Human GRIP1 ELISA Kit (Colorimetric) Summary
Description |
Assay Length: 4.5 hours |
Standard Curve Range |
0.312 - 20 ng/mL (example only; lot dependent) |
Sensitivity |
0.118 ng/mL (example only; lot dependent) |
Assay Type |
Sandwich ELISA |
Inter-Assay |
%CV < 12 (example only; lot dependent) |
Intra-Assay |
%CV < 10 (example only; lot dependent) |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
GRIP1 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Detection Reagent A
- Detection Reagent B
- Diluent Buffer
- Instruction manual
- Plate sealer for 96 wells
- Pre-coated 96T strip plate
- Standard
- Stop Solution
- TMB Substrate
- Wash Buffer (30 x concentrate)
|
Alternate Names for Human GRIP1 ELISA Kit (Colorimetric)
Background
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Glucocorticoid receptor interacting protein-1 (GRIP 1), or Transcription Intermediary Factor 2 (TIF 2), is a member of a family of transcriptional coactivator proteins which includes steroid receptor coactivator-1 (SRC 1) and Amplified in breast cancer-1 (AIB 1).GRIP1 has been shown to interact and stimulate transcriptional activity of the retinoic acid, retinoid X, vitamin D, mineralocorticoid, glucocorticoid, thyroid hormone, androgen, estrogen, progesterone, and peroxisome proliferator activated receptors. GRIP 1 also contains several copies of the conserved LXXLL/LLXXL motif which has been demonstrated to be critical to receptor-coactivator interactions.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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