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FARS2 Antibody

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Western Blot: FARS2 Antibody [NBP1-85187] - Analysis in control (vector only transfected HEK293T lysate) and FARS2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: FARS2 Antibody [NBP1-85187] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
Immunohistochemistry-Paraffin: FARS2 Antibody [NBP1-85187] - Staining of human kidney shows strong cytoplasmic and extra cellular positivity in cells in tubules.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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FARS2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: CYFPFTHPSFEMEINFHGEWLEVLGCGVMEQQLVNSAGAQDRIGWAFGLGLERLAMILYDIPDIRLFWCEDERFLKQFCVSNINQKVKFQPLSKYPAVINDISFWLPSENYAEND
Predicted Species
Mouse (92%), Rat (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FARS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
FARS2 Protein (NBP1-85187PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for FARS2 Antibody

  • dJ236A3.1
  • dJ520B18.2 (FARS1 (phenylalanine-tRNA synthetase))
  • dJ520B18.2
  • EC 6.1.1
  • EC 6.1.1.20
  • FARS1dJ236A3.1 (phenylalanine-tRNA synthetase)
  • HSPC320
  • phenylalanine translase
  • phenylalanine tRNA ligase 2, mitochondrial
  • Phenylalanine--tRNA ligase
  • phenylalanine-tRNA synthetase 1 (mitochondrial)
  • phenylalanine-tRNA synthetase 2 (mitochondrial)
  • phenylalanyl-tRNA synthetase 2, mitochondrial
  • phenylalanyl-tRNA synthetase, mitochondrial
  • PheRS

Background

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. This gene encodes a phenylalanine-tRNA synthetase (PheRS) localized to the mitochondrion which consists of a single polypeptide chain, unlike the (alpha-beta)2 structure of the prokaryotic and eukaryotic cytoplasmic forms of PheRS. Structure analysis and catalytic properties indicate mitochondrial PheRSs may constitute a class of PheRS distinct from the enzymes found in prokaryotes and in the eukaryotic cytoplasm. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol FARS2