![Western Blot: Hexokinase 1 Antibody (4D7) [NBP1-04295] - The Recombinant Proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Hexokinase 1 recombinant protein Lane 2.: Hexokinase 2 recombinant protein Lane 3.: Hexokinase 3 recombinant protein Lane 4.: Hexokinase 4 recombinant protein](http://images.novusbio.com/fullsize/Hexokinase-1-Antibody-4D7-Western-Blot-NBP1-04295-img0011.jpg "Western Blot: Hexokinase 1 Antibody (4D7) [NBP1-04295] - The Recombinant Proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Hexokinase 1 recombinant protein Lane 2.: Hexokinase 2 recombinant protein Lane 3.: Hexokinase 3 recombinant protein Lane 4.: Hexokinase 4 recombinant protein")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 4D7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Recombinant human Hexokinase1 (1-917aa) purified from E. coli |
| Specificity | The antibody recognizes all four isoforms of Hexokinase (1~4) in recombinant protein. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | HK1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 10% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: Hexokinase 1 Antibody (4D7) [NBP1-04295] - Human HeLa cells stained with Hoechst 33342 (Blue) and monoclonal antiHexokinase antibody (1:1000) with Alexa 488 (Green).](http://images.novusbio.com/fullsize/Hexokinase-1-Antibody-4D7-Immunocytochemistry-Immunofluorescence-NBP1-04295-img0008.jpg "Immunocytochemistry/Immunofluorescence: Hexokinase 1 Antibody (4D7) [NBP1-04295] - Human HeLa cells stained with Hoechst 33342 (Blue) and monoclonal antiHexokinase antibody (1:1000) with Alexa 488 (Green).")
![Western Blot: Hexokinase 1 Antibody (4D7) [NBP1-04295] - The tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Brain tissue lysate](http://images.novusbio.com/fullsize/Hexokinase-1-Antibody-4D7-Western-Blot-NBP1-04295-img0009.jpg "Western Blot: Hexokinase 1 Antibody (4D7) [NBP1-04295] - The tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Brain tissue lysate")
![Western Blot: Hexokinase 1 Antibody (4D7) [NBP1-04295] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: HeLa cell lysate Lane 3.: Jurkat cell lysate Lane 4.: K562 cell lysate](http://images.novusbio.com/fullsize/Hexokinase-1-Antibody-4D7-Western-Blot-NBP1-04295-img0010.jpg "Western Blot: Hexokinase 1 Antibody (4D7) [NBP1-04295] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: HeLa cell lysate Lane 3.: Jurkat cell lysate Lane 4.: K562 cell lysate")
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