Western Blot: HES5 Antibody [NBP2-41305] - Western blot analysis of HES5 in Raji cell lysate with HES5 antibody at 1 ug/ml (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: HES5 Antibody [NBP2-41305] - Staining of HES5 in mouse brain tissue with at 20 ug/mL.
Immunohistochemistry-Paraffin: HES5 Antibody [NBP2-41305] - Staining of HES5 in mouse brain tissue with at 5 ug/mL.
Immunocytochemistry/ Immunofluorescence: HES5 Antibody [NBP2-41305] - Human spleen tissue with HES5 antibody at 20 ug/ml.
Immunohistochemistry-Paraffin: HES5 Antibody [NBP2-41305] - Human spleen tissue with HES5 antibody at 5 ug/ml.
Antibody was raised against a 19 amino acid peptide near the amino terminus of human HES5. The immunogen is located within the first 50 amino acids of HES5. Amino Acid Squence: EKNRLRKPVVEKMRRDRIN
Specificity
At least two isoforms of HES5 are known to exist; this antibody will detect both isoforms.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HES5
Purity
Peptide affinity purified
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17 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified
Alternate Names for HES5 Antibody - BSA Free
BHLHB38
bHLHb38Class B basic helix-loop-helix protein 38
hairy and enhancer of split 5 (Drosophila)
Hairy and enhancer of split 5
transcription factor HES-5
Background
GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Recombinant GFP protein was expressed in E.coli and purified by using conventional chromatography.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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