V et al. Dendritic cell-derived VEGF-A plays a role in inflammatory angiogenesis of human secondary lymphoid organs and is driven by the coordinated activation of multiple transcription factors. Oncotarget. 2016-06-28 [PMID: 27256980] (Chemotaxis, Human)	Chemotaxis	Human
Mammadzada P, Gudmundsson J, Kvanta A, Andre H. Differential hypoxic response of human choroidal and retinal endothelial cells proposes tissue heterogeneity of ocular angiogenesis Acta Ophthalmol. [PMID: 27255568] (WB, Human)	WB	Human
Spitzbarth I, Lempp C, Kegler K et al. Immunohistochemical and transcriptome analyses indicate complex breakdown of axonal transport mechanisms in canine distemper leukoencephalitis. Brain Behav. 2016-05-03 [PMID: 27247850] (IF/IHC, Canine)	IF/IHC	Canine
Jang JE, Ko MS, Yun JY et al. Nitric Oxide Produced by Macrophages Inhibits Adipocyte Differentiation and Promotes Profibrogenic Responses in Preadipocytes to Induce Adipose Tissue Fibrosis. Diabetes 2016-09-01 [PMID: 27246913] (WB, Mouse)	WB	Mouse
Semba H, Takeda N, Isagawa T et al. HIF-1alpha-PDk1 axis-induced active glycolysis plays an essential role in macrophage migratory capacity. Nat Commun. 2016-05-18 [PMID: 27189088] (Chemotaxis, Mouse)	Chemotaxis	Mouse
Takahashi N, Davy PM, Gardner LH et al. Hypoxia Inducible Factor 1 Alpha Is Expressed in Germ Cells throughout the Murine Life Cycle. PLoS ONE. 2016-05-06 [PMID: 27148974] (WB, Mouse)	WB	Mouse
Joyal JS, Sun Y, Gantner ML et al. Retinal lipid and glucose metabolism dictates angiogenesis through the lipid sensor Ffar1. Nat Med 2016-04-01 [PMID: 26974308]
Mori H, Yao Y, Learman BS et al. Induction of WNT11 by hypoxia and hypoxia-inducible factor-1alpha regulates cell proliferation, migration and invasion. Sci Rep. 2016-02-10 [PMID: 26861754] (WB, Human)	WB	Human
Anderson TMR. Integration of physiologic (HIF) and hormone (GR) stress signaling pathways in the regulation of breast tumor kinase (Brk/PTK6) expression in triple-negative breast. Thesis Jul 8 1905 [PMID: 26825173] (WB, Human)	WB	Human
Lin A, Li C, Xing Z et al. The LINK-A lncRNA activates normoxic HIF1alpha signalling in triple-negative breast cancer. Nat Cell Biol 2016-02-01 [PMID: 26751287] (WB)	WB
Song M, Liu T, Shi C et al. Bioconjugated Manganese Dioxide Nanoparticles Enhance Chemotherapy Response by Priming Tumor-Associated Macrophages toward M1-like Phenotype and Attenuating Tumor Hypoxia. ACS Nano. 2015-12-14 [PMID: 26650065] (IHC-Fr, Mouse)	IHC-Fr	Mouse
de Jong OG, van Balkom BW, Gremmels H, Verhaar MC. Exosomes from hypoxic endothelial cells have increased collagen crosslinking activity through up-regulation of lysyl oxidase-like 2. J. Cell. Mol. Med. 2015-11-27 [PMID: 26612622] (WB, Human)	WB	Human
Cheng S, Xing W, Pourteymoor S et al. Conditional Deletion of Prolyl Hydroxylase Domain-containing Protein 2 (Phd2) Gene Reveals its Essential Role in Chondrocyte Function and Endochondral Bone Formation. Endocrinology. 2015-11-12 [PMID: 26562260] (IHC-P, WB, Mouse)	IHC-P, WB	Mouse
Highet AR, Khoda SM, Buckberry S et al. Hypoxia induced HIF-1/HIF-2 activity alters trophoblast transcriptional regulation and promotes invasion. Eur. J. Cell Biol. 2015-12-01 [PMID: 26531845] (IHC-P, ICC/IF, Human) Details: HIF2 alpha antibody was used for IHC-P on early / late first trimester and term uncomplicated control placentas. The IHC assay involved antigen retrieval through boiling the slides with citrate buffer pH 6.5 in microwave, blocking of endogenous peroxidase using 6% H2O2 for 1 hour, use of primary at 1:1500 dilution, and detection using biotinylated secondary, Streptavidin-HRP conjugate-DAB followed by counterstaining with haematoxylin (Fig. 5).	IHC-P, ICC/IF	Human
Andre H, Tunik S, Aronsson M, Kvanta A. Hypoxia-Inducible Factor-1alpha Is Associated With Sprouting Angiogenesis in the Murine Laser-Induced Choroidal Neovascularization Model. ARVO Journals. 2015-10-01 [PMID: 26501235] (IF/IHC, Mouse)	IF/IHC	Mouse
Chen PJ, Weng JY, Hsu PH et al. NPGPx modulates CPEB2-controlled HIF-1alpha RNA translation in response to oxidative stress. Nucleic Acids Res 2015-10-30 [PMID: 26446990] (WB)	WB
Chen X, Liu J, He B et al. Vascular endothelial growth factor (VEGF) regulation by hypoxia inducible factor-1 alpha (HIF1A) starts and peaks during endometrial breakdown, not repair, in a mouse menstrual-like model Hum. Reprod. 2015-06-25 [PMID: 26113661] (Chemotaxis, WB, Mouse) Details: HIF1 alpha antibody was used at 1:100 dilution for IHC-P analysis of 5um paraffin sections of uteri tissues from mouse menstrual-like model (details in Fan et al Faseb J 2008 22 3571- 3580).	Chemotaxis, WB	Mouse
Park IS, Chung PS, Ahn JC. Enhancement of Ischemic Wound Healing by Spheroid Grafting of Human Adipose-Derived Stem Cells Treated with Low-Level Light Irradiation. PLoS One 2015-01-01 [PMID: 26065900] (ICC/IF, Human)	ICC/IF	Human
Wang J, Ding N, Li Y et al. Insulin-like growth factor binding protein 5 (IGFBP5) functions as a tumor suppressor in human melanoma cells. Oncotarget 2015-08-21 [PMID: 26010068] (WB, Human)	WB	Human
Zhang J, Han C, Dai H et al. Hypoxia-Inducible Factor-2a Limits Natural Killer T Cell Cytotoxicity in Renal Ischemia/Reperfusion Injury. J. Am. Soc. Nephrol. 2016-01-01 [PMID: 25956511] (WB, Mouse)	WB	Mouse
Forristal CE, Brown AL, Helwani FM et al. Hypoxia inducible factor (HIF)-2alpha accelerates disease progression in mouse models of leukemia and lymphoma but is not a poor prognosis factor in human AML. Leukemia. 2015-04-29 [PMID: 25921247] (WB, Mouse)	WB	Mouse
Saeedi BJ, Kao DJ, Kitzenberg DA et al. HIF-dependent regulation of claudin-1 is central to intestinal epithelial tight junction integrity Mol. Biol. Cell 2015-04-22 [PMID: 25904334] (Chemotaxis, Human)	Chemotaxis	Human
Koh HS, Chang CY, Jeon SB et al. The HIF-1/glial TIM-3 axis controls inflammation-associated brain damage under hypoxia. Nat Commun. 2015-03-20 [PMID: 25790768] (Chemotaxis, Mouse) Details: HIF-1 alpha antibody used for ChIP assay on mouse primary mixed glial cells that were incubated under hypoxia or normoxia for 24 hours then fixed with 1% formaldehyde/PBS (Figure 2e).	Chemotaxis	Mouse
