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Human Placenta Whole Tissue Lysate (Adult Whole Normal)

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Western Blot: Human Placenta Whole Tissue Lysate (Adult Whole Normal) [NB820-59248] - Used as a positive control for human PiT-1 (SLC20A1) protein on an anti-human PiT-1 Western blot using human plancenta lysate. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
5.0 mg/ml

Order Details

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Human Placenta Whole Tissue Lysate (Adult Whole Normal) Summary

Description
We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.
Specificity
Total Protein - Human Adult Normal Tissue: Placenta

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Western Blot Electrophoresis
Reviewed Applications
Read 1 Review rated 5
using
NB820-59248 in the following application:

Publications
Read Publication using NB820-59248.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
Concentration
5.0 mg/ml

Lysate Details for Array

Type
Tissue
Life Stage
Adult
Tissue
Reproductive
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for Placenta Lysate (NB820-59248)(1)

We have publications tested in 1 application: WB.


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(1)
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Review for Placenta Lysate (NB820-59248) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NB820-59248:
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Images Ratings Applications Species Date Details
 Placenta NB820-59248
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5
reviewed by:
Elizabeth Soberg
06/13/2018
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Summary

LotN/A on datasheet

Comments

CommentsUsed as a positive control for human PiT-1 (SLC20A1) protein on an anti-human PiT-1 Western blot.
Used primary @ 1:1000
Used secondary @ 1:5000
Used entry level (ug sensitivity) ECL reagents

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Placenta Lysate (NB820-59248) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Placenta Products

Array NB820-59248

Blogs on Placenta

There are no specific blogs for Placenta, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

Calculators

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Recent Reviews

5
5
1
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1
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Elizabeth Soberg
06/13/2018
Application:
Species: