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RRBP1 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957] - Analysis in human placenta and skeletal muscle tissues. Corresponding RRBP1 RNA-seq data are presented for the same tissues.
Independent Antibodies: Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957] - Staining of human colon, kidney, lymph node and placenta using Anti-RRBP1 antibody NBP1-83957 (A) shows similar protein ...read more
Western Blot: RRBP1 Antibody [NBP1-83957] - Analysis in human cell line CACO-2.
Immunocytochemistry/ Immunofluorescence: RRBP1 Antibody [NBP1-83957] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957] - Staining of human placenta using Anti-RRBP1 antibody NBP1-83957.
Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957] - Staining of human skeletal muscle shows weak cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957] - Staining of human kidney.
Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957] - Staining of human colon.
Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-83957] - Staining of human lymph node.
Simple Western: RRBP1 Antibody [NBP1-83957] - Simple Western lane view shows a specific band for RRBP1 in 0.2 mg/ml of h. Colon lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Simple Western: RRBP1 Antibody [NBP1-83957] - Electropherogram image of the corresponding Simple Western lane view. RRBP1 antibody was used at 1:200 dilution on h. Colon lysate(s) respectively.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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RRBP1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ELHSKLQSSEAEVRSKCEELSGLHGQLQEARAENSQLTERIRSIEALLEAGQARDAQDVQASQAEADQQQTRLKELESQVSGLEKEAIELREAVEQQKVKNNDLREK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RRBP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Simple Western 1:200
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Control Peptide
RRBP1 Protein (NBP1-83957PEP)
Publications
Read Publication using NBP1-83957.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (86%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RRBP1 Antibody

  • 180 kDa ribosome receptor homolog
  • DKFZp586A1420
  • ES/130
  • ES/130-related protein
  • ES130
  • FLJ36146
  • hES
  • KIAA1398
  • MGC157720
  • MGC157721
  • ribosome binding protein 1 (dog 180kD homolog)
  • ribosome binding protein 1 homolog 180kDa (dog)
  • Ribosome receptor protein
  • ribosome-binding protein 1
  • RRp

Background

Analysis of cDNA clones indicates that ribosome binding protein 1 may exist in different forms due to removal of tandem repeats, or partial intraexonic splicing of RRBP1. The form presented here is lacking the canine p180 ribosome-binding domain, NQGKKAEGAQ, which is tandemly repeated close to the N-terminus in other forms that haven't been fully characterized. RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome. Alternate splicing results in multiple transcript variants. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RRBP1 Antibody (NBP1-83957)(1)

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol RRBP1