Gang B, Rooke M, Sun R et al. Pyruvate dehydrogenase kinase expression profile is a biomarker for cancer sensitivity to dichloroacetate-mediated growth inhibition bioRxiv 2023-09-22 (WB, Human)	WB	Human
Garcia LN, Leimgruber C, Uribe Echevarria EM et al. Protective phenotypes of club cells and alveolar macrophages are favored as part of endotoxin-mediated prevention of asthma. Exp. Biol. Med. (Maywood). 2014-12-11 [PMID: 25504013] (WB, Mouse)	WB	Mouse
Hung YH, Chang SH, Huang CT et al. Inhibitor of Differentiation-1 and Hypoxia-Inducible Factor-1 Mediate Sonic Hedgehog Induction by Amyloid Beta-Peptide in Rat Cortical Neurons. Mol. Neurobiol. 2014-12-15 [PMID: 25502463] (WB, Rat)	WB	Rat
Sun KT, Chen MY, Tu MG et al. MicroRNA-20a regulates autophagy related protein-ATG16L1 in hypoxia-induced osteoclast differentiation. Bone. 2014-12-05 [PMID: 25485521] (Chemotaxis, Mouse) Details: p62/SQSTM1 antibody used for WB on RAW264.7 cells subjected to RANKL and M-CSF mediated osteoclast differentiation as well as hypoxic stress (Fig. 1E )	Chemotaxis	Mouse
Khamaisi M, Toukan H, Axelrod JH et al. Endothelin-converting enzyme is a plausible target gene for hypoxia-inducible factor. Kidney Int. 2014-12-03 [PMID: 25469848] (Chemotaxis, Human) Details: HIF-1 alpha antibody used for ChIP on HUVECs - cells cultured for 24 hours under normoxic/20% O2 or hypoxic/1% O2 conditions, cross-linked with 1% PFA for 10 min, neutrilized with 0.2 M glycine solution, recollected/suspended in SDS lysis buffer (10 mM Tris-HCl pH 8.0, 150 mM NaCl, 1% SDS, 1 mM EDTA), DNA-protein complex fragmented on Sonifier which followed immunoprecipitation with HIF-1 alpha antibody and magnetic beads - see full text for detailed protocol on ChIP-seq and ChIP-PCR analysis, and the results (Figure 6).	Chemotaxis	Human
Robertson ED, Wasylyk C, Ye T et al. The Oncogenic MicroRNA Hsa-miR-155-5p Targets the Transcription Factor ELK3 and Links It to the Hypoxia Response. PLoS One 2014-01-01 [PMID: 25401928] (WB, Human)	WB	Human
Dong Y, Ito T, Velayo C et al. Intrauterine Ischemic Reperfusion Switches the Fetal Transcriptional Pattern from HIF-1Alpha- to P53-Dependent Regulation in the Murine Brain. PLoS OnE. 2014-10-21 [PMID: 25329663] (Chemotaxis, WB, Mouse)	Chemotaxis, WB	Mouse
Oestreich KJ, Read KA, Gilbertson SE et al. Bcl-6 directly represses the gene program of the glycolysis pathway. Nat. Immunol. 2014-10-01 [PMID: 25194422] (Chemotaxis)	Chemotaxis
Li Xiaochen, Liu Xiansheng, Xu Yongjian et al. KLF5 promotes hypoxia-induced survival and inhibits apoptosis in non-small cell lung cancer cells via HIF-1alpha. Int J Oncol. 2014-07-17 [PMID: 25051115]
Li B, Qiu B, Lee DS et al. Fructose-1, 6-bisphosphatase opposes renal carcinoma progression. Nature 2014-09-11 [PMID: 25043030] (Chemotaxis, WB, IP, Human)	Chemotaxis, WB, IP	Human
Neary MT, Ng KE, Ludtmann MH et al. Hypoxia signaling controls postnatal changes in cardiac mitochondrial morphology and function. J. Mol. Cell. Cardiol. 2014-06-28 [PMID: 24984146] (Chemotaxis, Mouse) Details: ChIP was performed using 4 ug rabbit anti-mouse HIF1alpha antibody (NB100-134) as described in Breckenridge et al. PLoS Biol, 11 (2013), p. e1001666 in perinatal hearts of mice (Figure 4).	Chemotaxis	Mouse
Wang C, Cai Y, Zhang Y et al. Local Injection of Deferoxamine Improves Neovascularization in Ischemic Diabetic Random Flap by Increasing HIF-1alpha and VEGF Expression. PLoS ONE. 2014-06-26 [PMID: 24963878] (WB, Mouse) Details: Antibody used for WB in C57/bl6 male mice ischemic diabetic model. Figure 5 - WB of VEGF and HIF-1 alpha in diabetic flaps of deferoxamine (DFO) injected or not groups, 3 days after surgery; Figure 6 - WB of VEGF and HIF-1 alpha in dermal fibroblasts cultured in normoxia/21% O2 or hypoxia 1% O2 under different glucose concentrations in response to 72h DFO treatment .	WB	Mouse
Obama T, Takayanagi T, Kobayashi T et al. Vascular induction of a disintegrin and metalloprotease 17 by angiotensin II through hypoxia inducible factor 1alpha. Am J Hypertens 2015-01-01 [PMID: 24871629]
Nakamura A, Osonoi T, Terauchi Y. Relationship between urinary sodium excretion and pioglitazone-induced edema. J Diabetes Investig 2010-10-19 [PMID: 24843434]
Zecchini V, Madhu B, Russell R et al. Nuclear ARRB1 induces pseudohypoxia and cellular metabolism reprogramming in prostate cancer. EMBO J. 2014-05-16 [PMID: 24837709] (Chemotaxis, WB, ICC/IF, Human)	Chemotaxis, WB, ICC/IF	Human
Inoue T, Kohro T, Tanaka T et al. Cross-enhancement of ANGPTL4 transcription by HIF1 alpha and PPAR beta/delta is the result of the conformational proximity of two response elements. Genome Biol. 2014-04-22 [PMID: 24721177] (Chemotaxis, Human)	Chemotaxis	Human
Prasad P, Gordijo CR, Abbasi AZ et al. Multifunctional Albumin-MnO2 Nanoparticles Modulate Solid Tumor Microenvironment by Attenuating Hypoxia, Acidosis, Vascular Endothelial Growth Factor and Enhance Radiation Response. ACS Nano 2014-04-10 [PMID: 24702320] (IHC-P, Mouse)	IHC-P	Mouse
Li G, Ci W, Karmakar S et al. SPOP Promotes Tumorigenesis by Acting as a Key Regulatory Hub in Kidney Cancer. Cancer Cell 2014-03-18 [PMID: 24656772] (WB, Chemotaxis, Human)	WB, Chemotaxis	Human
Fahrenbach JP, Stoller D, Kim G et al. Abcc9 is required for the transition to oxidative metabolism in the newborn heart. FASEB J. 2014-03-19 [PMID: 24648545] (IHC-Fr, WB, Mouse)	IHC-Fr, WB	Mouse
Guo X, Dason ES, Zanon-Moreno V et al. PGC-1 alpha Signaling Coordinates Susceptibility to Metabolic and Oxidative Injury in the Inner Retina. Am. J. Pathol. 2014-02-28 [PMID: 24508229] (WB, Mouse)	WB	Mouse
