Human Fatty Acid Synthase/FASN ELISA Kit (Colorimetric) Summary
Specificity |
This kit recognizes Human Fatty Acid Synthase/FASN in samples. No Significant cross-reactivity or interference between Human Fatty Acid Synthase/FASN and analogues was observed. |
Standard Curve Range |
0.31 - 20 ng/mL |
Sensitivity |
0.19 ng/mL |
Assay Type |
Sandwich-ELISA |
Inter-Assay |
CV% < 4.8 |
Intra-Assay |
CV% < 4.66 |
Spike Recovery |
86-100% |
Sample Volume |
100 ul |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
FASN |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
|
Alternate Names for Human Fatty Acid Synthase/FASN ELISA Kit (Colorimetric)
Background
Fatty Acid Synthase (FAS) is a very large enzymatic system involved in de novo lipogenesis (triglyceride production). Specifically, FAS catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. It is also one of the accepted markers for insulin resistance and SREBP, LXR activation.
In some cancer cell lines, FAS has been found to be fused with ER-alpha. Therefore, FAS antibodies are useful tools for studies on fatty acid synthesis and research on certian cancers.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Publications for Fatty Acid Synthase/FASN ELISA Kit (NBP2-82512)(1)
Showing Publication 1 -
1 of 1.
Publication using NBP2-82512 |
Applications |
Species |
Michele Dei Cas, Chiara Maura Ciniselli, Elisabetta Vergani, Emilio Ciusani, Mariachiara Aloisi, Valeria Duroni, Paolo Verderio, Riccardo Ghidoni, Rita Paroni, Paola Perego, Giovanni Luca Beretta, Laura Gatti, Monica Rodolfo, Alexander N. Orekhov, Vasily Nikolaevich Sukhorukov Alterations in Plasma Lipid Profiles Associated with Melanoma and Therapy Resistance International Journal of Molecular Sciences 2024-01-26 [PMID: 38338838] |
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