GFAP Antibody (5C10)

Glial fibrillary acidic protein (GFAP) is a class III intermediate filament protein that is largely expressed in astrocytes in addition to non-myelinating Schwann cells and glial cells (1,2). Other members of the type III intermediate filament family include desmin, peripherin, and vimentin (2-4). GFAP was first identified in the brains of multiple sclerosis patients (2). Human GFAP protein is 432 amino acids in length with a theoretical molecular weight of ~50 kDa (1,3). GFAP has at least 10 known isoforms, with the most prevalent and common in the brain being GFAP-alpha which is made of a head domain, a rod domain with four coils (1A, 1B, 2A, 2B) joined by linker regions, and a tail domain (1). GFAP is a marker of mature astrocytes, but is also expressed throughout development in both fetal and adult neural stem cells (2). While the exact function of GFAP is still elusive, it has been shown to play a role in cellular processes such as migration, mitosis, structural integrity, and signaling (2).

An increase in GFAP levels is often associated with neuroinflammation which results in the activation and proliferation of astroglia cell population (1,2). GFAP expression is also observed in brains of patients with neurodegenerative diseases including Alzheimer's and Parkinson's, epilepsy disorders, and brain injuries (1-4). Lesion sites associated with neurodegeneration can exhibit an array of gliosis characteristics from glial scarring with reduced astrocyte proliferation to activated, GFAP-positive astrocytes surrounding amyloid plaques (2). Furthermore, the GFAP gene is a target of single nucleotide polymorphisms in the coding region, considered a gain-of-function mutation, characterized by astrocytic inclusions, termed Rosenthal fibers, resulting in Alexander Disease (1-4). GFAP is also a center of many post-translational modifications, such as phosphorylation, which can alter various aspects of filament assembly (1,4).

References

1. Yang, Z., & Wang, K. K. (2015). Glial fibrillary acidic protein: from intermediate filament assembly and gliosis to neurobiomarker. Trends in Neurosciences. https://doi.org/10.1016/j.tins.2015.04.003

2. Hol, E. M., & Capetanaki, Y. (2017). Type III Intermediate Filaments Desmin, Glial Fibrillary Acidic Protein (GFAP), Vimentin, and Peripherin. Cold Spring Harbor Perspectives in Biology. https://doi.org/10.1101/cshperspect.a021642

3. Potokar, M., Morita, M., Wiche, G., & Jorgacevski, J. (2020). The Diversity of Intermediate Filaments in Astrocytes. Cells. https://doi.org/10.3390/cells9071604

4. Viedma-Poyatos, a., Pajares, M. A., & Perez-Sala, D. (2020). Type III intermediate filaments as targets and effectors of electrophiles and oxidants. Redox Biology. https://doi.org/10.1016/j.redox.2020.101582

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 10 applications: Block/Neutralize, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, IHC/IF, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, WB.

Showing Publications 1 - 10 of 27. Show All 27 Publications. Collapse Publications.