Takeda K, Duan LJ, Takeda H. Improved vascular survival and growth in the mouse model of hindlimb ischemia by a remote signaling mechanism. Am. J. Pathol. 2014-02-18 [PMID: 24440788] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Glover LE, Bowers BE, Saeedi B et al. Control of creatine metabolism by HIF is an endogenous mechanism of barrier regulation in colitis. Proc Natl Acad Sci U S A 2013-12-03 [PMID: 24248342] (Chemotaxis, Human)	Chemotaxis	Human
Papa Pde C, Sousa LM, Silva Rdos S et al. Glucose transporter 1 expression accompanies hypoxia sensing in the cyclic canine corpus luteum. Reproduction. 2013-11-20 [PMID: 24140705] (WB, IF/IHC, Canine)	WB, IF/IHC	Canine
Tadokoro H, Umezu T, Ohyashiki K et al. Exosomes derived from hypoxic leukemia cells enhance tube formation in endothelial cells. J Biol Chem. 2013-11-29 [PMID: 24133215] (WB, Human)	WB	Human
Quenneville J, Feghaly A, Tual M et al. Long-term severe hypoxia adaptation induces non-canonical EMT and a novel Wilms Tumor 1 (WT1) isoform bioRxiv 2023-09-03 (ChIP, Human)	ChIP	Human
Gagnon E, Quenneville J, Feghaly A et al. Long-term severe hypoxia adaptation induces non-canonical EMT and a novel Wilms Tumor 1 (WT1) isoform. Research Square 2023-09-22 (ChIP, Human)	ChIP	Human
Pawlus MR, Wang L, Murakami A. STAT3 or USF2 Contributes to HIF Target Gene Specificity. PLoS One 2013-08-21 [PMID: 23991099] (Chemotaxis, Human) Details: Using the Biotin conjugated version of NB100-134, catalog number NB100-134B.	Chemotaxis	Human
Kanematsu S, Tanimoto K, Suzuki Y, Sugano S. Screening for possible miRNA-mRNA associations in a colon cancer cell line. Gene. 2013-08-09 [PMID: 23939471] (WB, Human)	WB	Human
Regan Anderson TM, Peacock DL, Daniel AR et al. Breast Tumor Kinase (Brk/PTK6) Is a Mediator of Hypoxia-Associated Breast Cancer Progression. Cancer Res. 2013-09-15 [PMID: 23928995] (WB, Human)	WB	Human
Sena JA, Wang L, Hu CJ. BRG1 and BRM chromatin-remodeling complexes regulate the hypoxia response by acting as coactivators for a subset of hypoxia-inducible transcription factor target genes. Mol Cell Biol 2013-10-01 [PMID: 23897427] (WB, Human)	WB	Human
Carbajo-Pescador S, Ordonez R, Benet M et al. Inhibition of VEGF expression through blockade of Hif1alpha and STAT3 signalling mediates the anti-angiogenic effect of melatonin in HepG2 liver cancer cells. Br J Cancer 2013-07-09 [PMID: 23756865] (Chemotaxis, ICC/IF, Human)	Chemotaxis, ICC/IF	Human
Pawlus MR, Wang L, Hu CJ. STAT3 and HIF1alpha cooperatively activate HIF1 target genes in MDA-MB-231 and RCC4 cells. Oncogene 2013-04-22 [PMID: 23604114] (Chemotaxis, Human)	Chemotaxis	Human
Shoji K, Murayama T, Mimura I et al. Sperm-Associated Antigen 4, a Novel Hypoxia-Inducible Factor 1 Target, Regulates Cytokinesis, and Its Expression Correlates with the Prognosis of Renal Cell Carcinoma. Am J Pathol 2013-04-17 [PMID: 23602831] (Chemotaxis, Human)	Chemotaxis	Human
Chu X, Gao X, Jansson L et al. Multiple Microvascular Alterations in Pancreatic Islets and Neuroendocrine Tumors of a Men1 Mouse Model. Am J Pathol 2013-04-11 [PMID: 23583653] (IHC-P, Mouse)	IHC-P	Mouse
Ganapathy S, Xiao S, Seo SJ et al. Low-dose arsenic induces chemotherapy protection via p53/NF-kB-mediated metabolic regulation Oncogene 2013-03-25 [PMID: 23524579] (ICC/IF, Human)	ICC/IF	Human
Kelly CJ, Glover LE, Campbell EL et al. Fundamental role for HIF-1alpha in constitutive expression of human beta defensin-1 Mucosal Immunol 2013-03-06 [PMID: 23462909] (Chemotaxis, Human)	Chemotaxis	Human
Bett JS, Ibrahim AF, Garg AK et al. The P-body component USP52/PAN2 is a novel regulator of HIF1A mRNA stability. Biochem J 2013-04-15 [PMID: 23398456] (WB, Human)	WB	Human
Yao W, Li J, Wang JJ et al. Effects of dietary ratio of n-6 to n-3 polyunsaturated fatty acids on immunoglobulins, cytokines, fatty acid composition, and performance of lactating sows and suckling piglets. J Anim Sci Biotechnol 2012-12-27 [PMID: 23270637] (IF/IHC, Human)	IF/IHC	Human
Enzmann G, Mysiorek C, Gorina R et al. The neurovascular unit as a selective barrier to polymorphonuclear granulocyte (PMN) infiltration into the brain after ischemic injury Acta Neuropathol 2012-12-27 [PMID: 23269317] (IF/IHC, Human)	IF/IHC	Human
Forristal CE, Winkler IG, Nowlan B et al. Pharmacologic stabilization of HIF-1alpha increases hematopoietic stem cell quiescence in vivo and accelerates blood recovery after severe irradiation. Blood 2013-01-31 [PMID: 23243286] (WB, Mouse)	WB	Mouse
Chiang CK, Nangaku M, Tanaka T et al. Endoplasmic reticulum (ER) stress signal impairs erythropoietin production: a role for ATF4 Am J Physiol Cell Physiol 2012-12-12 [PMID: 23242184] (Chemotaxis, WB, Human)	Chemotaxis, WB	Human
Yue Y, Zhang P, Liu D et al. Hypoxia preconditioning enhances the viability of ADSCs to increase the survival rate of ischemic skin flaps in rats. Aesthetic Plast Surg 2013-02-01 [PMID: 23232730] (WB, Rat)	WB	Rat
Eftedal I, Jorgensen A, Rosbjorgen R et al. Early genetic responses in rat vascular tissue after simulated diving Physiol Genomics 2012-11-06 [PMID: 23132759]
Hernandez-Luna MA, Rocha-Zavaleta L, Vega M, Huerta-Yepez S. HIF-1alpha induces a chemoresistance phenotype in a Non-Hodgkin's Lymphoma cell line via up-regulation of Bcl-xL Leuk Lymphoma 2012-09-27 [PMID: 23013270] (WB, IF/IHC, Human)	WB, IF/IHC	Human
Clambey ET, McNamee EN, Westrich JA et al. Hypoxia-inducible factor-1 alpha-dependent induction of FoxP3 drives regulatory T-cell abundance and function during inflammatory hypoxia of the mucosa Proc Natl Acad Sci U S A 2012-09-17 [PMID: 22988108] (Chemotaxis, Human)	Chemotaxis	Human
Pawlus MR, Wang L, Ware K, Hu CJ. USF2 and HIF2alpha cooperatively activate HIF2 target genes during hypoxia Mol Cell Biol 2012-09-10 [PMID: 22966206] (Chemotaxis, Human)	Chemotaxis	Human
Aguado-Fraile E, Ramos E, Saenz-Morales D et al. miR-127 Protects Proximal Tubule Cells against Ischemia/Reperfusion: Identification of Kinesin Family Member 3B as miR-127 Target PLoS One 2012-01-01 [PMID: 22962609] (Chemotaxis, Human)	Chemotaxis	Human
Morote-Garcia JC, Napiwotzky D, Kohler D, Rosenberger P. Endothelial Semaphorin 7A promotes neutrophil migration during hypoxia Proc Natl Acad Sci U S A 2012-08-13 [PMID: 22891341] (Chemotaxis, Human)	Chemotaxis	Human
