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Glutathione S Transferase kappa 1 Antibody

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Genetic Strategies: Western Blot: Glutathione S Transferase kappa 1 Antibody [NBP2-16685] - Wild-type (WT) and Glutathione S Transferase kappa 1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% ...read more
Immunocytochemistry/ Immunofluorescence: Glutathione S Transferase kappa 1 Antibody [NBP2-16685] - Sample: HeLa cells were fixed in iced-cold MeOH for 5 min. Green: GSTK1 protein stained by Glutathione S-transferase ...read more
Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody [NBP2-16685] - Analysis of Cal27 xenograft, using antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min.
Western Blot: Glutathione S Transferase kappa 1 Antibody [NBP2-16685] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 12% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, RMSpecies Glossary
Applications WB, ICC/IF, IHC, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

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Glutathione S Transferase kappa 1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Glutathione S Transferase kappa 1. The exact sequence is proprietary.
Localization
Peroxisome
Predicted Species
Rhesus Macaque (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GSTK1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Pig (83%), Bovine (80%)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Glutathione S Transferase kappa 1 Antibody

  • EC 2.5.1.18
  • glutathione S-transferase k1
  • glutathione S-transferase kappa 1
  • glutathione S-transferase subunit 13 homolog
  • Glutathione S-transferase subunit 13
  • GST 13-13
  • GST class-kappa
  • GST
  • GST13
  • GST13-13
  • GSTK1-1
  • hGSTK1

Background

This gene encodes a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification. The encoded protein is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. Alternative splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol GSTK1
Uniprot