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FGF-13 Antibody (S235-22) - Pan Specific

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Western Blot: FGF-13 Antibody (S235-22) - Pan Specific [NBP2-42178] - Western Blot analysis of Rat Brain Membrane showing detection of ~30 kDa FGF-13 (pan) protein using Mouse Anti-FGF-13 (pan) Monoclonal Antibody, Clone S235-22 (NBP2-42178). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-FGF-13 (pan) Monoclonal Antibody (NBP2-42178) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~30 kDa.
Western Blot: FGF-13 Antibody (S235-22) - Pan Specific [NBP2-42178] - Western Blot analysis of Rat Brain Membrane showing detection of ~30 kDa FGF-13 (pan) protein using Mouse Anti-FGF-13 (pan) Monoclonal Antibody, ...read more
Immunocytochemistry/ Immunofluorescence: FGF-13 Antibody (S235-22) - Pan Specific [NBP2-42178] - Immunocytochemistry/ Immunofluorescence analysis using Mouse Anti-FGFA/FHFA (pan) Monoclonal Antibody, Clone S235-22 ...read more
Immunocytochemistry/ Immunofluorescence: FGF-13 Antibody (S235-22) - Pan Specific [NBP2-42178] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
S235-22
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP2-42178AF405
Alexa Fluor 488
NBP2-42178AF488
Alexa Fluor 647
NBP2-42178AF647
Alexa Fluor 700
NBP2-42178AF700
Biotin
NBP2-42178B
DyLight 350
NBP2-42178UV
DyLight 405
NBP2-42178V
DyLight 488
NBP2-42178G
DyLight 550
NBP2-42178R
DyLight 650
NBP2-42178C
DyLight 680
NBP2-42178FR
DyLight 755
NBP2-42178IR
FITC
NBP2-42178F
HRP
NBP2-42178H
Janelia Fluor 549
NBP2-42178JF549
Janelia Fluor 646
NBP2-42178JF646
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FGF-13 Antibody (S235-22) - Pan Specific Summary

Immunogen
Synthetic peptide amino acids 2-18 (AAAIASSLIRQKRQARE) of human FHF2A. 100% identical to rat, 94% identical to mouse. >80% identity with FGF12A/FHF1A, FGF14A/FHF4A and FGF11A/FHF3A.
Localization
Cell Projection , Growth Cone , Dendrite , Nucleus , Cytoplasm
Specificity
Greater then 80% identity with FGF12A/FHF1A, FGF14A/FHF4A and FGF11A/FHF3A. Detects approx 30kDa. Does not cross-react with FGF13B/FHF2B. Cross reacts with FGF12A/FHF1A and FGF14A/FHF4A.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
FGF13
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot 1:1000
Application Notes
1 ug/ml of FGFA/FHFA (pan) Antibody was sufficient for detection of FGFA/FHFA (pan) in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Reactivity Notes

Immunogen displays sequence identity for non-tested species: Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 50% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for FGF-13 Antibody (S235-22) - Pan Specific

  • FGF13
  • FGF-13
  • FGF2Fibroblast growth factor homologous factor 2
  • FHF2FHF-2
  • fibroblast growth factor 13

Background

FGF13 (fibroblast growth factor 13), belongs to the FGF family which are involved in cell growth, tissue repair, embryonic development and a number of other biological processes. FGF13 may also play a role in nervous system development/function. The location of this gene on chromosome X has been studied for links to Borjeson-Forssman-Lehmann syndrome (BFLS). FGF13 is known to have interactions with MAPK8IP2, SCN8A, CLEC3B, PRNP and EGFR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FGF-13 Antibody (S235-22) - Pan Specific
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Bioinformatics

Gene Symbol FGF13
Uniprot