Tuomela J, Sandholm J, Karihtala P et al. Low TLR9 expression defines an aggressive subtype of triple-negative breast cancer. Breast Cancer Res Treat. 2012-09-01 [PMID: 22847512] (WB, IF/IHC, Human)	WB, IF/IHC	Human
Vakili H, Jin Y, Cattini PA. Negative regulation of human growth hormone gene expression by insulin is dependent on hypoxia-inducible factor binding in primary non-tumor pituitary cells. J Biol Chem 2012-09-28 [PMID: 22833680] (WB)	WB
Mimura I, Nangaku M, Kanki Y et al. Dynamic change of chromatin conformation in response to hypoxia enhances the expression of GLUT3 (SLC2A3) by cooperative interaction of hypoxia-inducible factor 1 and KDM3A. Mol Cell Biol. 2012-08-01 [PMID: 22645302] (WB, Chemotaxis, Human)	WB, Chemotaxis	Human
Gang Yin, Ayesha B. Alvero, Vinicius Craveiro, Jennie C. Holmberg, Han-Hsuan Fu, Michele K. Montagna, Yang Yang, Ilana Chefetz-Menaker, Sudhakar Nuti, Michael Rossi, Dan-Arin Silasi, Thomas Rutherford, Gil Mor Constitutive Proteasomal Degradation of TWIST-1 in Epithelial Ovarian Cancer Stem Cells Impacts Differentiation and Metastatic Potential Oncogene 2013-05-24 [PMID: 22349827]
Copple BL, Kaska S, Wentling C. Hypoxia-inducible factor activation in myeloid cells contributes to the development of liver fibrosis in cholestatic mice. J Pharmacol Exp Ther. 2012-05-01 [PMID: 22271822] Details: IHC (frozen): Fig 1B, D (mouse liver). The Cre antibody (IMG-5720. IMG-5721 or IMG-5722) was used at 1:4000.
Rennie MY, Detmar J, Whiteley KJ et al. Expansion of the fetoplacental vasculature in late gestation is strain dependent in mice Am J Physiol Heart Circ Physiol 2012-03-01 [PMID: 22268107] (IF/IHC, WB, Mouse)	IF/IHC, WB	Mouse
Anderson S, Oyama TT, Lindsley JN et al. 2-Hydroxyestradiol slows progression of experimental polycystic kidney disease. Am J Physiol Renal Physiol. 2012-03-01 [PMID: 22160773] (WB, Rat)	WB	Rat
Tanimoto K, Tsuchihara K, Kanai A et al. Genome-wide identification and annotation of HIF-1alpha binding sites in two cell lines using massively parallel sequencing. Hugo J 2010-12-01 [PMID: 22132063] (Chemotaxis)	Chemotaxis
Ravichandran K, Tyagi A, Deep G et al. Interleukin-1beta-induced iNOS expression in human lung carcinoma A549 cells: involvement of STAT and MAPK pathways. Indian J Exp Biol 49(11):840-847. 2011-11-01 [PMID: 22126015]
Edrey Y, Casper D, Huchon D et al. Sustained high levels of neuregulin-1 in the longest-lived rodents; a key determinant of rodent longevity Aging Cell 2012-01-01 [PMID: 22103690] (WB, Mouse)	WB	Mouse
Bansal A, Harris RA, Degrado TR. Choline phosphorylation and regulation of transcription of choline kinase {alpha} in hypoxia. Journal of Lipid Research. 2011-10-24 [PMID: 22025560]
Farrow EG, Yu X, Summers LJ et al. Iron deficiency drives an autosomal dominant hypophosphatemic rickets (ADHR) phenotype in fibroblast growth factor-23 (Fgf23) knock-in mice. Proc Natl Acad Sci U S A 2011-11-15 [PMID: 22006328] (WB)	WB
Wang Y, Huang J-W, Li M et al. MicroRNA-138 modulates DNA damage response by repressing histone H2AX expression. Mol Cancer Res. 2011-06-21 [PMID: 21693595]
Lee SJ, Kim HP, Jin Y et al. Beclin 1 deficiency is associated with increased hypoxia-induced angiogenesis. Autophagy 2011-08-01 [PMID: 21685724] (Mouse)		Mouse
Raina S, Honer M, Kramer SD et al. Anti-VEGF treatment accelerates polycystic kidney disease. Am J Physiol Renal Physiol. 2011-06-15 [PMID: 21677148]
Gao W, Qiao X, Ma S, Cui L. Adipose-derived stem cells accelerate neovascularization in ischaemic diabetic skin flap via expression of hypoxia-inducible factor-1alpha. J Cell Mol Med 2011-12-01 [PMID: 21435171] (WB)	WB
Erman A, Wabitsch M, Goodyer CG. Human growth hormone receptor (GHR) expression in obesity: II. Regulation of the human GHR gene by obesity-related factors. Int J Obes (Lond) 2011-12-01 [PMID: 21386799] (IP, Human)	IP	Human
Belibi F, Zafar I, Ravichandran K et al. Hypoxia-inducible factor 1{alpha} (HIF-1 {alpha}) and autophagy in polycystic kidney disease (PKD). Am J Physiol Renal Physiol. 2011-01-01 [PMID: 21270095] (IHC-Fr, ICC/IF, WB, Mouse, Rat)	IHC-Fr, ICC/IF, WB	Mouse, Rat
Mazumdar Jolly, O'brien W Timothy, Johnson Randall S et al. O2 regulates stem cells through Wnt/B-catenin signalling. Nature Cell Biology 2010-01-01 [PMID: 20852629] (Chemotaxis, Mouse)	Chemotaxis	Mouse
Gubin MM, Calaluce R, Davis JW et al. Overexpression of the RNA binding protein HuR impairs tumor growth in triple negative breast cancer associated with deficient angiogenesis. Cell Cycle 2010-08-15 [PMID: 20724828] (IF/IHC, Mouse)	IF/IHC	Mouse
Dikow R, Schmidt U, Kihm LP et al. Uremia aggravates left ventricular remodeling after myocardial infarction. Am J Nephrol 2010-01-01 [PMID: 20484895] (IHC-P, Rat)	IHC-P	Rat
Filby CE, Hooper SB, Wallace MJ. Partial pulmonary embolization disrupts alveolarization in fetal sheep. Respir Res;11(1):42. 2010-04-23 [PMID: 20416033] (IHC-P, Human)	IHC-P	Human
Xia X, Kung AL. Preferential binding of HIF-1 to transcriptionally active loci determines cell-type specific response to hypoxia (BioMed Central). Genome Biol. 2009-01-01 [PMID: 19828020] (Chemotaxis, Human)	Chemotaxis	Human
Legendre C, Hori T, Loyer P et al. Drug-metabolising enzymes are down-regulated by hypoxia in differentiated human hepatoma HepaRG cells: HIF-1alpha involvement in CYP3A4 repression. Eur J Cancer 2009-11-01 [PMID: 19695866] (Human)		Human
Frede S, Stockmann C, Winning S et al. Hypoxia-inducible factor (HIF) 1alpha accumulation and HIF target gene expression are impaired after induction of endotoxin tolerance. J Immunol;182(10):6470-6. 2009-05-15 [PMID: 19414801] (WB, Human)	WB	Human
Rane S, He M, Sayed D et al. Downregulation of miR-199a derepresses hypoxia-inducible factor-1alpha and Sirtuin 1 and recapitulates hypoxia preconditioning in cardiac myocytes. Circ Res;104(7):879-86. 2009-04-10 [PMID: 19265035] (ICC/IF, Rat)	ICC/IF	Rat
