![Western Blot: Glyoxalase I Antibody [NBP2-98271] - Anti-Glyoxalase I rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 23 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-Western-Blot-NBP2-98271-img0004.jpg "Western Blot: Glyoxalase I Antibody [NBP2-98271] - Anti-Glyoxalase I rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 23 kDa (We are unsure as to the identity of these extra bands).")
| Reactivity | MuSpecies Glossary |
| Applications | WB, ELISA, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Immunogen | Produced in rabbits immunized with purified, recombinant Mouse Glyoxalase I (Accession#: NP_079650.3; Ala 2-Ile 184) |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GLO1 |
| Purity | Antigen and protein A Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Antigen and protein A Affinity-purified |
![Western Blot Glyoxalase II/HAGH Antibody [Unconjugated]](https://images.novusbio.com/images2/Glyoxalase_II_AF5944_Western_Blot_5888.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Glyoxalase I Antibody (NBP2-98271)Find related products by research area.
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| Gene Symbol | GLO1 |
![Immunoprecipitation: Glyoxalase I Antibody [NBP2-98271] - Mouse Glyoxalase I was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. Lane B: 0.5 mg A549 Whole Cell Lysate. Lane C: 0.5 mg HepG2 Whole Cell Lysate. 2 ul anti-Mouse Glyoxalase I rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse Glyoxalase I rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 24 kDa](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-Immunoprecipitation-NBP2-98271-img0003.jpg "Immunoprecipitation: Glyoxalase I Antibody [NBP2-98271] - Mouse Glyoxalase I was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. Lane B: 0.5 mg A549 Whole Cell Lysate. Lane C: 0.5 mg HepG2 Whole Cell Lysate. 2 ul anti-Mouse Glyoxalase I rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse Glyoxalase I rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 24 kDa")
![N/A Haptoglobin [HRP]](https://images.novusbio.com/images2/Haptoglobin_DHAPG0_ELISA_461.jpg)
![N/A Haptoglobin [HRP]](https://images.novusbio.com/images2/DATA_Haptoglobin_DHAPG0_ELISA_709.jpg)
![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
![Simple Western Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Simple_Western_16990.jpg)
![Knockout Validated Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Knockout_Validated_24032.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")