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Frizzled-3 Antibody

Images

 
Western Blot: Frizzled-3 Antibody [NBP1-30940] - Western blot analysis of Frizzled-3 Antibody on NIH-3T3, diluted at 1:1000 with 7.5% SDS PAGE gel.
Western Blot: Frizzled-3 Antibody [NBP1-30940] - Western blot analysis of Frizzled-3 Antibody on HeLa S3 (lane A), MOLT4 (lane B), and Raji (lane C), diluted at 1:1000 with 7.5% SDS PAGE gel.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, RM, XpSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Frizzled-3 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Frizzled-3. The exact sequence is proprietary.
Localization
Membrane, Multi-pass membrane protein
Specificity
Antibody reactive against peptide, detects immunogen. Further validation in progress.
Predicted Species
Rat (100%), Chicken (100%), Xenopus (100%), Rhesus Macaque (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FZD3
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Antibody reactive against peptide, detects immunogen. Further validation in progress.
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Frizzled-3 Antibody

  • frizzled (Drosophila) homolog 3
  • frizzled homolog 3 (Drosophila)
  • Frizzled3
  • Frizzled-3
  • Fz-3
  • FZD3
  • hFz3

Background

This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. The function of this protein is unknown, although it may play a role in mammalian hair follicle development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FZD3
Entrez
Uniprot