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TATA Element Modulatory Factor 1 Antibody

Images

 
Genetic Strategies: Western Blot: TATA Element Modulatory Factor 1 Antibody [NBP1-90117] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TMF1 ...read more
Immunocytochemistry/ Immunofluorescence: TATA Element Modulatory Factor 1 Antibody [NBP1-90117] - Staining of human cell line U-2 OS shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TATA Element Modulatory Factor 1 Antibody [NBP1-90117] - Staining of human gallbladder shows strong cytoplasmic positivity in glandular cells.
Western Blot: TATA Element Modulatory Factor 1 Antibody [NBP1-90117] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

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TATA Element Modulatory Factor 1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TSTTSDIEVLDHESVISESSASSRQETTDSKSSLHLMQTSFQLLSASACPEYNRLDDFQKLTESCCSSDAFERIDSFSVQSLDSRSVSEINSDDELSGKGYALVPIIVNSSTPKSKTVESAEGKSEEVNETLVIPTEEAEMEESG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TMF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
TATA Element Modulatory Factor 1 Protein (NBP1-90117PEP)
Publications
Read Publications using
NBP1-90117 in the following applications:

Reactivity Notes

Reactivity reported in scientific literature (PMID: 22553199)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TATA Element Modulatory Factor 1 Antibody

  • TATA element modulatory factor 1

Background

Potential coactivator of the androgen receptor. Mediates STAT3 degradation. May play critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. This protein binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TATA Element Modulatory Factor 1 Antibody (NBP1-90117)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TATA Element Modulatory Factor 1 Antibody (NBP1-90117) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TMF1