Naito Y, Tsujino T, Matsumoto M et al. Adaptive response of the heart to long-term anemia induced by iron deficiency. Am J Physiol Heart Circ Physiol;296(3):H585-93. 2009-03-01 [PMID: 19136608] (WB, Rat)	WB	Rat
Botusan IR, Sunkari VG, Savu O et al. Stabilization of HIF-1alpha is critical to improve wound healing in diabetic mice. Proc Natl Acad Sci U S A;105(49):19426-31. 2008-12-09 [PMID: 19057015] (IF/IHC, WB, Mouse)	IF/IHC, WB	Mouse
Trollmann R1, Strasser K, Keller S et al. Placental HIFs as markers of cerebral hypoxic distress in fetal mice. Am J Physiol Regul Integr Comp Physiol. 2008-12-01 [PMID: 18832080] (WB, Mouse)	WB	Mouse
Meyuhas R, Pikarsky E, Tavor E et al. A Key role for cyclic AMP-responsive element binding protein in hypoxia-mediated activation of the angiogenesis factor CCN1 (CYR61) in Tumor cells. Mol Cancer Res;6(9):1397-409. 2008-09-01 [PMID: 18819928] (WB, Mouse)	WB	Mouse
Westfall SD, Sachdev S, Das P et al. Identification of oxygen-sensitive transcriptional programs in human embryonic stem cells. Stem Cells Dev 2008-10-01 [PMID: 18811242] (ICC/IF, Human)	ICC/IF	Human
Nikinmaa M, Leveelahti L, Dahl E et al. Population origin, development and temperature of development affect the amounts of HSP70, HSP90 and the putative hypoxia-inducible factor in the tadpoles of the common frog Rana temporaria. J Exp Biol;211(Pt 12):1999-2004. 2008-06-01 [PMID: 18515731] (WB, Xenopus)	WB	Xenopus
Lauzier, MC et al. (2R)-[(4-Biphenylylsulfonyl)amino]-N-hydroxy-3-phenylpropionamide (BiPS), a matrix metalloprotease inhibitor, is a novel and potent activator of hypoxia-inducible factors. Mol Pharmacol;74(1):282-8. 2008-07-01 [PMID: 18424552] (WB, Human)	WB	Human
Singh RP, Gu M, Agarwal R. Silibinin inhibits colorectal cancer growth by inhibiting tumor cell proliferation and angiogenesis. Cancer Res;68(6):2043-50. 2008-03-15 [PMID: 18339887] (IHC-P, Human)	IHC-P	Human
Zhang H, Bosch-Marce M, Shimoda LA et al. Mitochondrial autophagy is an HIF-1-dependent adaptive metabolic response to hypoxia. J Biol Chem;283(16):10892-903. 2008-04-18 [PMID: 18281291] (WB, Human)	WB	Human
Piantadosi CA, Suliman HB. Transcriptional Regulation of SDHa flavoprotein by nuclear respiratory factor-1 prevents pseudo-hypoxia in aerobic cardiac cells. J Biol Chem;283(16):10967-77. 2008-04-18 [PMID: 18252725] (IP, WB, ICC/IF, Human)	IP, WB, ICC/IF	Human
Sikder D, Kodadek T. The neurohormone orexin stimulates hypoxia-inducible factor-1 activity. Genes Dev;21(22):2995-3005. 2007-11-15 [PMID: 18006690] (WB, Human)	WB	Human
Ohtomo S, Nangaku M, Izuhara Y et al. Cobalt ameliorates renal injury in an obese, hypertensive type 2 diabetes rat model. Nephrol Dial Transplant;23(4):1166-72. 2008-04-01 [PMID: 17967803] (WB, IF/IHC, Rat)	WB, IF/IHC	Rat
Tao Y, Kim J, Yin Y et al. VEGF receptor inhibition slows the progression of polycystic kidney disease. Kidney Int 2007-12-01 [PMID: 17882148] (IF/IHC, Rat)	IF/IHC	Rat
Kim JW, Gao P, Liu YC et al. Hypoxia-inducible factor 1 and dysregulated c-Myc cooperatively induce vascular endothelial growth factor and metabolic switches hexokinase 2 and pyruvate dehydrogenase kinase 1. Mol Cell Biol;27(21):7381-93. 2007-11-01 [PMID: 17785433] (Chemotaxis, Human)	Chemotaxis	Human
Young HW, Williams OW, Chandra D et al. Central role of Muc5ac expression in mucous metaplasia and its regulation by conserved 5' elements. Am J Respir Cell Mol Biol;37(3):273-90. 2007-09-01 [PMID: 17463395] (Chemotaxis, Human)	Chemotaxis	Human
Harvey AJ, Kind KL, Thompson JG. Regulation of gene expression in bovine blastocysts in response to oxygen and the iron chelator desferrioxamine. Biol Reprod;77(1):93-101. 2007-07-01 [PMID: 17329595] (ICC/IF, Human)	ICC/IF	Human
Liao D, Corle C, Seagroves TN et al. Hypoxia-inducible factor-1alpha is a key regulator of metastasis in a transgenic model of cancer initiation and progression. Cancer Res 67(2):563-72. 2007-01-15 [PMID: 17234764] (WB, Human)	WB	Human
Pfafflin A, Brodbeck K, Heilig CW et al. Increased glucose uptake and metabolism in mesangial cells overexpressing glucose transporter 1 increases interleukin-6 and vascular endothelial growth factor production: role of AP-1 and HIF-1alpha. Cell Physiol Biochem 2006-01-01 [PMID: 17167225]
Lin Q, Lee YJ, Yun Z. Differentiation arrest by hypoxia. J Biol Chem;281(41):30678-83. 2006-10-13 [PMID: 16926163] (WB, Mouse)	WB	Mouse
Litz J, Krystal GW. Imatinib inhibits c-Kit-induced hypoxia-inducible factor-1alpha activity and vascular endothelial growth factor expression in small cell lung cancer cells. Mol Cancer Ther 2006-06-01 [PMID: 16818499] (WB, Human)	WB	Human
Wang J, Biju MP, Wang MH et al. Cytoprotective effects of hypoxia against cisplatin-induced tubular cell apoptosis: involvement of mitochondrial inhibition and p53 suppression. J Am Soc Nephrol;17(7):1875-85. 2006-07-01 [PMID: 16762987] (WB, Rat)	WB	Rat
Harris JR, Brown GA, Jorgensen M et al. Bone marrow-derived cells home to and regenerate retinal pigment epithelium after injury. Invest Ophthalmol Vis Sci;47(5):2108-13. 2006-05-01 [PMID: 16639022] (IHC-P, Mouse)	IHC-P	Mouse
LSC, Wilson SM. Redox regulation of lung development and perinatal lung epithelial function. Antioxid Redox Signal;7(1-2):92-107. 2005-01-01 [PMID: 15650399]
Liu X, Wu X, Cai L et al. Hypoxia preconditioning of cardiomyocytes cardioprotection: phophorylation of HIF-1 alpha induced by p42/p44 mitogen-activated protein kinases is involved. Pathophysiology;9(4):201-205. 2003-09-01 [PMID: 14567922] (WB, Rat)	WB	Rat
Zisman A, Pantuck AJ, Bui MH et al. LABAZ1: A metastatic tumor model for renal cell carcinoma expressing the carbonic anhydrase type 9 tumor antigen. Cancer Res 2003-08-15 [PMID: 12941820] (IF/IHC)	IF/IHC
Bergeron M, Gidday JM, Yu AY et al. Role of hypoxia-inducible factor-1 in hypoxia-induced ischemic tolerance in neonatal rat brain. Ann Neurol;48(3):285-96. 2000-09-01 [PMID: 10976634] (WB, Human)	WB	Human
Show All 227 Publications. Collapse Publications.

