![Western Blot: GALNT2 Antibody [NBP3-00188] - Anti-GALNT2 rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lane C: U87MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 65 kDa. Observed band size: 65 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/GALNT2-Antibody-Western-Blot-NBP3-00188-img0006.jpg "Western Blot: GALNT2 Antibody [NBP3-00188] - Anti-GALNT2 rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lane C: U87MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 65 kDa. Observed band size: 65 kDa (We are unsure as to the identity of these extra bands).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Immunogen | Produced in rabbits immunized with purified, recombinant Human GALNT2 (Accession#: NP_004472.1; Lys52-Gln571) |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GALNT2 |
| Purity | Antigen and protein A Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Antigen and protein A Affinity-purified |
![Immunohistochemistry Polypeptide GalNac Transferase 1/GALNT1 Antibody [Unconjugated]](https://images.novusbio.com/images2/GALNT1_AF7140_Immunohistochemistry_12224.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | GALNT2 |
![Immunocytochemistry/Immunofluorescence: GALNT2 Antibody [NBP3-00188] - Immunofluorescence staining of GALNT2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human GALNT2 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/GALNT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-00188-img0001.jpg "Immunocytochemistry/Immunofluorescence: GALNT2 Antibody [NBP3-00188] - Immunofluorescence staining of GALNT2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human GALNT2 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.")
![Immunohistochemistry-Paraffin: GALNT2 Antibody [NBP3-00188] - Immunochemical staining of human GALNT2 in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/GALNT2-Antibody-Immunohistochemistry-Paraffin-NBP3-00188-img0004.jpg "Immunohistochemistry-Paraffin: GALNT2 Antibody [NBP3-00188] - Immunochemical staining of human GALNT2 in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).")
![Immunoprecipitation: GALNT2 Antibody [NBP3-00188] - GALNT2 was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. Lane C: 0.5 mg U87MG Whole Cell Lysate. 4 ul anti-GALNT2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-GALNT2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 71 kDa](http://images.novusbio.com/fullsize/GALNT2-Antibody-Immunoprecipitation-NBP3-00188-img0005.jpg "Immunoprecipitation: GALNT2 Antibody [NBP3-00188] - GALNT2 was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. Lane C: 0.5 mg U87MG Whole Cell Lysate. 4 ul anti-GALNT2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-GALNT2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 71 kDa")
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![N/A Angiopoietin-like Protein 3/ANGPTL3 [HRP]](https://images.novusbio.com/images2/DATA_Angiopoietinlike_Protein_3_DANL30_ELISA_575.jpg)
![Immunohistochemistry Lipoprotein Lipase/LPL Antibody [Unconjugated]](https://images.novusbio.com/images2/Lipoprotein_Lipase_AF7197_Immunohistochemistry_21186.jpg)
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![Immunohistochemistry Sortilin Antibody [Unconjugated]](https://images.novusbio.com/images2/Sortilin_AF2934_Immunohistochemistry_7161.jpg)
![Western Blot Sortilin Antibody [Unconjugated]](https://images.novusbio.com/images2/Sortilin_AF2934_Western_Blot_19100.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")