Publications using NBP1-05197	Applications	Species
Laura Jahnke, Virginie Perrenoud, Souska Zandi, Yuebing Li, Federica Maria Conedera, Volker Enzmann, Girish Kumar Srivastava Modulation of Extracellular Matrix Composition and Chronic Inflammation with Pirfenidone Promotes Scar Reduction in Retinal Wound Repair Cells 2024-01-16 [PMID: 38247855]
Duan Q, Zhang Q, Nie K et al. Myo1d Promotes Alpha-Synuclein Transfer from Brain Microvascular Endothelial Cells to Pericytes through Tunneling Nanotubes iScience 2023-07-01 [PMID: 37575183] (ICC/IF, Mouse) Details: Dilutions: 1:100	ICC/IF	Mouse
Rueda-Gensini L, Serna JA, Rubio D et al. Three-dimensional neuroimmune co-culture system for modeling Parkinson's Disease microenvironments in vitro Biofabrication 2023-06-27 [PMID: 37369196] (Immunocytochemistry/ Immunofluorescence, Human)	Immunocytochemistry/ Immunofluorescence	Human
Jahnke L, Zandi S, Elhelbawi A et al. Characterization of Macroglia Response during Tissue Repair in a Laser-Induced Model of Retinal Degeneration International Journal of Molecular Sciences 2023-05-24 [PMID: 37298126] (IHC-P, Mouse) Details: 1:200 IHC-P dilution	IHC-P	Mouse
Tseng KY, Stratoulias V, Hu WF et al. Augmenting hematoma-scavenging capacity of innate immune cells by CDNF reduces brain injury and promotes functional recovery after intracerebral hemorrhage Cell death & disease 2023-02-15 [PMID: 36792604] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Wong JK, Lin J, Kung NJ et al. Cerebrospinal fluid immunoglobulins in primary progressive multiple sclerosis are pathogenic Brain : a journal of neurology 2023-02-03 [PMID: 36732292] (IHC, Mouse)	IHC	Mouse
Griflyuk AV, Postnikova TY, Zaitsev AV. Prolonged Febrile Seizures Impair Synaptic Plasticity and Alter Developmental Pattern of Glial Fibrillary Acidic Protein (GFAP)-Immunoreactive Astrocytes in the Hippocampus of Young Rats International Journal of Molecular Sciences 2022-10-13 [PMID: 36293077] (Block/Neutralize, Immunocytochemistry/ Immunofluorescence)	Block/Neutralize, Immunocytochemistry/ Immunofluorescence
Becker-Krail DD, Ketchesin KD, Burns JN et al. Astrocyte Molecular Clock Function in the Nucleus Accumbens Is Important for Reward-Related Behavior Biological Psychiatry 2022-07-01 [PMID: 35461698] (Block/Neutralize)	Block/Neutralize
Postnikova TY, Diespirov GP, Amakhin DV et al. Impairments of Long-Term Synaptic Plasticity in the Hippocampus of Young Rats during the Latent Phase of the Lithium-Pilocarpine Model of Temporal Lobe Epilepsy International Journal of Molecular Sciences 2021-12-12 [PMID: 34948152] (Immunohistochemistry, Block/Neutralize, Immunocytochemistry/ Immunofluorescence)	Immunohistochemistry, Block/Neutralize, Immunocytochemistry/ Immunofluorescence
Fayaz MA, Rosa GDS, Honaramooz A. Neonatal Porcine Germ Cells Dedifferentiate and Display Osteogenic and Pluripotency Properties Cells 2021-10-20 [PMID: 34831039] (ICC/IF)	ICC/IF
Scaricamazza S, Salvatori I, Amadio S et al. Repurposing of Trimetazidine for amyotrophic lateral sclerosis: A study in SOD1(G93A) mice British Journal of Pharmacology 2022-04-01 [PMID: 34783031]
Zhan H, Wang Y, Yu S et al. Upregulation of Mlxipl induced by cJun in the spinal dorsal horn after peripheral nerve injury counteracts mechanical allodynia by inhibiting neuroinflammation Aging (Albany NY) 2020-06-09 [PMID: 32518215] (ICC/IF, Rat)	ICC/IF	Rat
