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HES-4 Antibody [Unconjugated]

Name: HES-4 Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF3600
Price: 459 USD

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HES-4 was detected in immersion fixed HEK293 human embryonic kidney cell line using Sheep Anti-Human HES-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3600) at 1.7 µg/mL for 3 hours at room temperature. ...read more

Product Details

Summary

Reactivity	HuSpecies Glossary
Applications	WB, ICC/IF
Clonality	Polyclonal
Host	Sheep
Conjugate	Unconjugated
Concentration	LYOPH

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Catalog# & Conjugate	Size	Price
Biotin BAF3600

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Catalog# & Formulation	Size	Price
Carrier Free AF3600-SP

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Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF3600

HES-4 Antibody [Unconjugated] Summary

Immunogen	E. coli-derived recombinant human HES-4 Val94-Arg221 Accession # Q9HCC6
Specificity	Detects human HES-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) HES-1, rhHES-2, rhHES-5, and rhHES-7 is observed.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Sheep
Gene	HES4
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions

Immunocytochemistry 5-15 ug/mL
Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HES-4 Antibody [Unconjugated]

bHLH factor Hes4
BHLHB42
bHLHb42transcription factor HES-4
Class B basic helix-loop-helix protein 42
hairy and enhancer of split 4 (Drosophila)
Hairy and enhancer of split 4
HES4
HES-4
hHES4

Background

HES-4 is a 221 aa, 24 kDa member of the HES-family of transcriptional repressors. HES-4 contains a species-specific bHLH domain (aa 33‑93), a specific orange DNA-binding domain (aa 109‑150), a 65 aa Proline-rich SH3-binding domain, and a WRPW Groucho/TLE interacting C-terminal motif. HES-4 is a HES-1 paralog likely to maintain stem cell pluripotency. In the region used as immunogen, the amino acid sequence of human and canine HES-4 are 80% identical.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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HES-4 Antibody
[Unconjugated]

AF3600

Species: Hu

Applications: WB, ICC/IF

Publications for HES-4 Antibody (AF3600) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

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Sheep IgG Isotype Control [Unconjugated]
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Bioinformatics

Gene Symbol	HES4
Uniprot	Q9HCC6()

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Antibodies Secondary Antibodies Isotype Controls Proteins and Peptides ELISA Kits Sample Size New Products	About Us Careers Novus Guarantee Contact Us Our Team	Technical Support FAQs Help Finding Antibodies Protocols

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

HES-4 Antibody [Unconjugated]

Species Glossary

Images

Product Details

Order Details

HES-4 Antibody [Unconjugated] Summary

Applications/Dilutions

Packaging, Storage & Formulations

Notes

Alternate Names for HES-4 Antibody [Unconjugated]

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for HES-4 Antibody (AF3600) (0)

Reviews for HES-4 Antibody (AF3600) (0)

Product General Protocols

Video Protocols

FAQs for HES-4 Antibody (AF3600) (0)

Secondary Antibodies

Isotype Controls

Additional HES-4 Products

Blogs on HES-4

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Review this Product

Bioinformatics