Novus Biologicals products are now on bio-techne.com

FOXO3 Antibody (648716) [Unconjugated]

Images

 
Image 1 of 2
(
Enlarge)
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clone
648716
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

  • Size / Catalog#QtyPrice
    MAB6165-SP
    25 ug
    $149.00
    5-7 Business Days
    MAB6165
    100 ug
    $459.00
    1-3 Business Days
    View Available Formulations
    Catalog# & Formulation Size Price
Shipping $45.00 per order
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Datasheet
Reviews & Publications
Protocols & FAQs
Support & Research

FOXO3 Antibody (648716) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human FoxO3
Ala373-Gly673
Accession # O43524
Specificity
Detects human and mouse FoxO3 in Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
FOXO3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using
MAB6165 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FOXO3 Antibody (648716) [Unconjugated]

  • AF6q21 protein
  • AF6q21
  • DKFZp781A0677
  • FKHRL1
  • FKHRL1MGC31925
  • FKHRL1P2
  • forkhead box O3
  • forkhead box protein O3
  • forkhead homolog (rhabdomyosarcoma) like 1
  • Forkhead in rhabdomyosarcoma-like 1
  • forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
  • FOXO2
  • FoxO3
  • FOXO3A
  • FOXO3Aforkhead box O3A
  • MGC12739

Background

Forkhead box O3 (FoxO3) is a ubiquitously expressed transcriptional regulator that is involved in cellular differentiation, angiogenesis, tumor progression, apoptosis, and the responses to oxidative stress and DNA damage. Phosphorylation of FoxO3 by Akt induces its association with 14-3-3 proteins and its retention in the cytoplasm. In response to the loss of survival factors, dephosphorylation of FoxO3 induces its translocation to the nucleus where it promotes apoptosis. Its level of acetylation is regulated in response to cellular metabolic requirements. Within amino acids 372‑673 (C-terminal to the DNA binding domain), human FoxO3 shares 95% aa sequence identity with mouse and rat FoxO3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NBP2-31376
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information


Product PDFs

  PDF SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our FOXO3 Antibody (648716) [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol FOXO3
Uniprot