Recombinant Human HES2 His Protein Summary
Description |
A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-173 of Human HES2 Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGLPRRA GDAAELRKSL KPLLEKRRRA RINQSLSQLK GLILPLLGRE NSNCSKLEKA DVLEMTVRFL QELPASSWPT AAPLPCDSYR EGYSACVARL ARVLPACRVL EPAVSARLLE HLWRRAASAT LDGGRAGDSS GPSAPAPAPA SAPEPASAPV PSPPSPPCGP GLWRPW |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
HES2 |
Purity |
>80%, by SDS-PAGE |
Applications/Dilutions
Dilutions |
|
Application Notes |
Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays. |
Theoretical MW |
20.9 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol |
Preservative |
No Preservative |
Concentration |
1 mg/ml |
Purity |
>80%, by SDS-PAGE |
Alternate Names for Recombinant Human HES2 His Protein
Background
Hairy and enhancer of split 2, also known as HES2, is a member of the HES family and contains one basic helix-loop-helix (bHLH) domain and one orange domain. The HES family members form a complex with TLE, the mammalian homologue of Groucho, and this interaction is mediated by the carboxy terminal WRPW motif of the HES proteins. Activation of Notch signaling pathway leads to activation of HES family genes through the interaction between Notch intracellular domain and RBPSUH (CSL). HES2 is expressed in a variety of adult and embryonic tissue. Recombinant human HES2 protein, fused to His-tag at N-terminus, was expressed in E.coli.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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