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Recombinant Rat FGF18 Protein

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SDS-Page: Rat FGF18 Protein [NBP2-35296]
SDS-Page: Rat FGF18 Protein [NBP2-35296]

Product Details

Summary
Reactivity RtSpecies Glossary
Applications PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier-Free
NBP2-35296-1mg
Carrier-Free
NBP2-35296-100ug
Carrier-Free
NBP2-35296-500ug
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Recombinant Rat FGF18 Protein Summary

Description
A single non-glycosylated polypeptide chain containing 180 amino acids corresponding to FGF18 Source: E. coli

Uniprot ID: O88182

Amino Acid Sequence: EENVDFRIHV ENQTRARDDV SRKQLRLYQL YSRTSGKHIQ VLGRRISARG EDGDKYAQLL VETDTFGSQV RIKGKETEFY LCMNRKGKLV GKPDGTSKEC VFIEKVLENN YTALMSAKYS GWYVGFTKKG RPRKGPKTRE NQQDVHFMKR YPKGQTELQK PFKYTTVTKR SRRIRPTHPG

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Details of Functionality
FGF18 Protein is fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 10^6 IU/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
FGF18
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of FGF18 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-35296 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 500 mM NaCl.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Rat FGF18 Protein

  • FGF18
  • FGF-18
  • FGF-18ZFGF5
  • fibroblast growth factor 18
  • zFGF5

Background

Rat FGF-18 is encoded by the FGF18 gene in rats. By phylogenetic analysis and gene location analysis, FGF-18 is divided into FGF-8 subfamily which has three members FGF-8, FGF-17 and FGF-18. Using FGF knockout mice model, the numbers of this subfamily were testified that have crucial roles of in embryo development. FGF-18-/- mice have decreased expression of osteogenic markers and delayed long-bone ossification. FGF-18 also has significant roles in lung development and has an anabolic effect on cartilage formation. Additionally, it has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. Rat FGF-18 shares 98% amino acid sequence identity with human.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Recombinant Rat
FGF18 Protein
NBP2-35296
Recombinant Rat FGF18 Protein
Species: Rt
Applications: PAGE, Bioactivity

Publications for FGF18 Protein (NBP2-35296)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: Bioactivity.


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Bioinformatics

Gene Symbol FGF18