Reviews for HIF-1 alpha Antibody (NB100-134) (14) 4.814

Average Rating: 4.8

(Based on 14 reviews)

We have 14 reviews tested in 4 species: Human, Rat, Other, Zebrafish.

Reviews using NB100-134:

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IHC-P

(2)

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Human

(9)

Rat

(2)

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(2)

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reviewed by:
KARTHIKEYAN THIRUGNANAM

Zebrafish

02/07/2020

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Summary

Application	Western Blot
Sample Tested	Zebrafish embryo lysates
Species	Zebrafish
Lot	AP-13

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reviewed by:
Verified Customer

Human

10/28/2018

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Summary

Application	Western Blot
Sample Tested	bt549,Human MD-MB-231 cells
Species	Human
Lot	AE-7

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reviewed by:
Verified Customer

Human

04/30/2018

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Summary

Application	Western Blot
Sample Tested	20 ug whole cell lysate
Species	Human
Lot	AE-3

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reviewed by:
Verified Customer

Human

03/22/2017

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Summary

Application	Western Blot
Sample Tested	HCT116 whole cell lysate
Species	Human
Lot	AA2

Immunohistochemistry-Paraffin HIF-1 alpha NB100-134

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reviewed by:
Helder Andre

IHC-P

Other

08/02/2016

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Summary

Application	Immunohistochemistry-Paraffin
Sample Tested	Zebrafish retina with mouse tissue as control
Species	Other

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reviewed by:
Helder Andre

Human

07/07/2016

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Summary

Application	Western Blot
Sample Tested	Retinal and Choroidal primary endothelia
Species	Human

reviewed by:
Darren Richard

Rat

07/23/2015

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Summary

Application	Western Blot
Sample Tested	Vascular Smooth Muscle Cells
Species	Rat

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reviewed by:
Verified Customer

Human

06/01/2015

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Summary

Application	Western Blot
Sample Tested	MDA-MB-231 Cell Lysate
Species	Human

Immunofluorescence HIF-1 alpha NB100-134

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reviewed by:
Helder Andre

Human

05/14/2015

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Summary

Application	Immunofluorescence
Sample Tested	ARPE-19 cells
Species	Human

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reviewed by:
Xiuquan Luo

Human

09/18/2014

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Summary

Application	Western Blot
Sample Tested	HEK293 cell lysate
Species	Human

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reviewed by:
Pramod Mallikarjuna

Human

08/30/2014

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Summary

Application	Western Blot
Sample Tested	Clear cell renal cell carcinoma samples
Species	Human

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reviewed by:
Liza Sousa

IHC-P

Other

01/30/2014

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Summary

Application	Immunohistochemistry-Paraffin
Sample Tested	Canine corpus luteum
Species	Other

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reviewed by:
Sandhya Khurana

Rat

02/05/2012

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Summary

Application	Western Blot
Sample Tested	PC12 nuclear extract, Sample Amount: 50ug
Species	Rat

Immunoprecipitation HIF-1 alpha NB100-134

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reviewed by:
Kelie Reece

Human

10/27/2011

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Summary

Application	Immunoprecipitation
Sample Tested	PC-3 Cells
Species	Human
Lot	K3

Product General Protocols

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FAQs for HIF-1 alpha Antibody (NB100-134). (Showing 1 - 10 of 19 FAQs).

