Western Blot: FGF basic/FGF2 Antibody (3D9) [NBP1-47749] - Analysis of extracts (10ug) from 4 different cell lines by using anti-BFGF monoclonal antibody at 1:200 dilution
Immunocytochemistry/ Immunofluorescence: FGF basic/FGF2 Antibody (3D9) [NBP1-47749] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BFGF.
Immunohistochemistry-Paraffin: FGF basic/FGF2 Antibody (3D9) [NBP1-47749] - Staining of paraffin-embedded Human pancreas tissue using anti-BFGF mouse monoclonal antibody.
Western Blot: FGF basic/FGF2 Antibody (3D9) [NBP1-47749] - FGF-2 antibody ( 3D9 ) at 1:1000 + Recombinant human bFGF.
For IHC-P: Heat-induced epitope retrieval with 10mM Sodium Citrate Buffer (pH 6.0 at 100C for 10min is recommended.
Theoretical MW
30.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NBP1-47749 in the following applications:
Mouse reactivity reported in scientific literature (PMID: 27007053). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Packaging, Storage & Formulations
Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified
Alternate Names for FGF basic/FGF2/bFGF Antibody (OTI3D9)
basic fibroblast growth factor bFGF
Basic fibroblast growth factor
bFGF
FGF basic
FGF2
FGF-2
FGFBprostatropin
fibroblast growth factor 2 (basic)
HBGF-2
heparin-binding growth factor 2
Prostatropin
Background
BFGF is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for FGF basic/FGF2/bFGF Antibody (NBP1-47749). (Showing 1 - 1 of 1 FAQs).
I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have several antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we
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![Western Blot: FGF basic/FGF2 Antibody (3D9) [NBP1-47749] - Analysis of extracts (10ug) from 4 different cell lines by using anti-BFGF monoclonal antibody at 1:200 dilution](http://images.novusbio.com/fullsize/FGF-basic-FGF2-Antibody-3D9-Western-Blot-NBP1-47749-img0008.jpg "Western Blot: FGF basic/FGF2 Antibody (3D9) [NBP1-47749] - Analysis of extracts (10ug) from 4 different cell lines by using anti-BFGF monoclonal antibody at 1:200 dilution")
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![Immunocytochemistry/Immunofluorescence: FGF basic/FGF2 Antibody (3D9) [NBP1-47749] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BFGF.](http://images.novusbio.com/fullsize/FGF-basic-FGF2-Antibody-3D9-Immunocytochemistry-Immunofluorescence-NBP1-47749-img0005.jpg "Immunocytochemistry/Immunofluorescence: FGF basic/FGF2 Antibody (3D9) [NBP1-47749] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BFGF.")
![Immunohistochemistry-Paraffin: FGF basic/FGF2 Antibody (3D9) [NBP1-47749] - Staining of paraffin-embedded Human pancreas tissue using anti-BFGF mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FGF-basic-FGF2-Antibody-3D9-Immunohistochemistry-Paraffin-NBP1-47749-img0006.jpg "Immunohistochemistry-Paraffin: FGF basic/FGF2 Antibody (3D9) [NBP1-47749] - Staining of paraffin-embedded Human pancreas tissue using anti-BFGF mouse monoclonal antibody.")
![Western Blot: FGF basic/FGF2 Antibody (3D9) [NBP1-47749] - FGF-2 antibody ( 3D9 ) at 1:1000 + Recombinant human bFGF.](http://images.novusbio.com/fullsize/FGF-basic-FGF2-Antibody-3D9-Western-Blot-NBP1-47749-img0007.jpg "Western Blot: FGF basic/FGF2 Antibody (3D9) [NBP1-47749] - FGF-2 antibody ( 3D9 ) at 1:1000 + Recombinant human bFGF.")
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