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Glutathione Peroxidase 2/GPX2 Antibody

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Genetic Strategies: Western Blot: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane ...read more
Western Blot: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - Glutathione Peroxidase 2/GPX2 antibody detects Glutathione Peroxidase 2/GPX2 protein by Western blot analysis. Whole cell extracts (30 ug) was ...read more
Western Blot: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - Glutathione Peroxidase 2/GPX2 antibody detects GPX2 protein by Western blot analysis. Mouse tissue extracts (50 ug) was separated by 12 % SDS-PAGE, and ...read more
Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - Human gastric cancer, using antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunoprecipitation: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - Immunoprecipitation of GPX2 protein from HepG2 whole cell extracts using 5 ug of GPX2 antibody [C1C3] . Western blot analysis was performed ...read more
Western Blot: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [C1C3] diluted at 1:1000. The ...read more
Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - Mouse stomach.GPX2 stained by GPX2 antibody [C1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] -Rat duodenum.GPX2 stained by GPX2 antibody [C1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - Mouse stomach.GPX2 stained by GPX2 antibody [C1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ICC/IF-Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GPX2 stained by GPX2 antibody [C1C3] diluted at 1:500.Red: alpha Tubulin, a cytoskeleton ...read more
Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - Mouse intestine.GPX2 stained by GPX2 antibody [C1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - Rat liver.GPX2 stained by GPX2 antibody [C1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - Glutathione Peroxidase 2/GPX2 antibody [C1C3] detects Glutathione Peroxidase 2/GPX2 protein at cytoplasm by immunohistochemical ...read more
Immunohistochemistry: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - Immunohistochemical analysis of paraffin-embedded mouse muscle, using Glutathione Peroxidase 2/GPX2(NBP1-32002) antibody at 1:500 ...read more
Orthogonal Strategies: Western Blot: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody ...read more
Genetic Strategies: Western Blot: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Pm, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Glutathione Peroxidase 2/GPX2 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Glutathione Peroxidase 2/GPX2. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Porcine (100%), Chimpanzee (100%), Bovine (100%), Rhesus Macaque (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GPX2
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:10000
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for Glutathione Peroxidase 2/GPX2 Antibody

  • EC 1.11.1
  • EC 1.11.1.9
  • gastrointestinal glutathione peroxidase 2
  • Gastrointestinal glutathione peroxidase
  • GIGPX
  • GI-GPx
  • glutathione peroxidase 2 (gastrointestinal)
  • Glutathione Peroxidase 2
  • Glutathione peroxidase-gastrointestinal
  • Glutathione peroxidase-related protein 2
  • GPRP
  • GPRP-2
  • GPX2
  • GPx-2
  • GPx-GI
  • GSHPx-2
  • GSHPX-GI

Background

This gene is a member of the glutathione peroxidase family and encodes a selenium-dependent glutathione peroxidase that is one of two isoenzymes responsible for the majority of the glutathione-dependent hydrogen peroxide-reducing activity in the epithelium of the gastrointestinal tract. Studies in knockout mice indicate that mRNA expression levels respond to luminal microflora, suggesting a role of the ileal glutathione peroxidases in preventing inflammation in the GI tract.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol GPX2