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GTF2H1 Antibody

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Western Blot: GTF2H1 Antibody [NBP2-16761] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with GTF2H1 antibody [N1C1] diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: GTF2H1 Antibody [NBP2-16761] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GTF2H1 stained by GTF2H1 antibody [N1C1] diluted at 1:500. Red: phalloidin, ...read more
Western Blot: GTF2H1 Antibody [NBP2-16761] - Sample (30 ug of whole cell lysate) A: HepG2 7. 5% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ChSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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GTF2H1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human GTF2H1. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Mouse (98%), Rat (98%), Chicken (92%), Bovine (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GTF2H1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (83%), Xenopus laevis (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for GTF2H1 Antibody

  • Basic transcription factor 2 62 kDa subunit
  • BTF2 p62
  • BTF2general transcription factor IIH, polypeptide 1 (62kD subunit)
  • General transcription factor IIH polypeptide 1
  • general transcription factor IIH subunit 1
  • general transcription factor IIH, polypeptide 1, 62kDa
  • TFB1
  • TFIIH basal transcription factor complex p62 subunit
  • TFIIH

Background

GTF2H1 is a component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNAand, when complexed to CAK, in RNA transcription by RNA polymerase II

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GTF2H1
Uniprot