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Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free

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Western Blot: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutathione Synthetase (Right lane) cDNA ...read more
Immunohistochemistry: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - Staining of paraffin-embedded Human Kidney tissue using anti-Glutathione Synthetase mouse monoclonal antibody.
Flow Cytometry: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining ...read more
Immunohistochemistry: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Glutathione Synthetase mouse monoclonal ...read more
Immunohistochemistry: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - Staining of paraffin-embedded Human prostate tissue using anti-Glutathione Synthetase mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clone
OTI1B8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human GSS (NP_000169) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GSS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
52.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free

  • EC 6.3.2.3
  • Glutathione synthase
  • glutathione synthetase
  • GSH synthetase
  • GSHS
  • GSH-S
  • MGC14098

Background

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage byfree radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as ahomodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion ofgamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.(provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GSS