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HADH Antibody (OTI3D12)

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Western Blot: HADH Antibody (3D12) [NBP2-45931] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HADH.
Western Blot: HADH Antibody (3D12) [NBP2-45931] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HADH.
Immunohistochemistry: HADH Antibody (3D12) [NBP2-45931] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: HADH Antibody (3D12) [NBP2-45931] - Analysis of HT29 cell lysate (35ug) by using HADH monoclonal antibody.
Immunohistochemistry: HADH Antibody (3D12) [NBP2-45931] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: HADH Antibody (3D12) [NBP2-45931] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: HADH Antibody (3D12) [NBP2-45931] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: HADH Antibody (3D12) [NBP2-45931] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI3D12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70875AF350
Alexa Fluor 405
NBP2-70875AF405
Alexa Fluor 488
NBP2-70875AF488
Alexa Fluor 532
NBP2-70875AF532
Alexa Fluor 594
NBP2-70875AF594
Alexa Fluor 647
NBP2-70875AF647
Alexa Fluor 700
NBP2-70875AF700
Alexa Fluor 750
NBP2-70875AF750
Allophycocyanin
NBP2-70875APC
Biotin
NBP2-70875B
CoraFluor 1
NBP2-70875CL1
DyLight 350
NBP2-70875UV
DyLight 405
NBP2-70875V
DyLight 488
NBP2-70875G
DyLight 550
NBP2-70875R
DyLight 594
NBP2-70875DL594
DyLight 650
NBP2-70875C
DyLight 680
NBP2-70875FR
DyLight 755
NBP2-70875IR
FITC
NBP2-70875F
HRP
NBP2-70875H
Janelia Fluor 525
NBP2-70875JF525
Janelia Fluor 549
NBP2-70875JF549
Janelia Fluor 646
NBP2-70875JF646
Janelia Fluor 669
NBP2-70875JF669
PE
NBP2-70875PE
PerCP
NBP2-70875PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-70875
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HADH Antibody (OTI3D12) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 57-314 of human HADH (NP_005318) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HADH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
32.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HADH Antibody (OTI3D12)

  • EC 1.1.1
  • EC 1.1.1.35
  • HADH1
  • HADHSChydroxyacyl-Coenzyme A dehydrogenase
  • HCDH
  • hydroxyacyl-CoA dehydrogenase
  • L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain
  • Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase
  • MGC8392
  • mitochondrial
  • MSCHAD
  • SCHADHHF4

Background

HADH is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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HADH Antibody (OTI3D12)
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Bioinformatics

Gene Symbol HADH