Rat Factor VIII ELISA Kit (Colorimetric) Summary
Specificity |
This kit recognizes Rat Factor VIII in samples. No Significant cross-reactivity or interference between Rat Factor VIII and analogues was observed. |
Standard Curve Range |
7.81 - 500 pg/mL |
Sensitivity |
4.69 pg/mL |
Assay Type |
Sandwich-ELISA |
Inter-Assay |
CV% < 5.64 |
Intra-Assay |
CV% < 4.5 |
Spike Recovery |
84-104% |
Sample Volume |
100 ul |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
F8 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
|
Alternate Names for Rat Factor VIII ELISA Kit (Colorimetric)
Background
Von Willebrand Factor (VWF) was previously known as Factor VIII related antigen. VWF is synthesized exclusively by endothelial cells and megakaryocytes, and stored in the intracellular granules or constitutively secreted into plasma. This glycoprotein functions as both an antihemophilic factor carrier and a platelet vessel wall mediator in the blood coagulation system. Important in the maintenance of homeostasis, it participates in platelet vessel wall interactions by forming a noncovalent complex with coagulation factor VIII at the site of vascular injury. The Von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. Mutations in this gene or deficiencies in this protein result in Von Willebrand's disease. VWD is characterized by frequent bleeding (gingival, minor skin quantitative lacerations, menorrhagia, etc.).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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