GALK1 Antibody - Azide and BSA Free

Images

 
Genetic Strategies: Western Blot: GALK1 Antibody [NBP2-99783] - Anti-GALK1 rabbit polyclonal antibody at 1:500 dilution. Lane A: GALK1 knockout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate ...read more
Immunocytochemistry/ Immunofluorescence: GALK1 Antibody [NBP2-99783] - Immunofluorescence staining of HG3C-GALK1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% ...read more
Western Blot: GALK1 Antibody [NBP2-99783] - Anti-GALK1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) ...read more
Immunoprecipitation: GALK1 Antibody [NBP2-99783] - GALK1 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate Lane B: 0.5 mg HepG2 Whole Cell Lysate 4 ul anti-GALK1 rabbit polyclonal antibody and 15 ul of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Validated by:
     

Genetic Strategies

   

Order Details

GALK1 Antibody - Azide and BSA Free Summary

Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Immunogen
Produced in rabbits immunized with purified, recombinant Human GALK1 (Accession#: P51570; Met1-Leu392)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GALK1
Purity
Antigen and protein A Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:300-1:10000
  • Immunoprecipitation 1-4 uL/mg of lysate
  • Knockout Validated
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 um filtered solution in PBS
Preservative
No Preservative
Purity
Antigen and protein A Affinity-purified

Alternate Names for GALK1 Antibody - Azide and BSA Free

  • EC 2.7.1.6
  • galactokinase 1
  • galactokinase
  • Galactose kinase
  • GALK
  • GALK1
  • GK1
  • HEL-S-19

Background

The GALK1 gene encodes for a major enzyme involved in the metabolism of galactose. The protein coded is 392 amino acids long at a mass of around 42 kDA. A lack of the protein coded by the GALK1 gene results in infantile congenital cataracts and adult presenile cataracts. The GALK1 gene has also been linked to pneumonia, jaundice, hepatoblastoma, citrin deficiency, galactosemia, down syndrome, liver disease, ovarian cancer, and mevalonic aciduria. It interacts with DMWD, OTUD4, ATG2A, ATG10, AGO4 to function in galactose metabolism, the Leloir pathway, and colanic acid building blocks biosynthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GALK1 Antibody (NBP2-99783) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GALK1