![Western Blot: F-Spondin/SPON1 Antibody (PSH02-84) [NBP3-32352] - Western blot analysis of F-Spondin/SPON1 on different lysates with Rabbit anti-F-Spondin/SPON1 antibody (NBP3-32352) at 1/1,000 dilution. Lane 1: SW1990 cell lysate Lane 2: PANC-1 cell lysate Lane 3: U-2 OS cell lysate Lane 4: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 91 kDa Observed band size: 91120 kDa Exposure time: 1 minutes 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32352) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32352_rabbit-f-spondin-spon1-mab-psh02-84-278202415242685.jpg "Western Blot: F-Spondin/SPON1 Antibody (PSH02-84) [NBP3-32352] - Western blot analysis of F-Spondin/SPON1 on different lysates with Rabbit anti-F-Spondin/SPON1 antibody (NBP3-32352) at 1/1,000 dilution. Lane 1: SW1990 cell lysate Lane 2: PANC-1 cell lysate Lane 3: U-2 OS cell lysate Lane 4: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 91 kDa Observed band size: 91120 kDa Exposure time: 1 minutes 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32352) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF |
| Clone | PSH02-84 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Synthetic peptide within human Spondin-1 201-250 / 807. (Uniprot: Q9HCB6) |
| Localization | Secreted protein; extracellular space; extracellular matrix. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | SPON1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 91 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.05% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for F-Spondin/SPON1 Antibody (NBP3-32352)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SPON1 |
![Immunocytochemistry/ Immunofluorescence: F-Spondin/SPON1 Antibody (PSH02-84) [NBP3-32352] - Immunocytochemistry analysis of PANC-1 cells labeling F-Spondin/SPON1 with Rabbit anti-F-Spondin/SPON1 antibody (NBP3-32352) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-F-Spondin/SPON1 antibody (NBP3-32352) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32352_rabbit-f-spondin-spon1-mab-psh02-84-29202410234238.jpg "Immunocytochemistry/ Immunofluorescence: F-Spondin/SPON1 Antibody (PSH02-84) [NBP3-32352] - Immunocytochemistry analysis of PANC-1 cells labeling F-Spondin/SPON1 with Rabbit anti-F-Spondin/SPON1 antibody (NBP3-32352) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-F-Spondin/SPON1 antibody (NBP3-32352) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.")
![Flow Cytometry: F-Spondin/SPON1 Antibody (PSH02-84) [NBP3-32352] - Flow cytometric analysis of PANC-1 cells labeling F-Spondin/SPON1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32352, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32352_rabbit-f-spondin-spon1-mab-psh02-84-392024884651.jpg "Flow Cytometry: F-Spondin/SPON1 Antibody (PSH02-84) [NBP3-32352] - Flow cytometric analysis of PANC-1 cells labeling F-Spondin/SPON1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32352, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")