Why is there a difference between the theoretical MW for HIF1A and the observed MW for HIF-1 alpha?
- HIF1A, like many other proteins, has post-translational modifications. Depending on the size, amount and nature of the post-translational modifications, it can cause subtle to very large changes in molecular weight.
Which antibody(ies) do you recommend for the detection of HIF-1a by immunohistochemistry in the sections of paraffin-embedded mouse liver samples? I would appreciate if you can give me several choices and rank them in the order of performance. My goal is to distinguish HIF upregulation by prolyl hydroxylase inhibitor in different liver cells.
- All of our antibodies are of high quality and are well tested/validated in species/applications we list on the datasheet. However, we suggest the following four HIF-1 alpha antibodies based upon customer reviews, as well as the number of peer reviewed publications in which these products have been cited by researchers from reputed institutes. (1) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-105) (cited in at least 218 peer reviewed publications) (2) HIF-1 alpha Antibody (cat# NB100-479) (cited in at least 51 peer reviewed publications) (3) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-123 ) (cited in at least 38 peer reviewed publications) (4) HIF-1 alpha Antibody (cat# NB100-449) (cited in at least 31 peer reviewed publications).
I would like to know, does a path exist for detection of HIF 1 in venous blood before and after revascularization of the leg?
- We are not entirely sure if HIF-1 alpha will be present in the leg after revascularization. It may be present, but you may want to search the literature to see if this has been looked at before. If not, then this would certainly be an experiment worth doing.
What is the molecular weight (kDa) of protein HIF 1 alpha in western blot?
- The theoretical molecular weight of HIF 1-alpha is ~93kDa. However, you will likely see a band between 100-120kDa due to phosphorylation.
We got the Hif1a (NB100-105) antibody from you guys. I used the concentration that is mentioned on your website, but I am getting a band of a completely different size (~70kDa) and not the 120 kDa mentioned.
- HIF-1 alpha is a notoriously difficult protein to work with due to its rapid degradation. Therefore, the ~70kDa bands are most likely degradation products. It is very important to lyse the cells in hypoxic conditions. We strongly recommend lysing the cells directly into the Laemmli buffer and doing that quickly, so that the exposure to oxygen is minimized.Please go through our hypoxia related FAQs, you should find them very informative.Also, running a positive control may help confirm the band specificity in your samples. You may prepare them yourself or choose some from our catalog, for example: 1) HeLa Hypoxic / Normoxic Cell Lysate (NBP2-36452)2) HeLa Hypoxic (CoCl2) / Normoxic Cell Lysate (NBP2-36450)
I performed several Western Blots of HIF-1 alpha with different lysis buffers, whole lysates, and cytoplasm/nuclei extractions. I can’t seem to get a good western blot (poor signal, band much lower than expected, etc.). Can someone suggest some technical considerations/tricks I should consider using?
- A major issue that researchers working with HIF-1 alpha is degradation due to exposure to oxygen. In western blot, this results in a weaker band and/or the appearance of multiple low molecular weight bands (40-80 kDa). We recommend preparing the lysates after collection of cells/tissues as quickly as possible (on ice), preferably in a hypoxic chamber. We also recommend including a true hypoxia mimetic control (eg: cells treated with CoCl2, DMOG… etc.). The controls help distinguish your band of interest from potential degradation/dimer bands.For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs.
I am doing HIF1 westerns in HIF-overexpressing mouse liver and adipose tissue using Novus antirabbit HIF1a antibody with overnight incubation. I am getting strong bands around 90kDa. I am aware that HIF theoretical molecular weight is 93kDa, but in westerns, the HIF band is usually around 120kDa according to my internet research. Can someone let me know if I’m getting the right HIF band or just some non-specific bands? Thanks.
- (1) HIF-1 alpha’s theoretical molecular weight is 93kDa. The post translationally modified/ubiquitinated form of HIF-1 alpha protein (fails to undergo proteasomal degradation) shows up as a band in the 110-130 kDa range on a Western blot.(2) The dimeric protein may appear at a position above 200 kDa on non-reducing gels.(3) Importantly, HIFs are among the most rapidly degradable proteins; therefore, sample preparation is highly important when analyzing HIF1 alpha or HIF2 alpha. When degraded, HIF-1 alpha may show up between 40-80 kDa position on Western blot. Degradation may be avoided by preparing the samples as soon as possible after collection of cells/tissues in hypoxic chamber. Notably, the tissues/cells should be kept on ice during lysate preparation and the lysates should be analyzed as soon as possible.(4) For troubleshooting suggestions/feedback on more than 25 similar frequently asked questions, I would recommend visiting Novus page: FAQs - Hypoxia and HIFs (5) Last but not the least, Novus technical support team may be contacted via email
I have Hif1a nuclear protein extract at -80C. I am wondering if anyone knows how long it would be good for at that temperature since HIf1a is known to be degraded easily.Thank you!
- You could try a few things to further inhibit the degradation.1) Use the protease inhibitors (if you are not already using them).2) Lyse cells into a buffer that contains SDS or LDS (eg: Laemmli's buffer), since SDS and LDS denature and inhibit proteases. Lysis may even be performed with reducing agents in the buffer (eg. DTT), but this will make your lysates unsuitable for BCA assay.3) Lysing samples rapidly ensures that the samples are instantly homogenized (it also shears DNA released by the SDS).5) Flash-freezing samples in liquid nitrogen rather than freezing at -80*C reduces the window of time for protease activity.6) Freeze samples in individual aliquots, instead of thawing the same vial multiple times.
I am curious to know the biochemical reactions of CoCl2 that mimic hypoxia. Is it that CoCl2 can bind any ubiquitin enzyme which regulates their degradation?
- CoCl2 inhibits PHD enzymes (the body’s “oxygen sensors”) by replacing the Fe ion with Co, preventing these enzymes from marking HIF-1 alpha for degradation. CoCl2-based hypoxia mimetic samples are often used as positive control in HIF analysis. For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs.
I am curious to know the biochemical reactions of CoCl2 that mimic hypoxia. Is it that CoCl2 can bind any ubiquitin enzyme which regulates their degradation?
- CoCl2 inhibits PHD enzymes (the body’s “oxygen sensors”) by replacing the Fe ion with Co, preventing these enzymes from marking HIF-1 alpha for degradation. CoCl2-based hypoxia mimetic samples are often used as positive control in HIF analysis. For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs.
Is cross-reactivity with HIF-2 alpha tested/predicted?
- Although we don’t have cross-reactivity data with regards to HIF-2 alpha, we predict minimal cross-reactivity based on low sequence similarity observed from BLAST analysis between HIF-1 alpha and HIF-2 alpha.
What is the difference in the following two HIF-1 alpha antibodies? Catalog # NB100-105 and NB100-134
- NB100-105 is a mouse monoclonal antibody, whereas NB100-134 is a rabbit polyclonal. They both use the same immunogen.
I ordered this antibody but do not have a freezer to store it -20 degrees Celsius. Is it possible to store it for a short time (few days or weeks) at 4 degrees Celsius?
- NB100-134 does contain Sodium Azide and should be fine at 4 degrees Celsius for about a week. We do recommend -20 degrees Celsius for storage and cannot guarantee this product will not degrade if kept at 4 degrees Celsius for more than a week.
I am going to do a Western blot to detect HIF-1a protein on HTR8/SVneo trophoblast cells. I have used NB100-134 HIF-1 a antibody for immunostaining and will use the same Ab for Western. Now my questions are 1. What should I use for blocking? 2. Can I use HIF-1 alpha recombinant protein (H00003091-P01) as positive control? 3. What can I use as loading control? 4. Can I use DAB as substrate or anything else?
- 1. You can use standard blocking solution which is 5% non-fat milk in TBST. 2. We recommend using Cos-7 Hypoxia Induced Nuclear Extract Kit (catalog #NB800-PC26) as the positive control for this antibody. 3. Since HIF-1 is a nuclear protein, you can use either alpha-Tubulin or beta-Tubulin as your loading control. 4. You can use DAB as your detection system.
Is the species reactivity of this antibody to Guinea pig based on computational homology?
- The species reactivity reported for Guinea pig is based on published data. Here is the link for your reference. If you go through this paper carefully, on Figure 6, HIF1 expression has been tested across species including Cavia porcellus (Guinea pig).
Our customer would like to know the shelf life of your HIF-1 alpha Antibody (Cat. # NB100-134). We would appreciate if you can confirm this information for me as soon as possible. Moreover, I was wondering whether your antibody products have a general shelf life?
- Unless otherwise stated on our website, our antibodies are guaranteed for 1 year from the date of receipt, whist peptides and proteins are guaranteed for three months from the date of receipt. Novus guarantees that our products will work in the applications and species listed on our website and on our product datasheets. If a Novus product fails to meet this criteria, our technical service team will work with you to troubleshoot the issue. If this process is not successful, Novus will provide you with a free replacement or a 100% refund for the product.
How much should I use for ChIP, 10E7 cells?
- For ChIP, we recommend a dilution of 25 ul for 15 million cells. For 1 x 10^7 cells, you can use approximately 16-18 ul of this antibody.
Are there temperature requirements for storage?
- We recommend short-term storage at 4C, and any long term storage at -20C. We recommend you aliquot any long-term storage and only remove what is needed for testing, as we recommend avoiding freeze-thaw cycles.
Does HIF1 alpha antibody (NB100-134) detect in the cytoplasm or the nucleus?
- HIF1 alpha protein can be detected in both the cytoplasm and nucleus depending on the oxygen exposure of your tissues or cells. Cell that are more normoxic will have more signal in the cytoplasm while cells that are in hypoxic conditions will have a higher signal in the nucleus. Please see our publications for item NB100-134 for additional information regarding staining in IHC and ICC with this antibody.