Alshammari TK, Alghamdi H, Green TA et al. Assessing the role of toll-like receptor in isolated, standard and enriched housing conditions PLoS ONE 2019-10-24 [PMID: 31647818] (ICC/IF, Mouse)	ICC/IF	Mouse
Yazdankhah M, Shang P, Ghosh S et al. Modulating EGFR-MTORC1-autophagy as a potential therapy for persistent fetal vasculature (PFV) disease Autophagy 2019-09-01 [PMID: 31462148] (IHC/IF, Rat)	IHC/IF	Rat
Sivaguru M, Khaw YM, Inoue M A Confocal Reflection Super-Resolution Technique to Image Golgi-Cox Stained Neurons J Microsc 2019-06-25 [PMID: 31237354]
Murakami T, Miyoshi T, Takahashi N, Kangawa A. Persistent hyperplastic primary vitreous in two piglets. J. Vet. Med. Sci. 2019-01-15 [PMID: 30643102] (IF/IHC, Porcine)	IF/IHC	Porcine
Lananna B V, Nadarajah C J et al. Cell-Autonomous Regulation of Astrocyte Activation by the Circadian Clock Protein BMAL1. Cell Rep 2018-02-10 [PMID: 30282019] (IF/IHC, Mouse)	IF/IHC	Mouse
Hwang S, Ham S, Lee SE et al. Hypoxia regulates the level of glutamic acid decarboxylase enzymes and interrupts inhibitory synapse stability in primary cultured neurons Neurotoxicology 2017-10-28 [PMID: 29107683] (WB, Mouse)	WB	Mouse
Filice F, Celio MR, Babalian A et al. Parvalbumin-expressing ependymal cells in rostral lateral ventricle wall adhesions contribute to aging-related ventricle stenosis in mice J. Comp. Neurol. 2017-07-04 [PMID: 28675430] (IF/IHC, Mouse)	IF/IHC	Mouse
Jurga AM, Piotrowska A, Makuch W et al. Blockade of P2X4 Receptors Inhibits Neuropathic Pain-Related Behavior by Preventing MMP-9 Activation and, Consequently, Pronociceptive Interleukin Release in a Rat Model. Front Pharmacol. 2017-03-09 [PMID: 28275350] (WB, Rat)	WB	Rat
Pacheco-Quinto J, Eckman CB, Eckman EA. Major amyloid-beta-degrading enzymes, endothelin-converting enzyme-2 and neprilysin, are expressed by distinct populations of GABAergic interneurons in hippocampus and neocortex. Neurobiol Aging 2016-08-20 [PMID: 27644077]
Bang Y, Kim KS, Seol W, Choi HJ. LRRK2 interferes with aggresome formation for autophagic clearance. Mol Cell Neurosci 2016-09-01 [PMID: 27364102] (ICC/IF, Mouse)	ICC/IF	Mouse
Nemery E, Gabriel A, Piret J, Antoine N. Nociceptive and sympathetic innervations in the abaxial part of the cranial horn of the equine medial meniscus: an immunohistochemical approach. J Anat 2016-12-01 [PMID: 27345299]
Gebremedhin D, Zhang DX, Carver KA et al. Expression of CYP 4A omega-hydroxylase and formation of 20-hydroxyeicosatetreanoic acid (20-HETE) in cultured rat brain astrocytes. Prostaglandins Other Lipid Mediat. 2016-05-09 [PMID: 27174801] (ICC/IF, Rat)	ICC/IF	Rat
Szabolcsi V, Celio Mr. De novo expression of parvalbumin in ependymal cells in response to brain injury promotes ependymal remodeling and wound repair. Glia. 2014-11-24 [PMID: 25421913] (IF/IHC, Mouse)	IF/IHC	Mouse
Brai Emanuele, Marathe Swananda, Zentilin Lorena et al. Notch1 activity in the olfactory bulb is odour-dependent and contributes to olfactory behaviour. Eur J Neurosci. 2014-09-19 [PMID: 25234246]
Hernandez, C M, Cortez, I Et al. Research tool: Validation of floxed alpha 7 nicotinic acetylcholine receptor conditional knockout mice using in vitro and in vivo approaches. J Physiol 2014-08-01 [PMID: 24879866] (IF/IHC, Mouse)	IF/IHC	Mouse
Show All 27 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	3 reviewed by: Verified Customer	IF	Mouse	05/11/2016	View Summary Application Immunofluorescence Sample Tested mouse spinal cord Species Mouse Lot 5313

Array NBP1-05197

• GFAP Antibodies
• GFAP ELISA Kits
• GFAP Lysate
• GFAP Proteins
• GFAP ELISA