I would like to know in which recombinant system you produce recombinant human GAD65?

Name: Recombinant Human GAD2/GAD65 GST (N-Term) Protein
Brand: Novus Biologicals
SKU: H00002572-P02
Rating: 5 (1 reviews)

Question

Accepted Answer

H00002572-P02 is produced using Abnova's cell-free wheat germ expression system and contains a 26 kDa N-terminal GST-tag.

Reactivity	HuSpecies Glossary
Applications	WB, ELISA, MA, AP, ELISA

Description	A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-585 of Human GAD2 Source: Wheat Germ (in vitro) Amino Acid Sequence: MASPGSGFWSFGSEDGSGDSENPGTARAWCQVAQKFTGGIGNKLCALLYGDAEKPAESGGSQPPRAAARKAACACDQKPCSCSKVDVNYAFLHATDLLPACDGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWELADQPQNLEEILMHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWLTSTANTNMFTYEIAPVFVLLEYVTLKKMREIIGWPGGSGDGIFSPGGAISNMYAMMIARFKMFPEVKEKGMAALPRLIAFTSEHSHFSLKKGAAALGIGTDSVILIKCDERGKMIPSDLERRILEAKQKGFVPFLVSATAGTTVYGAFDPLLAVADICKKYKIWMHVDAAWGGGLLMSRKHKWKLSGVERANSVTWNPHKMMGVPLQCSALLVREEGLMQNCNQMHASYLFQQDKHYDLSYDTGDKALQCGRHVDVFKLWLMWRAKGTTGFEAHVDKCLELAEYLYNIIKNREGYEMVFDGKPQHTNVCFWYIPPSLRTLEDNEERMSRLSKVAPVIKARMMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL
Preparation Method	in vitro wheat germ expression system
Details of Functionality	This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
Source	Wheat germ
Protein/Peptide Type	Recombinant Protein
Gene	GAD2
Purity	>80% by SDS-PAGE and Coomassie blue staining

Storage	Store at -80C. Avoid freeze-thaw cycles.
Buffer	50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative	No Preservative
Purity	>80% by SDS-PAGE and Coomassie blue staining

Publications using H00002572-P02	Applications	Species
Pang Z, Kushiyama A, Sun J et al. Glial fibrillary acidic protein (GFAP) is a novel biomarker for the prediction of autoimmune diabetes. FASEB J 2017-05-25 [PMID: 28546444]
Grupillo M, Gualtierotti G, He J et al. Essential roles of insulin expression in Aire+ tolerogenic dendritic cells in maintaining peripheral self-tolerance of islet beta-cells. Cell Immunol. 2012-01-12 [PMID: 22297234]
Fan Y, Rudert WA, Grupillo M et al. Thymus-specific deletion of insulin induces autoimmune diabetes. EMBO J. 2009-08-13 [PMID: 19680229]

Recombinant Human GAD2/GAD65 GST (N-Term) Protein