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GIT1 Antibody (S39B-8) [Alexa Fluor® 647]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC, IP, KO
Clone
S39B-8
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 647

GIT1 Antibody (S39B-8) [Alexa Fluor® 647] Summary

Immunogen
Fusion protein amino acids 375-770 (C-terminus) of rat GIT1
Localization
Cytoplasm
Specificity
Detects approx 90kDa. Does not cross-react with GIT2.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GIT1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Knockout Validated
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30546041).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for GIT1 Antibody (S39B-8) [Alexa Fluor® 647]

  • ARF GAP GIT1
  • ARF GTPase-activating protein GIT1
  • CAT1
  • CAT-1
  • Cool-associated and tyrosine-phosphorylated protein 1
  • G protein-coupled receptor kinase interacting ArfGAP 1
  • G protein-coupled receptor kinase interactor 1
  • G protein-coupled receptor kinase-interactor 1
  • GIT1
  • GRK-interacting protein 1

Background

G-protein-coupled receptor kinase interactor 1 (GIT1) and G-protein-coupled receptor kinase interactor 2 (GIT2) are GTPase-activating proteins for ADP-ribosylation (Arf) GTPases. GIT1 and GIT2 regulate Arf activity and the sequestration of activated G protein-coupled receptors from the surface of the cell. These activities have effects on multiple cellular processes such as cell migration, T-cell activation, and centrosome dynamics. Both GIT proteins also serve as scaffolds to link signaling molecules. Alternative names for GIT1 include GRK-interacting protein 1, cool-associated and tyrosine phosphorylated protein 1, and Cat-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for GIT1 Antibody (NBP2-22423AF647) (0)

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Secondary Antibodies

 

Isotype Controls

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Contact Information

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Bioinformatics

Gene Symbol GIT1