I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?

Name: FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-70718
Price: 569 USD

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Accepted Answer

Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have several antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our  NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, RtSpecies Glossary
Applications	WB, ICC/IF, IHC
Clone	OTI3D9
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Format	Azide and BSA Free
Concentration	LYOPH

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NBP2-70718AF350

Alexa Fluor 405 NBP2-70718AF405

Alexa Fluor 488 NBP2-70718AF488

Alexa Fluor 532 NBP2-70718AF532

Alexa Fluor 594 NBP2-70718AF594

Alexa Fluor 647 NBP2-70718AF647

Alexa Fluor 700 NBP2-70718AF700

Alexa Fluor 750 NBP2-70718AF750

Allophycocyanin NBP2-70718APC

Biotin NBP2-70718B

CoraFluor 1 NBP2-70718CL1

DyLight 350 NBP2-70718UV

DyLight 405 NBP2-70718V

DyLight 488 NBP2-70718G

DyLight 550 NBP2-70718R

DyLight 594 NBP2-70718DL594

DyLight 650 NBP2-70718C

DyLight 680 NBP2-70718FR

DyLight 755 NBP2-70718IR

FITC NBP2-70718F

HRP NBP2-70718H

Janelia Fluor 525 NBP2-70718JF525

Janelia Fluor 549 NBP2-70718JF549

Janelia Fluor 646 NBP2-70718JF646

Janelia Fluor 669 NBP2-70718JF669

PE NBP2-70718PE

PerCP NBP2-70718PCP

Unconjugated NBP1-47749

Catalog# & Formulation	Size	Price
Standard Formulation NBP1-47749

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-70718AF350
Alexa Fluor 405	NBP2-70718AF405
Alexa Fluor 488	NBP2-70718AF488
Alexa Fluor 532	NBP2-70718AF532
Alexa Fluor 594	NBP2-70718AF594
Alexa Fluor 647	NBP2-70718AF647
Alexa Fluor 700	NBP2-70718AF700
Alexa Fluor 750	NBP2-70718AF750
Allophycocyanin	NBP2-70718APC
Biotin	NBP2-70718B
CoraFluor 1	NBP2-70718CL1
DyLight 350	NBP2-70718UV
DyLight 405	NBP2-70718V
DyLight 488	NBP2-70718G
DyLight 550	NBP2-70718R
DyLight 594	NBP2-70718DL594
DyLight 650	NBP2-70718C
DyLight 680	NBP2-70718FR
DyLight 755	NBP2-70718IR
FITC	NBP2-70718F
HRP	NBP2-70718H
Janelia Fluor 525	NBP2-70718JF525
Janelia Fluor 549	NBP2-70718JF549
Janelia Fluor 646	NBP2-70718JF646
Janelia Fluor 669	NBP2-70718JF669
PE	NBP2-70718PE
PerCP	NBP2-70718PCP
Unconjugated	NBP1-47749

FGF basic/FGF2/bFGF Antibody (OTI3D9) - Azide and BSA Free

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Bioinformatics

Immunogen	Human recombinant protein fragment corresponding to amino acids 10-155 of human bFGF (NP_001997) produced in E.coli.
Isotype	IgG2b
Clonality	Monoclonal
Host	Mouse
Gene	FGF2
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Dilutions	Immunocytochemistry/ Immunofluorescence 1:100 Immunohistochemistry 1:50 Immunohistochemistry-Paraffin Western Blot 1:1000
Theoretical MW	30.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Storage	Store at -20C. Avoid freeze-thaw cycles.
Buffer	Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative	No Preservative
Concentration	LYOPH
Purity	Immunogen affinity purified
Reconstitution Instructions	we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.