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Control Lysate(s)

COS-7 Nuclear Hypoxic Induced Cell Lysate
Human Placenta Whole Tissue Lysate (Adult Whole Normal)
HeLa CoCl2 treated/Untreated Cell Lysate
HepG2 CoCl2 treated/Untreated Cell Lysate
HeLa Hypoxic / Normoxic Cell Lysate
HepG2 Hypoxic / Normoxic Cell Lysate
HIF-1 alpha Knockout CoCl2-treated/untreated HeLa Cell Lysate
HIF-1 alpha Knockout Hypoxic-treated/untreated HeLa Cell Lysate
NIH 3T3 CoCl2 treated/Untreated Cell Lysate
L929 CoCl2 treated/Untreated Cell Lysate
Raw 264.7 CoCl2 treated/Untreated Cell Lysate
PC12 CoCl2 treated/Untreated Cell Lysate
PC12 Hypoxic / Normoxic Cell Lysate
Raw 264.7 Hypoxic / Normoxic Cell Lysate
L929 Hypoxic / Normoxic Cell Lysate
NIH 3T3 Hypoxic / Normoxic Cell Lysate

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Blogs on HIF-1 alpha. Showing 1-10 of 37 blog posts - Show all blog posts.

Check out the latest blog posts on HIF-1 alpha.

Hypoxia-Dependent CAR Stabilizing Construct in T cells Improves Solid Tumor Targeting and Efficacy
By Victoria Osinski, PhDDespite advances in the development of cancer immunotherapies, those specifically targeting tumors still remains limited. Currently, there is great interest in utilizing chimeric antigen rece... Read full blog post.

Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ... Read full blog post.

Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases
Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff... Read full blog post.

Read full blog post.

Breast cancer stem cells survive chemotherapy through S100A10-ANXA2-SPT6 interaction that epigenetically promotes OCT4-mediated stemness
By Jamshed Arslan, Pharm D, PhDBreast cancer is the most common cancer among women that causes the greatest number of cancer-related deaths worldwide. After radiotherapy or cytotoxic chemotherapy like paclitax... Read full blog post.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein ... Read full blog post.

Bad news for stomach cancer: BAMBI protein inhibits gastric carcinoma via TGF-beta/epithelial-mesenchymal transition signaling
By Jamshed Arslan Pharm.D. Gastric carcinoma is the second leading cause of cancer-related deaths worldwide. One of the key features of gastric carcinoma is acidosis, which promotes growth and metastasis of gastric ... Read full blog post.

Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein
By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto... Read full blog post.

Stemness for Surviving Hypoxia: TGF-beta/Smad Signaling in Multiple Myeloma
By Jamshed Arslan Pharm.D. Multiple myeloma (MM) is a cancer of antibody-producing plasma cells. The bone marrow (BM) of MM patients is hypoxic, and MM cells overexpress many cancerous genes that are regulated by hy... Read full blog post.

Forecasting and Targeting a Rare Cancer with Hypoxia-Inducible Factor
By Jamshed Arslan Pharm.D. Cancers of nerve, adipose, and other soft tissues are called soft tissue sarcomas (STS). Malignant peripheral nerve sheath tumor (MPNST) is an example of a rare and hard-to-treat STS; eve... Read full blog post.

Showing 1-10 of 37 blog posts - Show all blog posts.

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Recent Reviews

4.8

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	KARTHIKEYAN THIRUGNANAM 02/07/2020
Application:	WB
Species:	Zebrafish

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	Verified Customer 10/28/2018
Application:	WB
Species:	Human

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	Verified Customer 04/30/2018
Application:	WB
Species:	Human

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Bioinformatics

Gene Symbol	HIF1A
Entrez	3091(Human) 15251(Mouse) 29560(Rat)
Uniprot	Q61221(Mouse) O35800(Rat)

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

HIF-1 alpha Antibody - BSA Free

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Reviews for HIF-1 alpha Antibody (NB100-134) (14) 4.814

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Summary

Summary

Summary

Summary

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FAQs for HIF-1 alpha Antibody (NB100-134). (Showing 1 - 10 of 19 FAQs).

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Blogs on HIF-1 alpha. Showing 1-10 of 37 blog posts - Show all blog posts.

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