| Reactivity | HuSpecies Glossary |
| Applications | WB, Simple Western, ICC/IF |
| Clonality | Polyclonal |
| Host | Sheep |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | E. coli-derived recombinant human Thymosin beta 4 Ser2-Ser44 Accession # P62328 |
| Specificity | Detects human Thymosin beta 4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Thymosin beta 10 and rhThymosin beta 16 is observed. |
| Source | N/A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Sheep |
| Gene | TMSB4X |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Sterile PBS to a final concentration of 0.2 mg/mL. |
Thymosin beta 4 (T beta 4; also TB4X and Fx) is a 5.0 kDa member of the beta -thymosin family of molecules. Members of this family range from 41-44 amino acids (aa) in length, and possess an isoelectric point that lies between pH 4.0-7.0 ( alpha -thymosins have values less than 4.0). Multiple cell types produce T beta 4, either constitutively, or after stimulation. They include platelets, endothelial cells, neutrophils, astrocytes and macrophages. T beta 4 is both a secreted and intracellular molecule. The secreted form contributes to wound healing and angiogenesis, and may act on ATPase. Intracellularly, it forms a 1:1 complex with G-actin and blocks F-actin polymerization. This regulates the availablility of actin monomers for filament formation and subsequent cell migration. Mature human T beta 4 is 43 aa in length (aa 2-44). It contains an actin-binding site (aa 17-23), one acetylated Ser and five acetylated lysines (4; 12; 26; 32; 39) and one phosphorylation site at Thr23. T beta 4 undergoes proteolytic processing to generate an N-terminal acetylated peptide (aa 2-5: SerAspLysPro). Mature human T beta 4 is identical to mouse T beta 14 in aa sequence, and it shares 74% aa identity with its human family member T beta 10.
Secondary Antibodies |
Isotype Controls |
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