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FGF-8 Antibody (2A10)

Images

 
Western Blot: FGF-8 Antibody (2A10) [H00002253-M01] - FGF8 monoclonal antibody (M01), clone 2A10. Analysis of FGF8 expression in NIH/3T3.
Western Blot: FGF-8 Antibody (2A10) [H00002253-M01] - FGF8 monoclonal antibody (M01), clone 2A10. Analysis of FGF8 expression in PC-12.
Western Blot: FGF-8 Antibody (2A10) [H00002253-M01] - FGF8 monoclonal antibody (M01), clone 2A10 Analysis of FGF8 expression in HepG2.
Western Blot: FGF-8 Antibody (2A10) [H00002253-M01] - FGF8 monoclonal antibody (M01), clone 2A10. Analysis of FGF8 expression in Raw 264.7.
Western Blot: FGF-8 Antibody (2A10) [H00002253-M01] - FGF8 monoclonal antibody (M01), clone 2A10. Analysis of FGF8 expression in Jurkat.
Sandwich ELISA: FGF-8 Antibody (2A10) [H00002253-M01] - Detection limit for recombinant GST tagged FGF8 is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA
Clone
2A10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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FGF-8 Antibody (2A10) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
FGF8 (NP_149354, 65 a.a. ~ 133 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SRRLIRTYQLYSRTSGKHVQVLANKRINAMAEDGDPFAKLIVETDTFGSRVRVRGAETGLYICMNKKGK
Specificity
FGF8 (2A10)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FGF8
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate for WB. It has been used for ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for FGF-8 Antibody (2A10)

  • AIGF
  • AIGFKAL6
  • Androgen-induced growth factor
  • FGF8
  • FGF-8
  • fibroblast growth factor 8 (androgen-induced)
  • fibroblast growth factor 8
  • HBGF-8
  • Heparin-binding growth factor 8
  • MGC149376

Background

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is known to be a factor that supports androgen and anchorage independent growth of mammary tumor cells. Overexpression of this gene has been shown to increase tumor growth and angiogensis. The adult expression of this gene is restricted to testes and ovaries. Temporal and spatial pattern of this gene expression suggests its function as an embryonic epithelial factor. Studies of the mouse and chick homologs revealed roles in midbrain and limb development, organogenesis, embryo gastrulation and left-right axis determination. The alternative splicing of this gene results in four transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for FGF-8 Antibody (H00002253-M01) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FGF8
Entrez
OMIM
Uniprot