![Western Blot: GPR125 Antibody [NBP3-35575] - Western blot analysis of lysates from Mouse liver, using GPR125 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3min.](http://images.novusbio.com/fullsize/nbp3-35575_rabbit-gpr125-pab-101220241214491.jpg "Western Blot: GPR125 Antibody [NBP3-35575] - Western blot analysis of lysates from Mouse liver, using GPR125 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3min.")
| Reactivity | Mu, RtSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 370-600 of human GPR125 (NP_660333.2). Sequence: ITAYLQCTRNTHGSGIYPGNPQDERKAWRRCDRGGFWADDDYSRCQYANDVTRVLYMFNQMPLNLTNAVATARQLLAYTVEAANFSDKMDVIFVAEMIEKFGRFTKEEKSKELGDVMVDIASNIMLADERVLWLAQREAKACSRIVQCLQRIATYRLAGGAHVYSTYSPNIALEAYVIKSTGFTGMTCTVFQKVAASDRTGLSDYGRRDPEGNLDKQLSFKCNVSNTFSSL |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ADGRA3 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 146 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Affinity purified |
![Flow Cytometry TEM5/GPR124 Antibody (499716) [Unconjugated]](https://images.novusbio.com/images2/TEM5_MAB8896_Flow_Cytometry_17891.jpg)
![Simple Western CD117/c-kit Antibody [Unconjugated]](https://images.novusbio.com/images2/CD117_AF1356_Simple_Western_17480.jpg)
![Western Blot CD117/c-kit Antibody [Unconjugated]](https://images.novusbio.com/images2/CD117_AF1356_Western_Blot_18637.jpg)
![Immunohistochemistry CD117/c-kit Antibody [Unconjugated]](https://images.novusbio.com/images2/SCF_R_AF1356_Immunohistochemistry_9746.jpg)
![Flow Cytometry EMR3 Antibody (908235) [Unconjugated]](https://images.novusbio.com/images2/EMR3_MAB8496_Flow_Cytometry_16807.jpg)
![Immunohistochemistry EMR3 Antibody (908235) [Unconjugated]](https://images.novusbio.com/images2/mab8496_human-emr3-mab-clone-908235-immunohistochemistry-2852021145344.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for GPR125 Antibody (NBP3-35575)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ADGRA3 |
![Immunohistochemistry GFR alpha-1/GDNF R alpha-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/GFR_alpha1_AF560_Immunohistochemistry_17364.jpg)
![Western Blot GFR alpha-1/GDNF R alpha-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/GFR_alpha-1_AF560_Western_Blot_12576.jpg)
![Immunocytochemistry/ Immunofluorescence GFR alpha-1/GDNF R alpha-1 Antibody [Unconjugated]](https://images.novusbio.com/images/af560_rat-gfr-alpha-1-gdnf-r-alpha-1-affinity-purified-pab-8220249505912.jpg)
![Flow Cytometry GPR64 Antibody [Unconjugated]](https://images.novusbio.com/images2/GPR64_AF7977_Flow_Cytometry_22399.jpg)
![Western Blot GPR64 Antibody [Unconjugated]](https://images.novusbio.com/images2/GPR64_AF7977_Western_Blot_13069.jpg)
![Immunohistochemistry GPR64 Antibody [Unconjugated]](https://images.novusbio.com/images2/GPR64_AF7977_Immunohistochemistry_13077.jpg)
![Chromatin Immunoprecipitation (ChIP) Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct-3_4_AF1759_Chromatin_Immunoprecipitation_11218.jpg)
![Immunocytochemistry Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct-3_4_AF1759_Immunocytochemistry_7093.jpg)
![Simple Western Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct3_4_AF1759_Simple_Western_20183.jpg)
![Western Blot GPR56 Antibody [Unconjugated]](https://images.novusbio.com/images2/GPR56_AF4634_Western_Blot_11569.jpg)
![Simple Western GPR56 Antibody [Unconjugated]](https://images.novusbio.com/images2/GPR56_AF4634_Simple_Western_22172.jpg)
![Flow Cytometry GPR56 Antibody [Unconjugated]](https://images.novusbio.com/images2/GPR56_AF4634_Flow_Cytometry_12104.jpg)
![Western Blot ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg)
![Immunohistochemistry ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg)
![Knockout Validated ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")